Osteosarcoma, one of the most common malignant bone tumours, is generally considered a differentiation disease caused by genetic and epigenetic disruptions in terminal osteoblasts. Novel therapies based on non-cytotoxic induction cell differentiation-responsive pathways could represent significant advance treating osteosarcoma; however, effective pharmaceuticals to induce are lacking. In present study, we investigated effect hyperoside, flavonoid compound, osteoblastic U2OS MG63 osteosarcoma...

Random-pattern skin flaps are widely used to close defects in reconstructive and plastic surgeries; however, they vulnerable necrosis, particularly the distal portion of flap.Here, we examined effects metformin on flap survival mechanisms underlying these effects.Following treatment, area, blood flow, number microvessels present were increased postoperative day 7, whereas tissue edema was reduced.In addition, promoted angiogenesis, inhibited apoptosis, relieved oxidative stress, autophagy...

Random skin flaps are often used in plastic surgery, but the complications of marginal flap ischemia and necrosis limit their wider clinical application. Apigenin (Api) is a flavonoid found various fruits vegetables. Api has been shown to promote angiogenesis, as well reduce oxidative stress, membrane damage, inflammation. In this study, we assessed effects treatment on random survival. Dorsal McFarlane were transplanted into rats, which randomly divided three groups: control (normal...

Skin flap transplantation is a common wound repair method in orthopedic surgery, but skin necrosis remains problematic. Memantine, an excitatory amino acid receptor antagonist, currently used the treatment of moderate to severe Alzheimer's disease, due its ability promote angiogenesis and reduce oxidative stress. This study investigated effect memantine on survival random flaps Sprague-Dawley (SD) rats. Thirty six male SD rats were divided into control, high-dose (20 mg/kg per day), low-dose...

Random skin flaps are routinely placed during plastic and reconstructive surgery, but the distal areas often develop ischemia necrosis. Baicalein, a major flavonoid extracted from traditional Chinese herbal medicine huangqin, Scutellaria baicalensis Georgi, may improve flap viability.Rats were randomly divided into baicalein control groups they underwent placement of modified McFarlane after intraperitoneal administration or vehicle. Flap survival water content measured 7 days later, as...

Hypoxia-inducible factor-1α (HIF-1α) plays a key role in angiogenesis, improves flap survival, and activates autophagy. The effect of HIF-1α-induced autophagy is still debatable. Thus, we investigated the on multiterritory perforator survival. In this study, 99 male Sprague-Dawley rats received procedure were divided into three groups with 33 each. dimethyloxalylglycine (DMOG) plus 3-methyladenine (3-MA) group intraperitoneal injection DMOG (40 mg/kg) 3-MA (10 mg/kg). control comparative...

<b>Background</b> Huangqi (<i>Radix astragali</i>) is a traditional Chinese drug, designed to "buqi," which means invigorating vital energy, widely used in clinical settings. We investigated the effect of injection on survival random skin flaps. <b>Methods</b> McFarlane flaps were established 60 rats divided into two groups. Postoperative celiac injections given both groups for 7 days. was injected test group, and saline controls. On day 7, tissues stained with hematoxylin eosin,...

The effect of vinpocetine on flap survival, vascular endothelial growth factor (VEGF) expression, and superoxide dismutase (SOD) malondialdehyde (MDA) contents were evaluated in this study. McFarlane model was established 20 rats within two groups. Postoperative celiac injection given for 7 days the groups: applied Group 1, same volume saline 2. Flap necrosis measured day by cellophane all VEGF expression determined using immunohistochemical methods tissue samples taken after injections. SOD...

BackgroundCyclooxygenase-2(COX-2) inhibitors provide desired analgesic effects after injury or surgery, but evidences suggested they also attenuate wound healing. The study is to investigate the effect of COX-2 inhibitor on random skin flap survival. MethodsThe McFarlane model was established in 40 rats and evaluated within two groups, each group gave same volume Parecoxib saline injection for 7 days. necrotic area measured, specimens were stained with haematoxylin-eosin(HE) histologic...

Random skin flaps are commonly used for wound repair and reconstruction. Electroacupuncture at The Zusanli point could enhance microcirculation blood perfusion in random flaps.To determine whether electroacupuncture can improve the survival of a rat model.Thirty-six male Sprague Dawley rats were randomly divided into 3 groups: control group (no electroacupuncture), Group A (electroacupuncture nonacupoint near point), B point). McFarlane established. On postoperative Day 2, malondialdehyde...

Abstract Detachment of adherent cells from extracellular matrix results in apoptosis, a process termed “anoikis”. Resistance to anoikis is implicated the progression many malignancies by facilitating migration and eventual colonization distant sites. Human kidney epithelial 293T, human osteoblast hFOB 1.19 osteosarcoma Saos‐2 significantly underwent when adherence was prevented. But MG‐63 were distinctly resistant detached. They formed large aggregates showed little apoptosis compared other...

Partial necrosis of skin flaps continues to restrict the survival local following plastic and reconstructive surgeries. The aim present study was investigate effects diammonium glycyrrhizinate (DG), a salt glycyrrhetinic acid that has been widely used in therapy chronic hepatitis human immunodeficiency virus infection, on random flap rats. McFarlane were established 60 male Sprague-Dawley rats randomly divided into three groups. Group I served as control group injected with saline (10 mg/kg)...

Objective To investigate the effect of Hirudin on random skin flap survival in rats.Methods 24 SD rats were randomly divided into control group and experimental group. The McFarlane flap(3 cm ×9 cm) rat models established dorsum. 3 ml (30 ATU) was injected group, while saline injection performed for 7 days. area two groups measured. tissue samples taken from proximal( Ⅰ ), middle( Ⅱ ) distal( Ⅲ portions flaps histologic study. VEGF bFGF expression also detected with immunohistochemistry...

Background and Purpose Skin flaps are among the most important means of wound repair in clinical settings. However, partial or even total distal necrosis may occur after a flap operation, with severe consequences for both patients doctors. This study investigated whether tert ‐butylhydroquinone (TBHQ), known agonist transcription factor nuclear erythroid 2‐related 2 (Nrf2), an antioxidant, could promote skin survival. Experimental Approach McFarlane models were established male...

The aim of this study was to investigate the relationship between cell adhesion and anoikis evasion among human osteosarcoma cells (MG-63), further molecular mechanisms.Human (MG-63) were assessed for apoptosis, caspase-3, E-cadherin β-catenin expression in EDTA control non-EDTA groups.MG-63 predominantly aggregated when suspension, suspended more dispersed group. Following culture suspension 24 h, 48 or 72 rates apoptosis 34.88%±3.64%, 59.3%±7.22% 78.5%±5.21% experimental group 7.34%±2.13%,...

Random-pattern skin flaps are widely used to close defects in reconstructive and plastic surgeries; however, they vulnerable necrosis, particularly the distal portion of flap. Here, we examined effects metformin on flap survival mechanisms underlying these effects. Following treatment, area, blood flow, number microvessels present were increased postoperative day 7, whereas tissue edema was reduced. In addition, promoted angiogenesis, inhibited apoptosis, relieved oxidative stress, autophagy...

Objective/Hypothesis: Random skin flap is commonly used for wound repairing and reconstruction. Electroacupuncture at Zuansanli (ST36) could effetely activate cholinergic system enhance microcirculation blood perfusion. The article aims to study the effects of electroacupuncture on random-pattern survival rate in a rat model. Materials Methods: Eighteen male Sprague-Dawley rats were randomly divided into 3 groups: control group (no postoperative electroacupuncture), A (postoperative...