Long non-coding RNA H19 (lncRNA H19) was found to be upregulated by hypoxia, its expression and function have never been tested in cerebral ischemia reperfusion (I/R) injury. This study intended investigate the role of lncRNA gene variation I/R injury with focusing on relationship autophagy activation. Cerebral induced rats middle artery occlusion followed reperfusion. SH-SY5Y cells were subjected oxygen glucose deprivation (OGD/R) simulate Real-time PCR, flow cytometry, immunofluorescence...

Abstract A 12 year old boy presented with 11 episodes of weakness, ataxia, nausea, slurred speech, dehydration, and sometimes severe lethargy bordering on coma. previously the had small intestinal resections leaving 20 cm bowel remaining. D-lactic acidosis was diagnosed basis a D-lactate level 5.23 mmol/l. The clinical presentation disease is recurrent unusual neurological manifestations metabolic acidosis. diagnosis dependent presentations plasma level. Development syndrome seems to be...

MicroRNAs (miRNAs) are under investigation as novel diagnostic and prognostic biomarkers in several malignancies, including gastric cancer (GC). miR-25 was overexpressed GC tissues, higher expression statistically correlated with aggressive clinicopathological characteristics. In our study, we investigate the associations of serum level characteristics, diagnosis prognosis patients.Serum samples from 184 patients, 56 gastritis patients 78 healthy controls were subjected to real-time...

Abstract Background Syndecan-1 (SDC-1) is a crucial membrane proteoglycan, which confirmed to participate in several tumor cell biological processes. However, the significance of SDC-1 colorectal carcinoma not yet clear. An objective this study was investigate role cells. Methods Expression tissues evaluated by Reverse transcription-quantitative real-time PCR (RT-qPCR) and western blot. After transfection with pcDNA3.1 or pc-SDC-1, efficiency measured. Next, SW480, SW620 LOVO viability,...

Colorectal cancer (CRC) is the fourth leading cause of cancer-induced mortality. Histone deacetylase 2 (HDAC2) involved in prognosis and therapy CRC. This study aimed to explore novel therapeutic targets for The alteration HDAC2 expression CRC tissues was estimated by qRT-PCR. After lentivirus transfection, knockdown confirmed western blot analysis. effect on cell proliferation then assessed 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Screened TargetScan,...

Here, we report an ultracompact angular displacement sensor based on the Talbot effect of optical microgratings. Periodic interference patterns were obtained behind upper grating. By putting another grating within region, total transmission two-grating structure was found to be approximatively in a linear relationship with relative pitch angle between two gratings, which explained by transversal shift patterns. The influence parameters (e.g., period, number lines and gap gratings) also...

Hiwi is well known for its role in stem cell renewal, maintaining the resting stage, and downregulating cycle of cells via RNA silencing. And overexpression has been recognized several types cancers. In present study, we examined expression colorectal cancer (CRC) specimens both mRNA protein levels real-time quantitative PCR, western blot assay, immunohistochemical staining. Then explored proliferation DNA methylation human CRC Caro-2 HT-29 lines. Results demonstrated that were significantly...

The aim of the present study was to investigate potential roles androgen receptor (AR) and matrix metalloproteinase (MMP)-2 MMP-9 in hepatocellular carcinoma (HCC) tissues whether their expression could be used as a predictor invasion stage cancer. levels AR, MMP-2 HCC adjacent tumor were measured by immunohistochemical staining assay. rates tissue 76.67, 73.33 76.67%, respectively, all which significantly higher than those tumor. these proteins represents local stage. have employed...

LncRNA (long non-coding RNA) H19 is a transcript of the gene that expressed during embryogenesis. We previously discovered role for circular lncRNA in onset and prognosis cerebral ischemic stroke. In this study, we used serum from patients with stroke, mouse cell culture models to elucidate roles plasma neuronal exosomes regulatory effect on insulin-like growth factor-1 its mechanism using western blotting, quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assays....

Inflammation is an essential driver of colorectal cancer (CRC). Identifying phenotypes and targets associated with inflammation may be effective way to treat CRC. R was used analyze interleukin 6 cytokine family signal transducer (IL6ST) expression in The Cancer Genome Atlas Colon Adenocarcinoma database. Immunohistochemistry, western blotting, quantitative PCR were detect IL6ST ferroptosis-related genes our cohort. Receiver operating characteristic curves evaluated the specificity...

Abstract Background Increasing the radiotherapy dose can result in improved local control for non-small-cell lung cancer (NSCLC) and thereby improve survival. Accelerated hypofractionated expose tumors to a high of radiation short period time, but optimal treatment regimen remains unclear. The purpose this study was evaluate feasibility utilizing high-dose accelerated three-dimensional conformal (at 3 Gy/fraction) with concurrent vinorelbine (NVB) carboplatin (CBP) chemotherapy advanced...

An increasing number of microRNA (miRNA) have been demonstrated to serve as molecular biomarkers for tumor cell progression. miR‑512‑5p was revealed oncogenic regulator in several types cancer. However, whether and how regulates non‑small lung cancer (NSCLC) remains unclear. In the present study, expression detected NSCLC tissues lines. Then, proliferation, migration, invasion apoptosis A549 H1299 lines were when overexpressed. Furthermore, underlying mechanism identified. The level...

<b>Background</b> Random skin flaps can be used throughout the hands and fingers. Thymosin β4 increase blood flow reduce ischemia–reperfusion injury; study was undertaken to investigate effect of thymosin on survival random flaps. <b>Methods</b> A total 45 male Sprague–Dawley rats were subjected a random-pattern operation. Rats randomly divided into three groups: control group (group A: intraperitoneal injection saline, 5 mg/kg/d) two treatment groups B: β4, single mg/kg dose per day) C:...

Abstract Background The competing endogenous RNA (ceRNA) networks of long non-coding RNAs (lncRNAs) and microRNAs (miRs) have aroused wide concerns. study aims to investigate the roles lncRNA DANCR-associated ceRNA network in growth behaviors colon cancer (CC) cells. Methods Differentially expressed lncRNAs between CC paracancerous tissues were analyzed using microarrays RT-qPCR. Follow-up studies conducted evaluate correlation DANCR expression prognosis patients. Loss-of-functions performed...

Patients with brain metastases from lung cancer have poor prognoses and short survival time, they are often excluded clinical trials. Whole-cranial irradiation is considered to be the standard treatment, but its efficacy not satisfactory. The purpose of this phase II trial was evaluate preliminary safety treatment whole-brain plus three-dimensional conformal boost combined concurrent topotecan for patients cancer.Patients metastasis received chemotherapy radiotherapy: conventional...

<b>Background</b> Huangqi (<i>Radix astragali</i>) is a traditional Chinese drug, designed to "buqi," which means invigorating vital energy, widely used in clinical settings. We investigated the effect of injection on survival random skin flaps. <b>Methods</b> McFarlane flaps were established 60 rats divided into two groups. Postoperative celiac injections given both groups for 7 days. was injected test group, and saline controls. On day 7, tissues stained with hematoxylin eosin,...

Ulcerative colitis is a chronic inflammatory disease of the colon where intestinal motility disturbed. Interstitial cells Cajal (ICC) are required to maintain normal motility. In present study, we assessed effect tumor necrosis factor-alpha (TNF-α) on viability and apoptosis ICC, as well expression stem cell factor (SCF), ghrelin, substance P. ICC were derived from small intestines Swiss albino mice. Cell measured using CCK-8 assay flow cytometry, respectively. ELISA was used measure...

Colorectal cancer (CRC) is identified as a primary cause of death around the world. The current chemotherapies are not cost-effective. Therefore, finding novel potential therapeutic target urgent. Titin (TTN) muscle protein that critical in hypertrophic cardiomyopathy. However, its role CRC well understood. study focused on exploring possible TTN carcinogenesis. mRNA and expression levels presented an obvious downregulation tissue samples, relative to normal control (p < 0.05). significantly...

Partial necrosis of skin flaps continues to restrict the survival local following plastic and reconstructive surgeries. The aim present study was investigate effects diammonium glycyrrhizinate (DG), a salt glycyrrhetinic acid that has been widely used in therapy chronic hepatitis human immunodeficiency virus infection, on random flap rats. McFarlane were established 60 male Sprague-Dawley rats randomly divided into three groups. Group I served as control group injected with saline (10 mg/kg)...

Aspirin is a novel anti-platelet drug that intensively recommended for the prevention and treatment of cerebral ischemic stroke. However, existence aspirin resistance weakens effects usually induces recurrence While mechanism underlying still unclear. Long non-coding RNA H19 (H19) closely associated with onset prognosis Since relationship between have never been reported, herein, we aimed to evaluate expression in aspirin-resistant stroke patients subsequently, ascertain ability diagnose...