A5100684726- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- Glioma Diagnosis and Treatment
- Cancer Mechanisms and Therapy
- RNA Research and Splicing
- Natural product bioactivities and synthesis
- FOXO transcription factor regulation
- Drug-Induced Adverse Reactions
- Ginseng Biological Effects and Applications
- Ovarian cancer diagnosis and treatment
- Venomous Animal Envenomation and Studies
- Autophagy in Disease and Therapy
- Epilepsy research and treatment
- Connective Tissue Growth Factor Research
- Cancer, Stress, Anesthesia, and Immune Response
- Monoclonal and Polyclonal Antibodies Research
- Natural Compounds in Disease Treatment
- Genomics and Chromatin Dynamics
- Vitamin D Research Studies
- Lymphoma Diagnosis and Treatment
- Ferroptosis and cancer prognosis
- Pregnancy and Medication Impact
- Infectious Diseases and Tuberculosis
Second Affiliated Hospital of Xi'an Jiaotong University
2017-2025
Obstetrics and Gynecology Hospital of Fudan University
2025
Huashan Hospital
2025
Children's Hospital of Fudan University
2025
Chang Gung Memorial Hospital
2020-2024
Second Affiliated Hospital of Jilin University
2011-2024
Henan Agricultural University
2024
Army Medical University
2019-2023
Xinqiao Hospital
2019-2023
Zhejiang Academy of Medical Sciences
2020
High-grade glioma (HGG) is a fatal human cancer. Bortezomib, proteasome inhibitor, has been approved for the treatment of multiple myeloma but its use in awaits further investigation. This study aimed to explore chemotherapeutic effect and underlying mechanism bortezomib on gliomas. U251 U87 cell viability proliferation were detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, tumor spheroid growth, colony formation assay. Cell apoptosis cycle flow...
Objectives Studies have reported the significance of circulating microRNAs (miRNAs) as biochemical markers cancer. Few reports demonstrate miRNAs in serum patients with ovarian epithelial carcinoma (EOC). The present study prospectively investigated status miRNA-92 (miR-92) EOC and healthy controls, examined potential usefulness this molecule a biomarker for diagnosis. Methods Levels miR-92 samples from controls were measured by quantitative real time reverse transcription–polymerase chain...
Abstract In the early stage of ovarian cancer (OC), molecular biomarkers are critical for its diagnosis and treatment. Nevertheless, there is little research on mechanism underlying tumorigenesis in OC. Herein, we aimed to explore whether long noncoding RNA (lncRNA) HAND2‐AS1 participated regulation cell proliferation, migration, apoptosis OC by regulating B‐cell lymphoma 2 like 11 (BCL2L11) microRNA‐340‐5p (miR‐340‐5p). Differentially expressed lncRNAs were screened microarray‐based...
Celastrus orbiculatus is used as a folk medicine in China for the treatment of numerous diseases. The ethyl acetate extract (COE) also displays wide range anti-cancer activities laboratory. However, effectiveness COE-induced autophagy and its mechanism action colorectal cancer cells have not been investigated thus far. present study analyzed effect COE on HT-29 cell viability, apoptosis using MTT assay, flow cytometry, transmission electron microscopy western blotting. Additionally,...
The aim of this study was to evaluate the long-term follow-up data Chinese children with self-limited focal epilepsy neonatal/infantile onset (SeLFE) and investigate clinical features, genetic background treatment outcomes type epileptic syndrome. We conducted a retrospective cohort twenty-six SeLFE admitted or followed by Department Pediatrics, Second Affiliated Hospital Xi'an Jiaotong University from October 2011 2021. Treatment decisions were based on children's seizure semiology,...
Recently, many studies have shown that microRNA (miR)-612 is involved in cancer progression. However, the role of miR-612 cervical remains unclear. The present study aims to investigate biological effects on cancer. expression tissues and cell lines was analysed by quantitative reverse transcription-polymerase chain reaction. effect proliferation, migration, invasion apoptosis studied appropriate methods. Protein determined Western blot analyses. Bioinformatics analysis luciferase reporter...
// Yang Lin 1 , Tianmin Xu Shunqing Zhou and Manhua Cui Department of Obstetrics Gynecology, The Second Hospital, Jilin University, Nanguan District, Changchun 130041, China Correspondence to: Cui, email: cuimanhua1326@163.com Keywords: microRNAs; miR-363; ovarian cancer; NOB1 Received: May 11, 2017 Accepted: September 04, Published: 30, 2017 ABSTRACT In this study, we investigated the role microRNA-363(miR-363) in cancer (OC) progression. MiR-363expression was...
Hiwi is well known for its role in stem cell renewal, maintaining the resting stage, and downregulating cycle of cells via RNA silencing. And overexpression has been recognized several types cancers. In present study, we examined expression colorectal cancer (CRC) specimens both mRNA protein levels real-time quantitative PCR, western blot assay, immunohistochemical staining. Then explored proliferation DNA methylation human CRC Caro-2 HT-29 lines. Results demonstrated that were significantly...
Context Shenmai Injection (SMI) is usually used to treat atherosclerotic coronary heart disease and viral myocarditis in China. However, the effect of SMI on multidrug resistance has not been reported.Objective To investigate reversal adriamycin (ADR) resistant breast cancer cell line (MCF-7/ADR) explore related molecular mechanisms.Materials methods The (0.25, 0.5, 1 mg/mL) reverse chemoresistance MCF-7/ADR cells was elucidated by MTT, HPLC-FLD, DAPI staining, flow cytometric analysis,...
Epithelial ovarian cancer (EOC) is a fatal disease for women due to lack of effective diagnostic biomarkers and therapeutic targets. Thus, it important identify develop specific markers formulate novel methods advanced recurrent cancer. We found that the receptor activated C-kinase 1 (RACK1) was elevated in most EOCs compared normal tissue, its expression levels correlated with key pathological characteristics including clinical stage metastasis by quantitative PCR immunohistochemistry. In...
The present study aimed to investigate the effect of downregulating cyclooxygenase‑2 (COX‑2) expression on growth human ovarian cancer cells. COX‑2‑specific small interfering RNA (siRNA) plasmid vector was constructed and then transfected into COX‑2 mRNA protein detected by quantitative polymerase chain reaction western blot analysis, respectively. Cell proliferation, apoptosis, cell cycle distribution migration were assessed following knockdown interference (RNAi). Western analysis used...
The present study is focused on expression of a target fusion protein which can be used in ovarian cancer therapy. It aimed to construct human urokinase-type plasminogen activator (uPA)1‑43-melittin eukaryotic vector express recombinant uPA1‑43-melittin (rhuPA1‑43-melittin) P. pastoris and detect its anticancer effects cells. DNA sequences that encode uPA1‑43 amino acids melittin were synthesized according native acid consequently inserted into pPICZαC vector. Then -pPICZαC was transformed...
To understand multidrug resistance gene expression in reactive astrocytes, the present study involved stimulated astrocytes with tumor necrosis factor (TNF)‑α and determined by reverse transcription‑quantitative polymerase chain reaction western blot analysis. Stimulation induced P‑glycoprotein (gp) resistance‑associated protein (MRP0) 1 expression, which peaked 24 h. Interestingly, of P‑gp Mrp1 correlated cell proliferation. Therefore, reactivation may increase through TNF‑α nuclear (NF)‑κB...
The aim of this study was to investigate the clinical characteristics and management malignant ovarian tumors during pregnancy, as well feto-maternal outcomes analyze influential factors on pregnancy outcomes.Eighty-five patients with malignancies treated at 12 tertiary hospitals between 2009 2019 were analyzed in study. features, histopathological characteristics, management, maternal perinatal retrospectively analyzed. features managements compared abortion group live birth group.The...
Vitamin D deficiency, a common occurrence among pregnant women, is an emerging public health concern worldwide. According to research, prenatal vitamin deficiency associated with various complications. This study assessed the status of women in Yanbian, Jilin Province, as well correlation and predictive value their levels relation gestational length (weeks) fetal weight, aiming provide basis for clinical diagnosis treatment. We conducted population-based retrospective involving 510 from...
Rosemary (Salvia rosmarinus) is a world-famous plant frequently subjected to various environmental stresses. Heat Shock Transcription Factor (HSF) has been shown be essential for growth and resistance This study utilized bioinformatics techniques identify the SrHSF gene family in rosemary genome. A total of 49 SrHSFs were detected, unevenly distributed across 12 chromosomes. The genes classifiable into 3 subfamilies contained 14 subgroups. They relatively conserved during evolutionary...
Rationale: Around 10%-20% patients with glioblastoma (GBM) are diagnosed more than one tumor lesions or multifocal GBM (mGBM). However, the understanding on genetic, DNA methylomic, and transcriptomic characteristics of mGBM is still limited. Methods: In this study, we collected nine foci from three followed by whole genome sequencing, bisulfite RNA immunohistochemistry. The data were further examined using public databases cell line. Results: Analysis genetic confirmed common features GBM,...
Abstract Background Multifocal glioblastoma is a rare type of with worse prognosis. In this article, we aimed to report two cases classical multifocal glioblastoma. Case presentation case 1, 47-year-old male presented dizziness, and once had sudden loss consciousness accompanied by convulsion limbs. Contrast-enhanced MRI showed multiple lesions heterogeneously ring-enhanced characters in the left hemisphere, diagnosed as He underwent craniotomy all lesions, concurrent radiotherapy...