A5045585462- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Polymer Surface Interaction Studies
- MicroRNA in disease regulation
- Marine Sponges and Natural Products
- Blood properties and coagulation
- Marine Biology and Environmental Chemistry
- Graphene and Nanomaterials Applications
- Berberine and alkaloids research
- RNA modifications and cancer
- Iron Metabolism and Disorders
- Plant-based Medicinal Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Lung Cancer Treatments and Mutations
- Nanoparticles: synthesis and applications
- Cell Adhesion Molecules Research
- Protein Interaction Studies and Fluorescence Analysis
- Nanofabrication and Lithography Techniques
- biodegradable polymer synthesis and properties
- Electrophoretic Deposition in Materials Science
- Ginseng Biological Effects and Applications
- Dendrimers and Hyperbranched Polymers
- Marine Ecology and Invasive Species
Huazhong University of Science and Technology
2024
Union Hospital
2024
Xiamen University
2016-2021
Massachusetts Institute of Technology
2013-2017
The University of Queensland
2009-2016
Nanyang Technological University
2014
Koch Institute for Integrative Cancer Research At MIT
2013-2014
Institute for Soldier Nanotechnologies
2013-2014
Allen Institute
2013
Jinan University
2012-2013
A single nanoparticle platform has been developed through the modular and controlled layer-by-layer process to codeliver siRNA that knocks down a drug-resistance pathway in tumor cells chemotherapy drug challenge highly aggressive form of triple-negative breast cancer. Layer-by-layer films were formed on nanoparticles by alternately depositing poly-l-arginine; bilayer surface could effectively load up 3500 molecules, resulting LbL exhibit an extended serum half-life 28 h. In animal models,...
Nanoparticles rapidly interact with the proteins present in biological fluids, such as blood. The that are adsorbed onto surface potentially dictate biokinetics of nanomaterials and their fate vivo. Using nanoparticles different sizes characteristics, studies have reported effects physicochemical properties on composition plasma proteins. However, to date, few been conducted focusing commonly exposed general public, metal oxides. previously established ultracentrifugation approaches,...
The binding of fibrinogen to various nanoparticles can result in protein unfolding and exposure cryptic epitopes that subsequently interact with cell surface receptors. This response is dependent on the size, charge, concentration nanoparticle. Here we examine kinetics human negatively charged poly(acrylic acid)-coated gold ranging size from 7 22 nm. These particles have previously been shown elicit an inflammatory cells. larger bound increasing affinity a slower dissociation rate. Each...
Nanoparticles containing two drugs released at different times effectively kill tumor cells.
Active targeting of nanoscale drug carriers can improve tumor-specific delivery; however, cellular heterogeneity both within and among tumor sites is a fundamental barrier to their success. Here, we describe microenvironment-responsive layer-by-layer (LbL) polymer carrier that actively targets tumors based on two independent mechanisms: pH-dependent uptake at hypoxic pH hyaluronan-directed cell-surface CD44 receptor, well-characterized biomarker for breast ovarian cancer stem cells. Hypoxic...
The binding of proteins to nanoparticles is an important event that can determine the biological effect in body. We examined plasma protein gold (5–20 nm) with different surface charge. Positively and negatively charged bound a range whereas neutral nanoparticle very little. As little as 25% polymer on inhibited binding, only slight change Fibrinogen high affinity both nanoparticles. However, kinetics protease digestion suggested orientation for each was different. Only induced cytokine...
While plasma proteins can influence the physicochemical properties of nanoparticles, adsorption protein to surface nanomaterials also alter structure and function protein. Here, we show that form a hard corona around synthetic layered silicate nanoparticles (LSN) one principle is serum albumin. The was required for recognition by scavenger receptors, major receptor family associated with mononuclear phagocyte system (MPS). Albumin alone could direct nanoparticle uptake human macrophages,...
Inorganic nanostructures have been used extensively to package nucleic acids into forms useful for therapeutic applications. Here we report that the two products of transcription, RNA and inorganic pyrophosphate, can self‐assemble form composite microsponge structures composed nanocrystalline magnesium pyrophosphate sheets (Mg 2 P O 7 •3.5H O) with adsorbed their surfaces. The particles contain high loadings (15–21 wt.%) are protected from degradation be obtained through a rolling circle...
Scalable methods, PRINT particle fabrication, and spray-assisted Layer-by-Layer deposition are combined to generate uniform functional nanotechnologies with precise control over composition, size, shape, surface functionality. A modular tunable approach towards design of built-to-order nanoparticle systems, spray coating on particles is demonstrated achieve technologies capable targeted interactions cancer cells for applications in drug delivery.
Purpose: Mutation of the Kirsten ras sarcoma viral oncogene homolog (KRAS) and loss p53 function are commonly seen in patients with non-small cell lung cancer (NSCLC). Combining therapeutics targeting these tumor-defensive pathways cisplatin a single-nanoparticle platform rarely developed clinic.Experimental Design: Cisplatin was encapsulated liposomes, which multiple polyelectrolyte layers, including siKRAS miR-34a were built on to generate multifunctional layer-by-layer nanoparticle....
Herein we report the potential of click chemistry-modified polypeptide-based block copolymers for facile fabrication pH-sensitive nanoscale drug delivery systems. PEG–polypeptide with pendant amine chains were synthesized by combining N-carboxyanhydride-based ring-opening polymerization post-functionalization using azide–alkyne cycloaddition. The contain a polypeptide amine-functional side groups and found to self-assemble into stable polymersomes disassemble in pH-responsive manner under...
Current treatment options for debilitating bone diseases such as osteosarcoma, osteoporosis, and metastatic cancer are suboptimal have low efficacy. New these pathologies require targeted therapy that maximizes exposure to the diseased tissue minimizes off‐target side effects. This work investigates an approach generating functional drug carriers specifically treating primary a disease in which recurrence is common cure rate has remained around 20%. utilizes modularity of Layer‐by‐Layer...
In biological fluids nanoparticles bind a range of molecules, particularly proteins, on their surface. The resulting protein corona influences activity and fate nanoparticle in vivo. Corona composition is often determined by the milieu encountered at entry portal into body, and, can therefore, depend route exposure to nanoparticle. For environmental where inhalation, this will be lung lining fluid. This study examined plasma bronchoalveolar fluid (BALF) binding engineered nanoparticles. We...
The construction of nanostructures from biodegradable precursors and shell/core crosslinking have been pursued as strategies to solve the problems toxicity limited stability, respectively. Polyphosphoester (PPE)-based micelles crosslinked nanoparticles with non-ionic, anionic, cationic zwitterionic surface characteristics for potential packaging delivery therapeutic diagnostic agents, were constructed using a quick efficient synthetic strategy importantly, demonstrated remarkable differences...
Methuosis is a novel nonapoptotic mode of cell death characterized by vacuole accumulation in the cytoplasm. In this article, we describe series azaindole-based compounds that cause vacuolization MDA-MB-231 cells. The most potent inducer, compound 13 (compound 13), displayed differential cytotoxicities against broad panel cancer lines, such as MDA-MB-231, A375, HCT116, and MCF-7, but it did not inhibit growth nontumorigenic epithelial line MCF-10A. A mechanism study confirmed was caused...
Chemical investigation on the South China Sea gorgonian coral Subergorgia suberosa has led to isolation of one new butenolide (5R)-5-(1-ethoxypropyl)-5-hydroxy-3,4-dimethylfuran-2(5H)-one (1) as a pair inseparable epimers, along with two known butenolides (S)-5-hydroxy-3,4-dimethyl-5-propylfuran-2(5H)-one (2) and (S)-5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one (3). Their structures including absolute configurations were determined unambiguously by detailed analyses spectroscopic data...
Approximately 80% of breast cancers overexpress the kinase tumor (BRK)/protein tyrosine 6, which has various oncogenic roles in cancer cell proliferation, survival, and migration. However, BRK inhibitors have yet to be explored as possible therapeutic tools. In this study, we used a parallel compound-centric approach discover new class pharmaceutical agents, exemplified by XMU-MP-2, potent selective inhibitors. XMU-MP-2 exhibited target-specific inhibition activity disrupted signaling...
Bruton's tyrosine kinase (BTK) plays a key role in B-cell receptor signalling by regulating cell proliferation and survival various malignancies. Covalent low-MW BTK inhibitors have shown impressive clinical efficacy However, the mutant Btk
Humans are exposed to nanoparticles in the environment as well those nanomaterials developed for biomedical applications. However, safety and biologic effects of many remain be elucidated. Over past decade, our understanding interaction proteins with various has grown. The protein corona can determine not only how interact cells but also their toxicity. In this study, we describe that several different classes exert on enzymatic activity cytosolic human arylamine <i>N</i>-acetyltransferase 1...