A5026368207- Veterinary Pharmacology and Anesthesia
- Animal testing and alternatives
- Organ Transplantation Techniques and Outcomes
- Animal Behavior and Welfare Studies
- Organ Donation and Transplantation
- Liver Disease Diagnosis and Treatment
- Advanced Chemical Sensor Technologies
- Stress Responses and Cortisol
- Adenosine and Purinergic Signaling
- Renal Transplantation Outcomes and Treatments
- Radiomics and Machine Learning in Medical Imaging
- Cardiac Arrest and Resuscitation
- Metabolomics and Mass Spectrometry Studies
- Themes in Literature Analysis
- Dietary Effects on Health
- Effects of Environmental Stressors on Livestock
- Physiological and biochemical adaptations
- Drug-Induced Hepatotoxicity and Protection
- Pain Mechanisms and Treatments
- Liver physiology and pathology
- Xenotransplantation and immune response
- bioluminescence and chemiluminescence research
- Pharmacological Receptor Mechanisms and Effects
- Ion Channels and Receptors
- Veterinary Orthopedics and Neurology
RWTH Aachen University
2017-2024
Universitätsklinikum Aachen
2022-2024
International University
2021
San Francisco Unified School District
2016
Rhodes University
2010
University of Hohenheim
2010
In many animal experiments scientists and local authorities define a body-weight reduction of 20% or more as severe suffering thereby potential parameter for humane endpoint decisions. this study, we evaluated distinct in multiple research facilities, assessed whether is valid criterion rodents. most (restraint stress, models epilepsy, pancreatic resection, liver caloric restrictive feeding mouse model Dravet syndrome) the animals lost less than their original body weight. glioma model, fast...
Oxidized and cross-linked modified proteins are known to accumulate in ageing. Little is about whether the accumulation of by advanced glycation end products (AGEs) due an affected intracellular degradation. Therefore, this study was designed determine enzymes cathepsin B, D 20S proteasome able degrade AGE-modified vitro. It shows that albumin degraded D, while B less effective degradation aldehyde-modified completely unable them. Mouse primary embryonic fibroblasts isolated from a knockout...
<b><i>Background:</i></b> The pig is one of the most frequently used large animal models for biomedical research, especially in field translational research and surgical models. While standard livestock breeds are short-term acute studies, minipig preferred long-term chronic studies due to their limited growth body weight. <b><i>Objective:</i></b> In consideration 3R principle (refinement, reduction, replacement) increasing demand, aim this...
Tissue adhesives constitute a great possibility to improve conventional wound closure. In contrast sutures, they enable nearly immediate hemostasis and can prevent fluid or air leaks. the present study, poly(ester)urethane-based adhesive was investigated which already proved be suitable for different indications, such as reinforcing vascular anastomosis sealing liver tissue. Using in vitro vivo setups, degradation of monitored over period up 2 years, evaluate long-term biocompatibility...
This paper reports on the farming and trade of lesser known crops, here termed African indigenous vegetables (AIVs), in Durban metropole. Most households grow AIVs, collect them from wild, primarily for home consumption. Modal income sale was approximately R30 per month farmer, most whom were middle-aged to elderly females, with limited education, who had been cultivating AIVs many years. The main constraints greater sales deemed be low market demand adverse climate. commonest grown pumpkin...
The Mouse Grimace Scale (MGS) has been widely used for the noninvasive examination of distress/pain in mice. aim this study was to further improve its performance generate repeatable, faster, blinded and reliable results developing automated standardized pictures MGS scoring simultaneous evaluation up four animals. Videos seven C57BL/6N mice were generated an experiment assess pain stress induced by repeated intraperitoneal injection carbon tetrachloride (CCl4). scores taken 1 h before after...
Abstract The Directive 2010/63 EU requires classifying burden and severity in all procedures using laboratory animals. This study evaluated the of liver fibrosis induction by intraperitoneal carbon tetrachloride (CCl 4 ) injections mice. 29 male C57BL/6N mice were treated three times per week for weeks with an injection (50 µl) either 0.6 ml/kg body weight CCl -vehicle solution, germ oil (vehicle-control) or handling only. Severity assessment was performed serum analysis, behavioral tests...
The Mouse Grimace Scale (MGS) is an established method for estimating pain in mice during animal studies. Recently, improved and standardized MGS set-up algorithm automated blinded output of images evaluation were introduced. present study evaluated the application this robustness associated at four facilities different locations as part varied experimental projects. Experiments using performed (F1-F4) included study; 200 pictures per facility (100 each rated positive negative by algorithm)...
Introduction: The P2X3 receptor (P2X3R), an ATP-gated non-selective cation channel of the P2X family, is expressed in sensory neurons and involved nociception. P2X3R inhibition was shown to reduce chronic neuropathic pain. In a previous screening 2000 approved drugs, natural products, bioactive substances, various non-steroidal anti-inflammatory drugs (NSAIDs) were found inhibit P2X3R-mediated currents. Methods: To investigate whether receptors contributes analgesic effect NSAIDs, we...
The aim of this pilot study was to evaluate whether behavioral or locomotor tests (Open Field (OF), rotarod (RR), and CatWalk (CW)) can help assess the severity laparotomy in rats. new EU Directive (2010/63/EU) mandates assessment experiments involving animals. However, validated objective methods are needed relate trial-specific monitoring results degree distress caused individual Therefore, we focused on non-invasive minimally invasive, simple, convenient a surgical model. To model,...
Abstract Human dermal sleeping nociceptors display ongoing activity in neuropathic pain, affecting 10% of the population. Despite advances rodents, a molecular marker for these mechano-insensitive C-fibers (CMis) human skin remains elusive, preventing targeted therapy. In this translational Patch-seq study, we combine single-cell transcriptomics following electrophysiological characterization with single-nucleus and spatial from pigs humans. We functionally identified CMis pig sensory...
Normothermic machine perfusion (NMP) of kidneys in combination with an optimized perfusate composition may increase donor organ preservation quality, especially the case marginal grafts. Optimization currently employed perfusates is still a subject present research. Due to advantages being cell-free, easy store, and having minimal antigenicity, hemoglobin-based oxygen carriers, such as HBOC-301 (Oxyglobin®, Hemoglobin Oxygen Therapeutics LLC, Souderton, PA, USA), offer alternative commonly...
The Next Generation Science Standards and the Common Core State for English Language Arts Literacy in have ignited interest developing novel ways to support students' literacy development science classes. To contribute these efforts, we developed WordSift, a free web-based vocabulary tool, that has series of interactive displays, including list 50 most frequent words particular text, Google image results any combination those words, definitions synonyms from context which appeared text....
Since the embedding of principles 3Rs (Replacement, Reduction and Refinement) in national international regulations on use animals, scientists have been challenged to find ways reduce number animals their research. Here, we present a digital platform, called '3R Backboard', linked laboratory animal management system, which facilitates sharing surplus biological materials from (e.g. tissues, organs cells) other research teams. Based information provided, such as genotype, age sex, workers...
Abstract Despite its long establishment and applicability in mice pain detection, the Mouse Grimace Scale still seems to be underused acute detection during chronic experiments. However, broadening can identify possible refinement approaches such as cumulative severity habituation painful stimuli. Therefore, this study focuses on two main aspects: First, five composite MGS criteria were evaluated with independent methods (the MoBPs algorithm a penalized least squares regression) ranked for...
Article 23(2) of EU Directive 2010/63 on the protection animals used for scientific purposes requires staff involved in care and use to be adequately educated trained before carrying out procedures. Therefore, 3Rs (refinement, reduction, replacement) knowledge alternative methods should part education training itself. For this purpose, digital learning concept "Virtual Reality (VR) Biomedical Education" evolved, which successfully combines VR components with classical content. Procedures,...
Ischemia-reperfusion injury remains a fundamental problem during organ transplantation logistics. One key technical factor is the rapid allograft rewarming time of vascular reconstruction in recipient. In this pilot study, new thermal insulation bag (TIB) for was used. Insulation capacity, tissue compatibility, and usability were tested initially ex vivo on porcine kidneys (n = 24) followed by first usage. Fourteen female German landrace pigs underwent kidney auto-transplantation after 24 h...
Laboratory animals used for experiments need to be monitored closely signs of pain and disstress. A well-established score is the mouse grimace scale (MGS), a method where defined morphological changes rodent's eyes, ears, nose, whiskers cheeks are assessed by human experts. While proven highly reliable, MGS assessment time-consuming task requiring manual processing videos key frame extraction subsequent expert grading. several tools have been presented support this white laboratory rats, no...
Severity assessment in biomedical research is required by the European authorities. Therefore, a variety of score sheets are available. The first were designed and introduced Morton Griffith (M&G) 1985, to assess pain distress animals. Score an important part 3R principles evaluate degree severity different studies. Here, we used modified sheet from M&G for 12 Aachen minipigs after partial liver resection safety testing novel synthetic sealant (VIVO-107). control group was treated...
Analysis of laboratory animal behavior allows assessment wellbeing. We present a method for the classification different activities mice by analyzing video clips using three deep learning methods. Animals placed in observation cages are filmed and short labelled as belonging to one five defined behaviors. Subsequently, methods based on convolutional neural networks (CNNS) applied classify clips. The best performing - two-stream network that analyzes individual frames well video's optical...
This study assessed the postoperative severity after three different visceral surgical interventions in rats by using objective parameters pertaining to various disciplines. The was evaluate whether degree of increases with invasiveness intervention and this is accordance EU Directive 2010/63. 136 adult male WistarHan were assigned groups: Sham-laparotomy (Sham) [7 days post-surgical survival time]; 50% partial hepatectomy (PH); 70% PH [PH groups 1, 3, or 7 times]. Post-surgical assessment...
Donor organ-shortage has resulted in the increased use of marginal grafts; however, normothermic machine perfusion (NMP) holds potential for organ viability assessment and restoration grafts prior to transplantation. Additionally, cell-, oxygen carrier-free antioxidants-supplemented solutions could potentially prevent adverse effects (transfusion reactions, inflammation, hemolysis), associated with autologous packed red blood cell (pRBC)-based perfusates. This study compared 6 h NMP porcine...