A5065256130- Pain Mechanisms and Treatments
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Neuropeptides and Animal Physiology
- Exercise and Physiological Responses
- Stress Responses and Cortisol
- Osteoarthritis Treatment and Mechanisms
- Neuroendocrine regulation and behavior
- HER2/EGFR in Cancer Research
- Rheumatoid Arthritis Research and Therapies
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Inflammatory mediators and NSAID effects
- Anesthesia and Pain Management
- Vagus Nerve Stimulation Research
- Peripheral Neuropathies and Disorders
- Musculoskeletal pain and rehabilitation
- Olfactory and Sensory Function Studies
- Monoclonal and Polyclonal Antibodies Research
- Neurogenesis and neuroplasticity mechanisms
- Bone health and treatments
- Myofascial pain diagnosis and treatment
- Neuroscience and Neuropharmacology Research
- Neurogenetic and Muscular Disorders Research
- Viral gastroenteritis research and epidemiology
- Retinoids in leukemia and cellular processes
Karolinska Institutet
2019-2025
University of California, San Diego
2025
Wake Forest University
2015-2021
Universidad Nacional Autónoma de México
2018
Gunma University
2015
Autonomous University of Tamaulipas
2014
Central University of Venezuela
1990
Fibromyalgia syndrome (FMS) is characterized by widespread pain and tenderness, patients typically experience fatigue emotional distress. The etiology pathophysiology of fibromyalgia are not fully explained there no effective drug treatments. Here we show that IgG from FMS produced sensory hypersensitivity sensitizing nociceptive neurons. Mice treated with displayed increased sensitivity to noxious mechanical cold stimulation, fibers in skin-nerve preparations mice an responsiveness...
Rheumatoid arthritis-associated joint pain is frequently observed independent of disease activity, suggesting unidentified mechanisms. We demonstrate that antibodies binding to cartilage, specific for collagen type II (CII) or cartilage oligomeric matrix protein (COMP), elicit mechanical hypersensitivity in mice, uncoupled from visual, histological and molecular indications inflammation. Cartilage antibody-induced pain-like behavior does not depend on complement activation inflammation, but...
Transferring fibromyalgia patient immunoglobulin G (IgG) to mice induces pain-like behaviour, and IgG binds mouse human satellite glia cells (SGCs). These findings suggest that autoantibodies could be part of pathology. However, it is unknown how frequently patients have anti-SGC antibodies associate with disease severity. Here, we quantified serum or plasma levels in 2 cohorts from Sweden Canada using an indirect immunofluorescence murine cell culture assay. Fibromyalgia binding SGCs dorsal...
Abstract High mobility group box 1 protein (HMGB1) is increasingly regarded as an important player in the spinal regulation of chronic pain. Although it has been reported that HMGB1 induces glial activation a Toll-like receptor (TLR)4-dependent fashion, aspect sexual dimorphisms not thoroughly addressed. Here, we examined whether action TLR4-activating, partially reduced disulfide on microglia nociceptive behaviors sex-dependent manner. We found to equally increase microglial Iba1...
A significant portion of adolescents suffer from mental illnesses and persistent pain due to repeated stress. It remains unclear which components the nervous system are involved in linkage between stress at an early age. Prior studies adult mice implicated innate immune system, specifically Toll-like receptors (TLR), as critical for inducing long-term anxiety pain-like behavior social defeat (SDS) model. In this work, we investigated behavioral phenotypes wild-type TLR4-deficient juvenile...
Many rheumatoid arthritis (RA) patients continue to experience persistent pain even after successful management of joint inflammation. Clinical data indicate that RA treated with the JAK inhibitor baricitinib consistently achieve relief cannot be entirely attributed its anti-inflammatory effects. In this study, we investigated antinociceptive properties using collagen antibody-induced (CAIA) model in which mechanical hypersensitivity persists long resolution The effects baricitinib,...
A significant portion of adolescents suffer from mental illnesses and persistent pain due to repeated stress. The components the nervous system that link stress in early life remain unclear. Prior studies adult mice implicated innate immune system, specifically Toll-like receptors (TLRs), as critical for inducing long-term anxiety pain-like behaviors social defeat (SDS) models. In this work, we investigated behavioral phenotypes wild-type TLR4-deficient juvenile subjected SDS evaluated...
Abstract Background: Patients with neuropathic pain show reduced endogenous analgesia induced by a conditioned noxious stimulus. Here, the authors tested whether peripheral nerve injury impairs descending noradrenergic inhibition from locus coeruleus (LC) after L5–L6 spinal ligation (SNL) in rats. Methods: A subdermal injection of capsaicin was used to examine stimulation–induced (NSIA), evoked LC glutamate and noradrenaline release, neuronal activity normal SNL The also examined role...
Abstract Introduction Clinical and preclinical studies have shown that supplementation with ω-3 polyunsaturated fatty acids (ω-3 PUFAs) reduce joint destruction inflammation present in rheumatoid arthritis (RA). However, the effects of individual PUFAs on chronic arthritic pain not been evaluated to date. Thus, our aim this study was examine whether purified docosahexaenoic acid (DHA, an PUFA) reduces spontaneous pain-related behavior knee edema improves functional outcomes a mouse model...
Abstract Recent studies in neuron‐glial metabolic coupling have shown that, the CNS, astrocytes and oligodendrocytes support neurons with energy‐rich lactate/pyruvate via monocarboxylate transporters (MCTs). The presence of such PNS, both Schwann cells neurons, has prompted us to question if a similar interaction may be present. Here we describe generation characterization conditional knockout mouse models where MCT1 or MCT4 is specifically deleted (named cKO). We show that cKO mice develop...
Abstract The pathophysiology of fibromyalgia syndrome (FMS) remains elusive, leading to a lack objective diagnostic criteria and targeted treatment. We globally evaluated immune system changes in FMS by conducting multiparametric flow cytometry analyses peripheral blood mononuclear cells identified natural killer (NK) cell decrease patients with FMS. Circulating NK were exhausted yet activated, evidenced lower surface expression CD16, CD96, CD226 more CD107a TIGIT. These hyperresponsive,...
Abstract Preclinical Research This study was performed to evaluate whether early, middle, or late treatment of zoledronate, an approved bisphosphonate that blocks bone resorption, can reduce nociceptive behaviors in a mouse arthritis model. Arthritis produced by repeated intra‐articular knee injections complete Freund's adjuvant (CFA). A dose‐response curve with zoledronate (3, 30, 100, and 300 μg/kg, i.p., day 4 25, twice weekly for 3 weeks) performed, the most effective dose (100 i.p.)...
Beta 2 adrenergic receptor (β2 AR) activation in the central and peripheral nervous system has been implicated nociceptive processing acute chronic pain settings with anti-inflammatory anti-allodynic effects of β2-AR mimetics reported several states. In current study, we examined therapeutic efficacy agonist clenbuterol a rat model persistent postsurgical hypersensitivity induced by disruption descending noradrenergic signaling rats plantar incision. We used growth curve modeling ipsilateral...
Background: Amyloid beta inhibits olfactory bulb function. The mechanisms involved in this effect must include alterations network excitability, inflammation and the activation of different transduction pathways. Thus, here we tested whether tolfenamic acid, a drug that modulates several these pathological processes, could prevent amyloid beta-induced dysfunction. Objective: To test acid prevents function, olfaction GSK3β activity. Method: protective effects against population activity...
Objective Transferring fibromyalgia patient IgG to mice induces pain-like behaviour and binds mouse human satellite glia cells (SGCs). These findings suggest that autoantibodies could be part of pathology. However, it is unknown how frequently patients have anti-SGC antibodies associate with disease severity. Methods We quantified serum or plasma levels in two cohorts from Sweden Canada using an indirect immunofluorescence murine cell culture assay. Fibromyalgia binding SGCs dorsal root...
SUMMARY Fibromyalgia syndrome (FMS) is a chronic pain condition characterized by widespread and tenderness 1,2 . The etiology pathophysiology of fibromyalgia are unknown there no effective treatments. Here we show that sensory hypersensitivity in FMS caused autoantibodies act sensitizing nociceptive neurons. Administration IgG from patients increased mouse sensitivities to stimulation with mechanical pressure cold. In contrast, transfer depleted samples or healthy control subjects had effect...
Nerve growth factor (NGF)-R100E is a mutated form of human recombinant NGF that reduces the binding to its p75NTR receptor while retaining affinity toward TrkA receptor. Here, we used wild type and NGF-R100E knock-in mice investigate effects this mutation on inflammation-induced pain-related behaviors bone loss. The hNGF-R100E did not alter nerve fiber density in sciatic nerve, ankle joint synovium, skin naïve mice. Withdrawal responses mechanical, thermal, cold stimuli before after...
Bacterial infection results in a veritable cascade of host responses, both local and systemic. To study the initial stages host-pathogen interaction living tissue we use spatially-temporally controlled vivo models. Using this approach, show here that within 4 h uropathogenic Escherichia coli (UPEC) kidney, an IFNγ response is triggered spleen. This rapid infection-mediated inter-organ communication was found to be transmitted via nerve signalling. expression toxin α-hemolysin directly...