A5019094179- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- Vaccine Coverage and Hesitancy
- HIV Research and Treatment
- Influenza Virus Research Studies
- Congenital Anomalies and Fetal Surgery
- Immune responses and vaccinations
- Hepatitis Viruses Studies and Epidemiology
- COVID-19 Clinical Research Studies
- Infant Nutrition and Health
- HIV/AIDS Research and Interventions
- Virology and Viral Diseases
- HIV/AIDS drug development and treatment
- vaccines and immunoinformatics approaches
- Vector-Borne Animal Diseases
- Escherichia coli research studies
- Pneumonia and Respiratory Infections
- Pneumocystis jirovecii pneumonia detection and treatment
- Immune Response and Inflammation
- COVID-19 Impact on Reproduction
- Clostridium difficile and Clostridium perfringens research
Program for Appropriate Technology in Health
2015-2024
Seattle University
2015-2020
Science Research Laboratory
2020
Research Research (South Africa)
2020
Clinical Research Organization
2020
Czech Academy of Sciences, Institute of Experimental Medicine
2015
Office of AIDS Research
2000-2012
National Institute of Allergy and Infectious Diseases
1990-2009
National Institutes of Health
1991-2009
Emory University
2008
Five genetically distinct human rotavirus (HRV) gene 4 groups have been described on the basis of comparative nucleotide sequencing and predicted amino acid sequences, at least four them represent VP4 antigenic types. To identify each type investigate its distribution in HRV isolates from patients with diarrhea, we developed a polymerase chain reaction (PCR) typing method using sequence information available for Rotavirus double-stranded RNAs (dsRNAs) isolated stool samples were first...
Antiserum prepared against the M37 strain of rotavirus, recovered from an asymptomatic newborn infant in Venezuela, neutralized two prototype human rotaviruses that define separate serotypes: serotype 1 (Wa) and 4 (ST3). Thus, is a naturally occurring intertypic rotavirus. Analysis reassortant viruses produced during coinfection vitro indicated observed dual specificity resulted sharing related outer capsid protein, VP3, with ST3 virus another VP7, Wa virus. single (VP3)-gene-substitution...
Ten monoclones directed to the 42,000-dalton inner structural protein of rotavirus were analyzed. Eight reacted broadly with antigenic domains common virtually all mammalian rotaviruses. Two had specificities similar or identical previously characterized subgroup specificities. These more efficient in detecting antigen than either hyperimmune postinfection antisera. Using monoclones, we determined that some animal as well human strains carry 2 specificity and epizootic diarrhea infant mice...
A series of monoclonal antibodies was isolated which reacted with one two major surface proteins rhesus rotavirus. Thirty-six immunoprecipitated the 82-kilodalton outer capsid protein, product fourth gene, viral hemagglutinin. These exhibited hemagglutination inhibition activity and neutralized rotavirus to moderate or high titer. Three 38-kilodalton glycoprotein, eighth ninth gene product. three titer also inhibited hemagglutination.
Of 73 rotavirus-positive fecal specimens tested, 39 yielded a human rotavirus that could be cultivated serially in MA104 or primary African green monkey kidney cells both; 18 were serotyped. Four distinct serotypes identified by plaque reduction tube neutralization assay both, and three of these the same as those established previously reduction, using rotaviruses genetic reassortment with cultivable bovine rotavirus. Ten strains received from Japan found to similar, if not identical, our...
Twenty different human rotavirus reassortants were characterized serologically by a plaque reduction assay as belonging to one of three distinct serotypes. Fourteen similar if not identical our prototype Wa strain; two like the DS-1 strain, and four belonged third serotype for which has yet been selected. Hyperimmune sera raised against serotypes required distinguish among them, since postinfection had lower titers more cross-reactive than hyperimmune sera. These results confirmed ability...
An immune adherence hemagglutination assay (IAHA) and a modified enzyme-linked immunosorbent for antigenic characterization of human rotaviruses were developed. The designations type 1 2 identical to those established previously by specific complement fixation, assay, electron microscopy. By IAHA (and assay) certain animal found be closely related rotavirus 1. pattern reactivity the cell culture neutralization serotype distinct properties. separation was apparent when which distinguishable...
Efficacy of live oral rotavirus vaccines is reduced in low-income compared with high-income settings. Parenteral non-replicating might offer benefits over vaccines. We assessed the safety and immunogenicity P2-VP8-P[8] subunit vaccine at different doses South African toddlers infants.This double-blind, randomised, placebo-controlled, dose-escalation trial was done a single research unit based hospital Africa healthy HIV-uninfected (aged 2 to <3 years) term infants 6 <8 weeks, without...
Rotavirus is the most common cause of moderate-to-severe infant diarrhoea in developing countries, resulting enormous morbidity, mortality, and economic burden. A bovine-human reassortant pentavalent rotavirus vaccine (BRV-PV) targeting globally strains was developed India tested a randomized, double-blind, placebo-controlled end-point driven Phase III efficacy clinical trial implemented at six sites across India. Infants 6 to 8 weeks age were randomized (1:1) receive three oral doses BRV-PV...
A monovalent, parenteral, subunit rotavirus vaccine was well tolerated and immunogenic in adults the USA toddlers infants South Africa, but elicited poor responses against heterotypic strains. We aimed to evaluate safety immunogenicity of a trivalent formulation (P2-VP8-P[4],[6],[8]).A double-blind, randomised, placebo-controlled, dose-escalation, phase 1/2 study done at three African research sites. Healthy (aged 18-45 years), 2-3 6-8 weeks, ≥37 weeks' gestation, without previous receipt...
Two new human rotavirus (HRV) strains, PA151 and PA169, with subgroup I specificity a long RNA pattern, yet serotype G (VP7) different from those of any the six well-established HRV serotypes (G1 to G4, G8, G9), were isolated 3 months apart two children acute gastroenteritis in Sicily, southern Italy, winter season 1987 1988. The isolates adapted growth cell cultures then characterized by neutralization RNA-RNA (Northern blot) hybridization. Cross-neutralization studies type-specific immune...
Live attenuated viral vectors that express human immunodeficiency virus (HIV) antigens are being developed as potential vaccines to prevent HIV infection. The first phase 2 trial with a canarypox vector (vCP205, which expresses gp120, p55, and protease) was conducted in 435 volunteers without gp120 boosting, expand the safety database compare immunogenicity of who were at higher risk lower for Neutralizing antibodies MN strain stimulated 94% given vCP205 plus 56% alone. CD8+ cytotoxic T...
RNA-RNA hybridization was performed to assess the extent of genetic relatedness among human rotaviruses isolated from children with gastroenteritis and asymptomatic newborn infants. 32P-labeled single-stranded RNAs produced by in vitro transcription viral cores different strains tested were used as probes two assays: undenatured genomic resolved polyacrylamide gel electrophoresis, denatured situ, electrophoretically transferred diazobenzyloxymethyl-paper (Northern blots), then hybridized...
Journal Article Protection of Peruvian Children Against Rotavirus Diarrhea Specific Serotypes by One, Two, or Three Doses the RIT 4237 Attenuated Bovine Vaccine Get access Claudio F. Lanata, Lanata Please address requests for reprints to Dr. Instituto de Investigacion Nutricional, Apartado 18-0191, Lima 18, Peru. Search other works this author on: Oxford Academic PubMed Google Scholar Robert E. Black, Black Roberto del Aguila, Aguila Ana Gil, Gil Hector Verastegui, Verastegui Guiseppe Gerna,...
The ribonucleic acid migration patterns of 7 subgroup 1 and 16 2 human rotaviruses recovered from four geographic areas were compared. had strikingly slower-moving segments 10 11, suggesting a correlation between the pattern specificity.
Nineteen rotavirus strains derived from asymptomatic neonates (seven England, five Australia, two Venezuela, and Sweden) were successfully cultivated in primary African green monkey kidney cell cultures, serotyped by plaque reduction neutralization tests, subgrouped indirect enzyme-linked immunosorbent assay, electropherotyped polyacrylamide gel electrophoresis. All 19 shown to fall into one of the four known human serotypes; serotype 1 (all Venezuelan strains), 2 Swedish 3 Australian or 4...
We have previously found (O. Nakagomi, T. H. Oyamada, and Suto, J. Med. Virol. 17:29-34, 1985), during an epidemiological study in Japan, a novel human rotavirus that belongs to subgroup I but has long RNA pattern typical of II rotaviruses. From the stool specimen containing this virus, we successfully isolated MA104 cells rotavirus, designated AU-1, which possesses these characteristics. The possibility strain AU-1 was laboratory contaminant animal adapted tissue culture ruled out, identity...
Within the past few years, rotavirus strains were recovered from four discrete prolonged outbreaks of infection in newborn nurseries which affected infants failed to develop significant symptoms. The virus each outbreak belonged a different human serotype and thus serotypes was associated with asymptomatic neonates. Marked conservation sequence observed among fourth genes nursery previous study using RNA X hybridization, while conserved set gene sequences identified virulent rotaviruses...
In April 2006, the National Institute of Allergy and Infectious Disease (NIAID)-funded HIV Vaccine Trials Network NIAID Division AIDS sponsored a workshop at which nonhuman primate (NHP) researchers clinical trial scientists with vaccine research expertise discussed how to more effectively use NHPs for evaluating HIV-1 candidates. This precipitated broad discussion on what types NHP studies should be targeted in critical preclinical pathway candidates, especially those designed elicit...