A5074392033- Retinal Development and Disorders
- Retinal and Macular Surgery
- Neuroscience and Neural Engineering
- Retinal Diseases and Treatments
- Cellular Mechanics and Interactions
- Virus-based gene therapy research
- Medical and Biological Sciences
- Mitochondrial Function and Pathology
- Pluripotent Stem Cells Research
- Barrier Structure and Function Studies
- RNA Interference and Gene Delivery
- Ocular Oncology and Treatments
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Glaucoma and retinal disorders
- Retinopathy of Prematurity Studies
- Genomics and Chromatin Dynamics
- Cancer-related Molecular Pathways
- Coenzyme Q10 studies and effects
Czech Academy of Sciences, Institute of Animal Physiology and Genetics
2021-2025
Charles University
2021-2025
Czech Academy of Sciences
2022
Oslo University Hospital
2022
University of Oslo
2022
Institute of Molecular Biology and Genetics
2019
Aims/Purpose: The study of long‐term survival transplanted retinal pigment epithelium (RPE) in animal models is very important for the development promising cell‐based therapy degenerative diseases such as age‐related macular degeneration (AMD) or retinitis pigmentosa. We hereby present xenogeneic implantation human iPSC‐RPEs into minipig eyes with a 6‐week follow‐up period. Methods: subretinal implantations hiPSC‐RPEs‐scaffolds were performed using 3‐port pars plana vitrectomy. character...
Purpose: This study aimed to evaluate early-phase safety of subretinal application AAVanc80.CAG.USH1Ca1 (OT_USH_101) in wild-type (WT) pigs, examining the effects a vehicle control, low dose, and high dose. Methods: Twelve WT pigs (24 eyes) were divided into three groups: four each received bilateral injections either vehicle, dose (3.3 × 1010 vector genomes [vg] per eye), or (1.0 1011 vg eye). Total retinal thickness (TRT) was evaluated using optical coherence tomography function assessed...
In late stages of inherited and acquired retinal diseases such as Stargardt disease (STGD) or dry age-related macular degeneration (AMD), loss pigment epithelia (RPE) cells subsequently photoreceptors in the area result a dramatic decline central visual function. Repopulating this with functional RPE may prevent progression photoreceptor loss. present study, viability, survival, integration human induced pluripotent stem cell (hiPSC)-derived (hiPSC-RPE) is assessed generated using...
The development of primary human retinal pigmented epithelium (hRPE) for clinical transplantation purposes on biodegradable scaffolds is indispensable. We hereby report the results subretinal implantation hRPE cells nanofibrous membranes in minipigs.The hRPEs were collected from cadaver donor eyes and cultivated ultrathin carriers prepared via electrospinning poly(L-lactide-co-DL-lactide) (PDLLA). "Libechov" minipigs (12-36 months old) used study, supported by preoperative tacrolimus...
Purpose To assess the safety and feasibility of direct vitrectomy-sparing subretinal injection for gene delivery in a large animal model.
Purpose: Retinal ischemia (RI) and progressive neuronal death are sight-threatening conditions. Mitochondrial (mt) dysfunction fusion/fission processes have been suggested to play a role in the pathophysiology of RI. This study focuses on changes mt parameters neuroretina, retinal pigment epithelium (RPE) choroid porcine high intraocular pressure (IOP)-induced RI minipig model. Methods: In one eye, an acute IOP elevation was induced minipigs compared other control eye. Activity amount...
Dysfunction of the retinal pigment epithelium (RPE) causes numerous forms degeneration. RPE replacement is a modern option to save vision. We aimed test results transplanting cultured RPEs on biocompatible membranes.We cultivated porcine primary cells isolated from cadaver eyes slaughterhouse two types membranes: commercial polyester scaffolds Transwell (Corning Inc., Kenneburg, ME, USA) with 0.4 µm pore size and prepared Poly (L-lactide-co-DL-lactide) (PDLLA) nanofibrous membranes an...
Purpose: Subretinal (SR) injection in porcine models is a promising avenue for preclinical evaluation of cell and gene therapies. Targeting the subretinal fluid compartment (bleb) critical to procedure, especially if treatment cone-rich area centralis required (i.e., visual streak [VS] pigs). To our knowledge, this study first investigate influence site placement on VS involvement pig eye. Methods: We performed 23-gauge pars plana vitrectomy followed by SR 41 eyes 21 animals (Sus scrofa...
Aim. Regardless a great progress in treatment, 15 % of breast cancer cases remain lethal.One the main problems diagnosis and cure this type is its high clinical genetic heterogeneity, identification markers for personalized treatment still topical issue.Methods.Collection characterization material, RNA isolation analysis ITSN2 TKS5 isoforms expression using real-time quantitative PCR with fluorescence labeled probes.Results.The reduced ITSN2-S has been found HER2/neupositive tumors poor...
Degenerative disorders of the retina (including age-related macular degeneration), which originate primarily at or within retinal pigmented epithelial (RPE) layer, lead to a progressive disorganization anatomy and deterioration visual function. The substitution damaged RPE cells (RPEs) with in vitro cultured using subretinal cell carrier has shown potential for re-establishing anatomical structure outer layers is, therefore, being further studied. Here, we present principles surgical...
Abstract The development of primary human retinal pigmented epithelium (hRPE) for clinical transplantation purposes on biodegradable scaffolds is indispensable. results the subretinal implantation hRPE cells nanofibrous membranes in minipigs are hereby reported. hRPEs were collected from cadaver donor eyes and cultivated ultrathin carriers prepared via electrospinning poly(L‐lactide‐co‐DL‐lactide) (PDLLA). “Libechov” (12–36 months old) used study, supported by preoperative tacrolimus...
Aim. TKS5 is a key scaffold protein of invadopodia. In its absence, the cells completely lose ability to form This fact makes potential target for cancer cure and one central proteins in investigation cell invasion. Additionally, question remains about function normal cells. Therefore, order extend knowledge role healthy invasive cells, we tested interaction with involved signal transduction: PLCγ1, SRC, CRK, CSK; plasma membrane remodeling: AMPH1, BIN1, CIN85, ITSN1, ITSN2; actin...
Degenerative disorders of the retina (including age-related macular degeneration), which originate primarily at or within retinal pigmented epithelial (RPE) layer, lead to a progressive disorganization anatomy and deterioration visual function. The substitution damaged RPE cells (RPEs) with in vitro cultured using subretinal cell carrier has shown potential for re-establishing anatomical structure outer layers is, therefore, being further studied. Here, we present principles surgical...
Retinoblastoma (Rb), the most common primary intraocular tumor in childhood, is specific its location inside eye globe and, more advanced disease, occurrence of vitreous seeds. Finding an effective treatment thus requires adequate animal model. As a large model has not yet been described, purpose this study was to create porcine Rb Eight male minipigs (8 eyes) without immunosuppression were used study. A suspension cultured WERI-Rb-1 cells, 100 µl with concentration 106 cells/ml, injected...