A5101554743- Research on Leishmaniasis Studies
- Parasites and Host Interactions
- Trypanosoma species research and implications
- Immune Cell Function and Interaction
- Macrophage Migration Inhibitory Factor
- Acute Lymphoblastic Leukemia research
- Cytokine Signaling Pathways and Interactions
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
- Celiac Disease Research and Management
- Plant Virus Research Studies
- Asthma and respiratory diseases
- Signaling Pathways in Disease
- Eosinophilic Disorders and Syndromes
- IL-33, ST2, and ILC Pathways
- Clostridium difficile and Clostridium perfringens research
- Cell Adhesion Molecules Research
- Sarcoma Diagnosis and Treatment
- Transgenic Plants and Applications
- Tryptophan and brain disorders
- Gut microbiota and health
- Invertebrate Immune Response Mechanisms
- Cancer, Stress, Anesthesia, and Immune Response
- Autoimmune and Inflammatory Disorders Research
- Lysosomal Storage Disorders Research
National Centre for Cell Science
2012-2024
University of Pittsburgh
2022-2024
Savitribai Phule Pune University
2020
Two different Toll-like receptors (TLRs) have been shown to play a role in host responses Leishmania infection. TLR-2 is involved parasite survival macrophages upon activation by lipophosphoglycan (LPG), virulence factor expressed Leishmania. In contrast, of TLR-9 has promote host-protective response. However, whether there relationship between the interaction LPG and TLR-2, on one hand, with effect TLR-9, other remains unknown. this study, we report that in-vitro infection L. major high...
TLRs recognize pathogen-expressed Ags and elicit host-protective immune response. Although TLR2 forms heterodimers with TLR1 or TLR6, recognizing different ligands, differences in the functions of these remain unknown. In this study, we report that Leishmania major-infected macrophages, expression TLR2, but not increased; TLR2-TLR2 association increased, TLR2-TLR6 diminished. Lentivirus-expressed TLR1-short hairpin RNA (shRNA) TLR2-shRNA administration reduced, TLR6-shRNA increased L. major...
Immunological homeostasis is often maintained by counteractive functions of two different cell types or receptors signaling through intermediates in the same cell. One these protein kinase C (PKC). Ten differentially regulated PKC isoforms are integral to receptor-triggered responses cells. So far, eight reported be expressed macrophages. Whether a single receptor uses regulate effector has never been addressed. As CD40 only characterized trigger functions, we examined relative role...
Summary Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns and results in innate immune system activation that elicitation of the adaptive response. One crucial modulator response is CD40. However, whether these molecules influence each other's expression functions not known. Therefore, we examined effects TLRs on CD40 macrophages, host cell for protozoan parasite Leishmania major. While polyinosinic-polycytidylic acid [poly (I:C)], a TLR-3 ligand, lipopolysaccharide...
Abstract Leishmania major is a parasite that resides and replicates in macrophages. We previously showed the enhanced CD40-induced Raf-MEK-ERK signaling but inhibited PI3K-MKK-p38MAPK to proleishmanial effects. As Raf PI3K have Ras-binding domain exert opposite effects on infection, we examined whether Ras isoforms had differential roles infection. observed L. N-Ras H-Ras expression K-Ras infection activity activity. TLR2 short hairpin RNA or anti-TLR2 anti-lipophosphoglycan Abs reversed...
Leishmania major causes cutaneous leishmaniasis. An antileishmanial vaccine for humans is unavailable. In this study, we report development of two attenuated L. strains-5ASKH-HP and LV39-HP-by continuous culture (high passage) the corresponding virulent strains (low passage). Both avirulent showed similar changes in proteome profiles when analyzed by surface-enhanced laser desorption ionization mass spectrometry. Liquid chromatography-mass spectrometry microarray characterization 5ASKH...
Multiple pathogen-associated molecular patterns (PAMPs) on a pathogen's surface imply their simultaneous recognition by the host cell membrane-located multiple PAMP-specific Toll-like receptors (TLRs). The TLRs endosomes recognize internalized pathogen-derived nucleic acids and trigger anti-pathogen immune responses aimed at eliminating intracellular pathogen. Whether influence each other's expression effector responses-termed TLR interdependency-remains unknown. Herein, we first probed...
Understanding the initiation of T-helper (Th)-2 immunity is crucial for addressing allergic diseases that have been linked to commensal microbiota. However, Th2 responses are notably absent from known host-microbiota intestinal immune circuits. Notably, protist
INTRODUCTION: Chordomas are rare tumors that arise from remnants of the notochord. The standard care for chordoma remains only surgery and radiotherapy. Though checkpoint inhibition therapy shows promise, recent immunotherapy trials failed to reach endpoints. This emphasizes need comprehensively understand tumor peripheral immune landscape chordomas establish a rationale immunotherapeutic intervention. METHODS: We analyzed quantified cell composition (CD3 + , CD4 CD8 CD20 CD163 cells) 33 (14...
Abstract Loss of oral tolerance (LOT) to gluten, characterized by a T helper 1 (Th1) gluten-specific immune response, is hallmark celiac disease (CeD) and can be triggered enteric viral infections. We hypothesized that certain gut microbes have the capacity protect against virus-mediated LOT. By using our previously defined reovirus-mediated LOT CeD model, we discovered colonizing protist Tritrichomonas (T.) arnold promotes protects suppressing reovirus-induced proinflammatory program...