A multimodal drug delivery system targeting the tumor microenvironment is an inspiring method for treating cancer tissues, including oral squamous cell carcinomas (OSCC).

To ensure site–specific drug release in tumor cells and cancer-associated fibroblasts reduce the systemic toxicity of chemotherapy, a novel delivery system called human serum albumin-indocyanine green-cisplatin nanoparticles was developed.

Abstract Background Local recurrence is the main cause of death among patients with oral squamous cell carcinoma (OSCC). This study assessed near-infrared fluorescence (NIF) imaging and spectroscopy to monitor surgical margins intraoperatively for OSCC. Methods Cytological animal experiments were first performed confirm feasibility monitoring NIF spectroscopy. Then, 20 OSCC included in clinical trials. At 6–8 h after 0.75 mg/kg indocyanine green (ICG) injection, all underwent surgery...

Objectives: Depth of invasion (DOI) in oral squamous cell carcinoma (OSCC) guides treatment and prognosis but lacks three-dimensional (3D) insight. Thus, this study aimed to investigate the feasibility accuracy Lugol's iodine-enhanced micro-computed tomography (CT) for 3D measurement DOI OSCC samples. Methodology: In total, 50 vitro samples from Nanjing Stomatological Hospital (July 2022 January 2024) were subjected micro-CT imaging with a slice thickness μm following 3% Lugol iodine...

The heterogeneity of cancer-associated fibroblasts (CAFs) in head and neck squamous cell carcinoma (HNSCC) has been widely acknowledged, but not yet elucidated. potential roles clinical relevance CAFs subclusters HNSCC progression remain obscure.In this study, we combined single-cell bulk tissue transcriptome profiles with data from Cancer Genome Atlas (TCGA). Seurat package was used to perform RNA-seq analysis distinguish distinct subtypes. Prognostic several markers assessed functional...

Abstract Background Tumor metastasis is responsible for the high mortality rate of patients with oral squamous cell carcinoma (OSCC). Although many hypotheses have been proposed to elucidate mechanism tumor metastasis, origin metastatic cells remains unclear. In this study, we explored role fusion in formation OSCC cells. Methods Murine and macrophages were fused vitro, proliferation, migration, phagocytosis abilities hybrid parental compared. Subsequently, compared transcriptome differences...

A novel thermally activated delayed fluorescence photosensitizer was designed for oral squamous cell carcinoma treatment, which effectively inhibited tumor growth under low intensity light with minimal damage to normal tissues.

In this study, a drug delivery system based on glutathione (GSH)-sensitive and folic acid (FA)-targeted nanoparticles loaded with paclitaxel (FA-PEG-S-S-PCL@PTX, FA-NPs) was developed.

Background: Accurate assessment of regional lymph node (LN) status is essential for the treatment head and neck squamous cell carcinoma (HNSCC) patients. In this study, we aimed to compare difference between intravenous injection indocyanine green (ICG) peritumoral ICG in location metastatic LNs.Methods: Twenty-nine patients were enrolled study with 13 receiving 16 ICG. During surgery, fluorescence-positive LNs vivo sent undergo frozen section after fluorescence intensity was recorded. After...

With the frequent use of Bisphosphonates (BPs), morbidity BP-related osteonecrosis jaw (BRONJ) is also increasing. However, prevention and treatment BRONJ faced with enormous challenges. This study aimed to illuminate influence BP administration in rat mandible explore feasibility discriminating lesion bone Raman spectroscopy.First, we explored time- mode-dependent effects on spectroscopy. Second, model was constructed, healthy components were analyzed using spectroscopy.When only BPs...

Abstract Oral cancer is a multifactorial that affects millions of peoples worldwide. The current exploration aimed to evaluate the mechanisms thymoquinone nanoencapsulated carrier and its effects on 7,12‐Dimethylbenz[a]anthracene (DMBA) stimulated hamster buccal pouch in Syrian model. Nanocarrier was characterized by SEM, TEM, FTIR analysis. incidence tumor, biochemicals makers studied through standard methods. mRNA expression level inflammatory markers NF‐κBp50, NF‐κBp65, PI3K/AKT/mTOR...

A positive surgical margin (PSM) following oral cancer resection results in local recurrence and poor prognosis. Mono-block tumor specimens, especially from the base, are difficult to evaluate. This inaccurate sampling ultimately leads a false pathological diagnosis. Lugol's iodine (I2-IK)-enhanced micro-CT is an emerging method image specimens. study explores feasibility of I2-IK-enhanced evaluate for tongue squamous cell carcinoma (TSCC) specimens further seek optimal staining...

Recent advances in circulating microRNAs (miRNAs) as noninvasive biomarkers have provided promising prospect detecting colorectal cancer (CRC). However, the capability of miRNAs for neoplasia (CRN, including precancerous lesions and curable stage CRCs) remains unclear. This study aimed to identify potential serum (miR-20a, miR-486, miR-92a, miR-135b) selected from literature discriminating CRN patients.The samples 46 patients 33 healthy controls were analyzed with quantitative reverse...

Gold nanoparticles (GNPs) exhibited unprecedented size dependent anti-CAF potential, wherein the smallest GNPs outperformed their larger counterparts.

Hypoxic and acidic environments are the two main components of microenvironment contributing to poor efficacy chemotherapy drugs in treatment oral squamous cell carcinoma (OSCC). In this study, we synthesized a series Zn1-x Mg x Fe2O4 nanomaterials with enzyme-like properties, including catalase (CAT)-like, peroxidase (POD)-like, glutathione (GSH)-like activity an environment. Among them, Zn0.4Mg0.6Fe2O4 performed best effectively increased doxorubicin (DOX) for OSCC reduced cardiotoxicity....

Herein, we reported nitroxide radical-modified CuS nanoparticles (CuS-NO˙ NPs), and they exhibited a typical absorption peak at 1182 nm. Due to such long wavelength absorbance, CuS-NO˙ NPs excellent therapeutic outcome low damage normal tissues. Besides, simultaneously achieved for MRI CT dual-modal imaging, which successfully provided new strategy imaging-guided tumor treatment, thus increasing potential clinical applications cancer treatment.

Abstract Desmoplasia in fibroblasts within metastatic lymph nodes (MLNs) serves as an indicator of extranodal extension (ENE), which is a critical determinant distant metastasis and mortality oral squamous cell carcinoma (OSCC). However, systematic studies on MLNs are lacking. Therefore, this study investigated characterized the differences phenotype, function, origin between primary tumors (PTs) (LNs) OSCC. We generated single-cell maps PTs paired draining LNs from three OSCC patients....

Abstract The morbidity of bisphosphonate‐related osteonecrosis the jaw (BRONJ) is also increasing with use bisphosphonates (BPs). Removing affected bone accurately most effective treatment. This study aimed to explore feasibility Indocyanine green (ICG)‐based Near‐Infrared fluorescence (NIF) imaging remove BRONJ bone. Firstly, rat model was constructed. And 5 mg/kg ICG were injected via tail vein, after 12 hr, and healthy dissected for ICG‐based NIF quantification detection intensity....

Abstract Background Local recurrence is the main cause of death among patients with oral squamous cell carcinoma (OSCC). This study assessed near-infrared fluorescence (NIF) imaging and spectroscopy to monitor surgical margins intraoperatively for OSCC. Methods Cytological animal experiments were first performed confirm feasibility monitoring NIF spectroscopy. Then, 20 OSCC included in clinical trials. At 6–8 hours after 0.75 mg/kg indocyanine green (ICG) injection, all underwent surgery...

Accurate evaluation of lymph node (LN) status is the key factor to determine treatment and evaluate prognosis for patients with cancer. However, traditional pathological examination resulted in a 30% false-negative rate detection metastases LNs. This study aimed utilize Lugol's iodine (I2-IK)-enhanced micro-CT imaging reveal 3-dimensional structure regional LNs decrease examination.To explore feasibility I2-IK-enhanced locating metastatic lesion LNs, nonmetastatic from mice were used mimic...

Abstract Purpose Depth of invasion (DOI) in oral squamous cell carcinoma (OSCC) guides treatment and prognosis but lacks three-dimensional (3D) insight. We used Lugol’s iodine-enhanced micro-computed tomography (CT) for 3D analysis. Methods Fifteen vitro OSCC samples from Nanjing Stomatological Hospital (July to December 2022) were micro-CT imaged with a slice thickness 50 µm following 3% iodine staining 12 h then pathologically sectioned stained. The panoramic diagnostic scanner measured...