Recently, more and evidence are rapidly accumulating that long noncoding RNAs (lncRNAs) involved in human tumorigenesis misregulated many cancers, including colon cancer. LncRNA could regulate essential pathways contribute to tumor initiation progression with their tissue specificity, which indicates lncRNA would be valuable biomarkers therapeutic targets. Colon cancer-associated transcript 1 (CCAT1) is a 2628 nucleotide-lncRNA located the vicinity of well-known transcription factor c-Myc....

This study aims to screen microRNAs (miRNAs), for an early diagnosis of colorectal cancer, by deep sequencing and evaluation total miRNAs using clinical samples from a Chinese patient population.Total small RNAs normal colonic mucosa, adenomas, cancer tissues were prepared miRNA analysis sequencing. The data then analyzed bioinformatics candidate diagnostic miRNAs, which further validated their up- or downregulation status.Comparison with mucosa identified 99 upregulated 90 downregulated...

Globally, liver cancer poses a serious threat to human health and quality of life. Despite numerous studies on the microbial composition gut in hepatocellular carcinoma (HCC), little is known about interactions microbiota metabolites their role HCC. This study examined serum metabolic profiles 68 patients with HCC, 33 cirrhosis (LC), 34 healthy individuals (NC) using combination metagenome sequencing liquid chromatography−mass spectrometry (LC−MS). The structure intestinal were found be...

Aims: To explore the inhibitory effects of epigallocatechin gallate (EGCG) on proliferation colorectal cancer cells and gene expression Notch signaling. Methods: The orthotopic transplant model were treated with EGCG, MTT assay was used to test role EGCG in cells. Results: indicated that inhibited these four cell lines when time concentration increased, enhanced apoptotic rate lines. dosage positively correlated rate, by influencing cycle. In-vivo study suggested seventh day, volume tumors...

Objective: To investigate the risk of colorectal cancer and its relationship with colonic flora microenvironment under high-fat high-calorie diet.Methods: Wistar rats were used to study, they given normal diet, dimethyl hydrazine (DMH) induce occurrence cancer.Then observe difference in tumor formation among microbial community, inflammatory factors metabolism.Results: No tumors found diet group (G1) (G3).Four nodules four + DMH (G2) 8 cancerous formed 7 (70%) from (G4).Cholesterol TNF-α...

Hereditary nonpolyposis colorectal cancer (HNPCC) is an autosomal dominant syndrome. The National Cancer Institute (NCI) has recommended the Revised Bethesda guidelines for screening HNPCC. There been a great deal of research on value these tests in other countries. However, literature about Chinese population scarce. Our objective to detect and study microsatellite instability (MSI) mismatch repair (MMR) gene germline mutation carriers among with cancer. In 146 prospectively recruited...

Oridonin is the active ingredient isolated from Chinese herb Rabdosia rubescens. We used both in vivo and vitro approaches to elucidate underlying mechanism of oridonin-mediated inhibition colorectal cancer.Two cell lines, Lovo SW480, were treated with oridonin solution. The effect this treatment on proliferation rate was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. changes gene expression that occurred lines response via an illumine sensor....

To established a procedure for laparoscopic extraperitoneal ostomy after abdomino-perineal resection (APR) and study safety aspects complications.From July 2011 to 2012, 36 patients with low rectal cancer undergoing APR were included in the divided into group (n = 18) intraperitoneal 18). Short- long-term complications compared between two groups. All followed up median duration was 17 months (range: 12-24).The rates of short-term complication related colostomies comparable groups, except...

AIM:To determine the accuracy of endoscopic polyp size measurements using disposable graduated biopsy forceps (DGBF).

Background Epigallocatechin gallate (EGCG) is a polyhydroxy phenolic compound extracted from tea and its antitumor effect has received widespread attention. We explored the inhibitory of EGCG on dimethylhydrazine (DMH)-induced colorectal cancer (CRC) using rat model, predicted interaction between CRC target genes database, explained associated pathways mechanisms in CRC. Aim To understand cell proliferation identify pharmacological targets by network pharmacology analysis. Methods DMH (40...

Objective: To explore whether SLBZD can play a synergistic role in promoting the efficacy of PD-1 inhibitors treatment colorectal cancer by influencing intestinal microenvironment and Tumor microenvironment.Method: Shenling Baizhu Decoction (SLBZD) tirelizumab (TLzmab) treated mouse model.The tumor growth rate, weight, inhibition rate were evaluated.Fecal microbiota was detected 16S rDNA sequencing immune cell flow cytometry analysis.Result: Compared to there exist significant differences...

To investigate whether Portulaca oleracea extract affects tumor formation in colon cancer stem cells and its chemotherapy sensitivity. In addition, to analyze associated genetic changes within the Notch signal transduction pathway. Serum-free cultures of (HT-29) HT-29 were treated with chemotherapeutic drug 5-fluorouracil assess Injections also given BALB/c mice confirm growth note characteristics. effect different concentrations P. was tested on cells, as determined by...

Background/Aim: Overexpression of inflammatory cytokines and oxidative stress increase the risk colorectal cancer (CRC) in obesity hyperlipidemia. The aim this study was to investigate whether monoterpene antioxidant p-cymene would reduce incidence CRC a rat model Materials Methods: hyperlipidemic established by high-fat diet dimethyl hydrazine (DMH) induction. All rats received 30 mg/kg DMH induce CRC, were then assigned groups with normal or with/without mg/kg/day orally during entire...

This study aimed to explore the curative effect of curcumin on liver fibrosis and its correlation with gut-liver axis in animal models. Histological staining was utilized conduct histological analysis intestine. An automatic biochemical analyzer or enzyme-linked immunosorbent assay system for analyzing indexes mice. Western blotting employed examine level relevant proteins. Furthermore, 16S rRNA high-throughput sequencing performed impact intestinal microorganisms rats fibrosis....

The objective of this study was to explore the expression and clinical significance Notch signaling genes in colorectal cancer. Colorectal cancer samples were prospectively collected from patients post-surgery at 3rd Affiliated Hospital, Nanjing University Traditional Chinese Medicine. Immunohistochemistry tissue arrays used analyze involved pathway. Microsatellite instability (MSI) detected by fluorescence multiplex polymerase chain reaction. A total 146 collected, including 84 men (57.7%)...

Objective: To investigate the preventing effect of P-cymene on high fat diet-related colorectal cancer and its mechanism.Methods: Forty Wistar rats were randomly divided into G1 group (high-fat diet), G2 diet + DMH), G3 P-cymene), G4 DMH P-cymene).G2 groups subcutaneously injected with dimethylhydrazine (DMH), intragastrically administered to effects tumor formation, inflammatory factors, glucose, lipid metabolism gut microbes.Results: No tumors formed in high-fat (G1) or P-cymone (G3).7...

China has the largest numbers of hereditary non‐polyposis colorectal cancer (HNPCC) patients based on its population 1.4 billion. However, clinical data and mismatch repair (MMR) gene analyses have been limited. Here we performed microsatellite instability (MSI) immunohistochemistry (IHC) a series with high‐risk for HNPCC: 61 family histories fulfilling Amsterdam criteria II (ACII‐HNPCC) or suspected HNPCC (S‐HNPCC), 106 early onset (CRC) patients. Sixty late‐onset CRC were used as control....

The orthotopic transplantation model of human tumor has been demonstrated to be more patient-like animal model. However, observations progression and metastasis are limited by the deep location colon or penetration ability fluorescence through tissue. purpose this study is establish a superficial allow easier real-time visualization sensitive monitoring fluorescent tumor. Human cancer HT-29 cells were transduced with pLPCX expression retroviral vector containing green protein neomycin...

This study aims to find the effect of onion's extraction on colorectal cancer with hyperlipidemia.We established a hyperlipidemia-subcutaneously heterotopic orthotopic transplant model and fed mice high fat diet performing transplantation. Animal models were treated capecitabine and/or simvastatin low-, middle-, high- dose both tumor growth rate blood lipid levels monitored.We found that in groups was significantly inhibited, equivalent capecitabine. Onion's effectively decreased apoB TC.Our...