A5023610618- Amyotrophic Lateral Sclerosis Research
- Estrogen and related hormone effects
- Microplastics and Plastic Pollution
- Parkinson's Disease Mechanisms and Treatments
- Neurogenetic and Muscular Disorders Research
- biodegradable polymer synthesis and properties
- Effects and risks of endocrine disrupting chemicals
- Plant Reproductive Biology
- Autophagy in Disease and Therapy
- Cytokine Signaling Pathways and Interactions
- Nanoparticles: synthesis and applications
- Adipose Tissue and Metabolism
- Biochemical Acid Research Studies
- Neurological diseases and metabolism
- Hypothalamic control of reproductive hormones
- Endoplasmic Reticulum Stress and Disease
- Protein Kinase Regulation and GTPase Signaling
- NF-κB Signaling Pathways
- Liver physiology and pathology
- Prion Diseases and Protein Misfolding
- Biochemical Analysis and Sensing Techniques
- Neuroscience and Neuropharmacology Research
- Pesticide and Herbicide Environmental Studies
- Angiogenesis and VEGF in Cancer
- Pesticide Exposure and Toxicity
University of Salerno
2021-2024
Medica (Italy)
2022
Dulbecco Telethon Institute
2014
Mario Negri Institute for Pharmacological Research
2008-2013
Istituti di Ricovero e Cura a Carattere Scientifico
2013
Roma Tre University
2005-2006
Sapienza University of Rome
1989-2000
University of Rome Tor Vergata
1989
Several neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), are characterized by the presence of misfolded proteins, thought to trigger neurotoxicity. Some familial forms ALS (fALS), clinically indistinguishable from sporadic (sALS), linked superoxide dismutase 1 (SOD1) gene mutations. It has been shown that mutant SOD1 misfolds, insoluble aggregates and impairs proteasome. Using transgenic G93A-SOD1 mice, we found spinal cord motor neurons, accumulating also...
In familial and sporadic amyotrophic lateral sclerosis (ALS) in rodent models of the disease, alterations ubiquitin-proteasome system (UPS) may be responsible for accumulation potentially harmful ubiquitinated proteins, leading to motor neuron death. spinal cord transgenic mice expressing ALS superoxide dismutase 1 (SOD1) gene mutation G93A (SOD1G93A), we found a decrease constitutive proteasome subunits during disease progression, as assessed by real-time PCR immunohistochemistry. parallel,...
Selenoprotein N (SEPN1) is a broadly expressed resident protein of the endoplasmic reticulum (ER) whose loss-of-function inexplicably leads to human muscle disease. We found that SEPN1 levels parallel those endoplamic oxidoreductin 1 (ERO1), an ER thiol oxidase, and SEPN1's redox activity defends from ERO1-generated peroxides. Moreover, we have defined redox-regulated interactome identified calcium import SERCA2 pump as redox-partner SEPN1. enhances by reducing luminal cysteines are...
Amyotrophic lateral sclerosis is heterogeneous with high variability in the speed of progression even cases a defined genetic cause such as superoxide dismutase 1 (SOD1) mutations. We reported that SOD1G93A mice on distinct backgrounds (C57 and 129Sv) show consistent phenotypic differences disease life-span are not explained by human SOD1 transgene copy number or burden mutant protein within nervous system. aimed to compare gene expression profiles motor neurons from these two mouse strains...
Amyotrophic lateral sclerosis (ALS) is a progressive fatal neurodegenerative disease characterised by loss of motor neurons that currently has no cure. Omega-3 polyunsaturated fatty acids, such as eicosapentaenoic acid (EPA), have many health benefits including neuroprotective and myoprotective potential. We tested the hypothesis high level dietary EPA could exert beneficial effects in ALS. The exposure to (300 mg/kg/day) well-established mouse model ALS expressing G93A superoxide dismutase...
The hypothalamus-pituitary-testis axis controls the production of spermatozoa, and kisspeptin system, comprising Kiss1 receptor (Kiss1R), is main central gatekeeper. activity system also occurs in testis but currently need peripheral to produce gametes not fully understood. Hence, we characterized rat spermatozoa epididymis caput cauda analyzed possible presence epididymal fluid. Kiss1R collected from was evaluated by Western blot; significant high levels (p < 0.001 vs. cauda) constant...
This study represents a pioneering investigation into using microfluidic technology for manufacturing PLA and PLGA nanocarriers (NCs) loaded with tracer molecules or metals through co-precipitation protocol that involves saturating the water phase. The effects of total flow rate (TFR), ratio (FRR), surfactant amount, polymer concentration on particle sizes distributions were examined. average size PLA-NCs varied from 349 ± 175 nm to 170 64 nm, surface charges ranging -13 -6 mV. In contrast,...
Bisphenol A (BPA) is largely used as a monomer in some types of plastics. It accumulates tissues and fluids able to bypass the placental barrier, affecting various organs systems. Due huge developmental processes, children, foetuses, neonates could be more sensitive BPA-induced toxicity. To investigate multi-systemic effects chronic exposure low BPA dose (100 μg/L), pregnant Wistar rats were exposed drinking water during gestation lactation. At weaning, newborn received same treatments dams...
Oxidative stress and mitochondrial impairment are the main pathogenic mechanisms of Amyotrophic Lateral Sclerosis (ALS), a severe neurodegenerative disease still lacking effective therapy. Recently, coenzyme-Q (CoQ) complex, key component function redox-state modulator, has raised interest for ALS treatment. However, while oxidized form ubiquinone10 was ineffective in patients modestly mouse models ALS, no evidence reported on effect reduced ubiquinol10, which better bioavailability...
Male reproduction is under the control of hypothalamus-pituitary-gonadal (HPG) axis. The endocannabinoid system (ECS) and kisspeptin (KS) are two major signaling systems in central peripheral reproduction, but their possible interaction has been poorly investigated mammals. This manuscript analyzes reciprocal modulation HPG axis.Adolescent male rats were treated with kisspeptin-10 (Kp10) anandamide (AEA), latter alone or combination type 1 cannabinoid receptor (CB1) antagonist rimonabant...
Abstract The relationship between cell proliferation and inositol lipid turnover has been studied by comparing the steady state of derivative metabolism in quiescent regenerating rat hepatocytes isolated at 4 h (G1 phase first cycle) 24 (onset M phase) after partial hepatectomy. effect two hormones able to regulate hepatic regeneration, insulin vasopressin, considered, results can be summarized as follows: (i) hepatectomy, precursor incorporation into polyphosphates particulate phospholipase...
System A‐mediated amino acid transport, activation of different steps signal transduction and involvement isoforms protein kinase C (PKC) have been investigated in chick embryo hepatocytes after epidermal growth factor (EGF) stimulation. EGF rapidly (10 min) increased the rate aminoisobutyric (AIB) uptake freshly isolated on 19th day embryonic life, while no change was detectable at other embryonal stages. The stimulation abolished by PKC tyrosine inhibitors mimicked...
Bisphenol A (BPA), commonly used as building block of polycarbonate plastics, significantly affects human and animal health interfering with the action natural hormones. Within BPA disrupting effects, a mitogenic activity and, consequently, an increased incidence neoplastic transformations has been reported in exposed organisms. Among several mechanisms proposed for its ability to bind estrogen receptors (ERα ERβ) deserves particular attention. Aim this work is investigate ERα- ERβ-dependent...
The knowledge of the molecular mechanism by which estrogens exert pleiotropic functions in different tissues and organs has evolved rapidly during past two decades. It is now well established that 17β-estradiol (E2) induces transcriptional regulation target gene expression upon binding to intracellular estrogen receptor-α (ERα) or -β (ERb). In addition E2 modulates cell through rapid non-genomic actions. Stimulation G-proteins, Ca2+ influx as phospholipase C, ERK/MAPK, PI3K/AKT activation...
Few studies have focused on the long-term effects of COVID-19 pandemic mental health. The objective our work was to evaluate changes in emotional and behavioral symptoms patients with neuropsychiatric disorders impact parenting stress 1 year after first national lockdown.We enrolled 369 aged 1.5-18 years age referred Child Adolescent Neuropsychiatry Unit University Hospital Salerno (Italy) by their parents. We asked parents complete two standardized questionnaires for assessment...
ERα is a ligand-activated transcription factor, member of the nuclear receptor superfamily. Regulation levels intrinsically required for its transcriptional activity and thus modulation physiological actions cognate hormone 17β-estradiol (E2). Indeed, exogenous ligands that target this molecular circuitry are used as drugs in clinical practice. Interestingly, some natural synthetic molecules, which human beings commonly exposed to, interfere with endocrine system operate through by...