A5024200861- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Neurobiology and Insect Physiology Research
- Legume Nitrogen Fixing Symbiosis
- Plant tissue culture and regeneration
- Insect and Arachnid Ecology and Behavior
- Plant Genetic and Mutation Studies
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Aluminum toxicity and tolerance in plants and animals
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Insect and Pesticide Research
- Genetics, Bioinformatics, and Biomedical Research
- Plant biochemistry and biosynthesis
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- Microbial Metabolites in Food Biotechnology
- GABA and Rice Research
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Plant and animal studies
Tobacco Research Institute
2011-2025
Chinese Academy of Agricultural Sciences
2011-2025
China Tobacco
2019-2025
Ministry of Agriculture and Rural Affairs
2021
Genetic linkage map is helpful for analysis on heredity of some gene families and map-based cloning because its simple elegant manifestation. One software in need to draw a physical map, which shows manner similar the genetic based relative distance between genes. Although tools like GBrowse MapViewer etc. are available there obvious limitations them: (1) data be decorated advance; (2) users can't modify results. Therefore, we developed bio-assisted mapping software--MapGene2Chrom with PC...
The NAC family is one of the largest families plant-specific transcription factors (TFs) and proteins play important regulatory roles in a variety developmental stress response processes plants. Members TFs have been shown to be regulators leaf senescence number plant species. Here we report identification tobacco (Nicotiana tabacum) characterization potential role some regulating senescence. A total 154 genes (NtNACs) were identified clustered together with Arabidopsis into fifteen groups...
Nuclear Factor Y (NF-Y) represents a group of transcription factors commonly present in higher eukaryotes, typically consisting three subunits: NF-YA, NF-YB, and NF-YC. They play crucial roles the embryonic development, photosynthesis, flowering, abiotic stress responses, other essential processes plants. To better understand genome-wide NF-Y domain-containing proteins, protein physicochemical properties, chromosomal localization, synteny, phylogenetic relationships, genomic structure,...
C2H2 zinc finger protein transcription factor family members have important biological functions in eukaryotes. They not only bind DNA and RNA, but also interact with proteins. In this study, 118 of the tobacco were identified from N. tabacum genome database by using Pfam, SMART Blastp. The analyses phylogenetic tree, physical chemical properties, chromosomal mapping, gene structures, three-dimensional structures tissue expression patterns performed. results suggested that peptide length...
Members of the plant-specific B3 transcription factor superfamily play important roles in various growth and developmental processes plants. Even though there are many valuable studies on genes other species, little is known about tobacco. We identified 114 proteins from tobacco using comparative genome analysis. These were classified into four subfamilies based their phylogenetic relationships, include ARF, RAV, LAV, REM subfamilies. The chromosomal locations, gene structures, conserved...
The basic leucine zipper (bZIP) transcription factors play important regulatory roles, influencing plant growth and responses to environmental stresses. In the present study, 132 bZIP genes identified in tobacco genome were classified into 11 groups with Arabidopsis tomato members, based on results of a phylogenetic analysis. An examination gene structures conserved motifs revealed relatively exon/intron motif organization within each group. an investigation whole-genome duplication events...
The coding products of WRKY gene family plays important roles in plant growth and development as well various stress responses. They have been identified plants, but only few common tobacco (Nicotiana tabacum L.). In this study, 164 putative proteins the genome were by using conserved sequence (PF03106) from Pfam database. Phylogenetic trees, functional domain analysis, chromosomal localization, subcellular localization tissue expression patterns analyzed with bioinformatics softwares,...
Abstract Background Color vision and phototactic behavior based on opsins are important for the fitness of insects because their roles in foraging mate choice. Related topics, including duplication loss opsin genes, have been well investigated insect orders such as Coleoptera, Lepidoptera, Hymenoptera, Odonata Orthoptera, findings used to develop pest management strategies involving light trapping. Mirid bugs Hemiptera, which pests that cause heavy economic losses, show capacity color...
Horizontal gene transfer and duplication are two major mechanisms contributing to the evolutionary adaptation of organisms. Previously, polygalacturonase genes (PGs) were independently horizontally transferred underwent multiple duplications in insects (e.g., mirid bugs beetles). Here, we chose three phytozoophagous (Adelphocoris suturalis, A. fasciaticollis, lineolatus) one zoophytophagous bug (Nesidiocoris tenuis) detect whether duplication, molecular evolution, expression levels PGs...
As the last stage of plant development, leaf senescence has a great impact on plant's life cycle. Genetic manipulation been used as an efficient approach in improving yield and quality crop plants. Here we describe ethyl methane sulfonate (EMS) mutagenesis induced premature mutant yellow 1 (yl1) common tobacco (Nicotiana tabacum L.). The yl1 plants displayed early yellowing. Physiological parameters marker genes expression indicated that phenotype was caused by senescence. analyses...
Cadmium (Cd) is known as one of the most hazardous elements in environment and a persistent soil constraint toxic to all flora fauna. In this study, we conducted physiological, biochemical, transcriptomic analyses Nicotiana rustica (N. rustica) tabacum tabacum) treated with CdCl2 know underlying molecular mechanisms Cd accumulation. As result, N. had more dry weight than tabacum. Additionally, accumulated higher concentration (69.65 times), Cd2+ influx (1.32-fold), glutathione S-transferases...
100081 摘要: microRNAs(miRNAs)是一类在真核生物中发现的长度为 21 nt 左右、非编码、内源性的单链小分子 RNA, 通过与靶基因的互补发挥转录后水平的负调控作用。目前, 已在许多物种中报道了 miRNAs 的存在, 然而还未 见关于茄子 的报道。根据 在植物中的高度保守性及其前体的二级结构特征, 文章通过同源预 测的方法, 将已知植物的 与茄子 EST 数据库比对, 经过一系列的筛选, 最终预测到 12 个家族的 16 条 茄子 miRNAs, 其中包括 3 个 miRNA 家族的正义/反义 而 miR390 和 miR399 属于第一次发现。文章还通过在线软件 psRNATarget 预测到 15 条茄子 的 71 个靶基因, 这些靶基因主 要编码与茄子生长发育、新陈代谢以及胁迫响应等过程相关的蛋白。
Abstract CRISPR/Cas9-mediated gene editing provides a powerful tool for dissecting function and improving important traits in crops. However, there are still persisting challenges to obtain high homozygous/bi-allelic (ho/bi) mutations dicot plants. Here, we develop an improved CRISPR/Cas9 system harboring calreticulin-like promoter, which can boost targeted dicots. Additionally, the pDC45_dsg construct, combining 35Spro-tRNA_sgRNA-EU unit PCE8pro -controlled Cas9, achieve more than 80.0%...