A5089404937- Hematopoietic Stem Cell Transplantation
- Photodynamic Therapy Research Studies
- Neutropenia and Cancer Infections
- Immunotherapy and Immune Responses
- Blood donation and transfusion practices
- Blood transfusion and management
- Erythrocyte Function and Pathophysiology
- Corneal Surgery and Treatments
- Platelet Disorders and Treatments
- COVID-19 Clinical Research Studies
- Sepsis Diagnosis and Treatment
- Hemoglobinopathies and Related Disorders
- Blood disorders and treatments
- SARS-CoV-2 and COVID-19 Research
- Blood groups and transfusion
- Antimicrobial Peptides and Activities
- Glaucoma and retinal disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ocular Infections and Treatments
- Chronic Lymphocytic Leukemia Research
- PI3K/AKT/mTOR signaling in cancer
- Cell death mechanisms and regulation
- Ocular Oncology and Treatments
- Renal Transplantation Outcomes and Treatments
- Blood properties and coagulation
University Hospital Regensburg
2016-2025
Institute for Transfusion Medicine
2018
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic challenges national health systems and the global economy. Monitoring of infection rates seroprevalence can guide public measures to combat pandemic. This depends on reliable tests active former infections. Here, we set out develop validate a specific sensitive enzyme linked immunosorbent assay (ELISA) for detection anti-SARS-CoV-2 antibody levels.In our ELISA, used SARS-CoV-2 receptor-binding domain (RBD) stabilized...
BACKGROUND Extracorporeal photopheresis (ECP) is a therapeutic technique that combines leukapheresis and ultraviolet (UV)A irradiation of the leukapheresate after 8‐methoxypsoralen treatment with subsequent retransfusion. It can be achieved single device (online) or by combining an apheresis machine separate UVA light source (offline). The comparability both established methods unknown. STUDY DESIGN AND METHODS In prospective setting, four ECP systems were evaluated: one integrated for...
BACKGROUND Patients with neutropenia or granulocyte dysfunction may require transfusions for adequate immune restoration. High‐molecular‐weight hydroxyethyl starch (HES) is the most commonly used sedimentation agent to enhance collection efficiency. However, authorities recently restricted use of HES due its unfavorable risk–benefit profile. As modified fluid gelatin (MFG) already as an alternative agent, we tested hypothesis that MFG not inferior in terms functionality and viability...
BACKGROUND Granulocyte apheresis requires a sedimentation agent. Usually, hydroxyethyl starch (HES) is administered to donors for this purpose and, as granulocyte concentrate (GC) ingredient, also patients. Authorities recently recommended suspending market authorizations starch‐containing products due side effects. Therefore, we tested the efficacy of modified fluid gelatin (MFG, Gelafundin 4%) versus hetastarch (Hespan) GC apheresis. STUDY DESIGN AND METHODS This retrospective matched‐pair...
Abstract Background and aims Serum eye drops alleviate ocular symptoms of diseases such as sicca syndrome, or chronic graft‐versus‐host disease. This study was designed for good manufacturing practice validation our standard manufacturing, storage transport processes both autologous allogenic SEDs. Specifications quality parameters are lacking were aimed to be defined. Methods Using sterile collected, coagulated whole blood, serum separated by centrifugation filled into single‐use drop...
The ongoing coronavirus disease 2019 (COVID-19) pandemic emerged in December 2019. Convalescent plasma represents a promising COVID-19 treatment. Here, we report on the manufacturing of plasma-based product containing antibodies specific to SARS-CoV-2 obtained from recently recovered patients. donors were screened as follows: 1) previously confirmed infection (by real-time PCR (RT-PCR)); 2) subsequent negative test followed by 2-week waiting period; 3) an additional prior plasmapheresis; and...
Background Several sensitive methods are available for red blood cell (RBC) antibody screening. Among these, gel and glass card systems have demonstrated comparably good performance in retrospective studies widely used routine patient diagnostics, but their prospective has not been sufficiently characterised. Patients Gel (Bio-Rad DiaMed) bead-based (Ortho Clinical Diagnostics) column agglutination technologies were to screen antibodies prospectively (group A) identification stored fresh...
Extracorporeal photopheresis (ECP) is an efficient method to treat various autoimmune diseases, cutaneous T-cell lymphoma, and graft-versus-host disease. It based on the ex vivo inactivation of lymphocytes by 8-methoxypsoralen (8-MOP)/UV light treatment. Despite adhesive, lipophilic nature 8-MOP, no quality control established for ECP procedure.We developed a sensitive high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) assay monitor residual 8-MOP concentration...
Plateletpheresis using a leukocyte reduction system (LRS) traps donor WBCs in the LRS chamber, which may lead to lymphopenia, especially frequent plateletpheresis donors. It seems plausible that this might cause adverse effects. However, current knowledge about potential confounders and health impacts is incomplete.Recent platelet donors donations collected at University Hospital Regensburg from 2016 2019 Terumo BCT Trima Accel were retrospectively analyzed compared with historical mainly...
Abstract Background Extracorporeal photopheresis (ECP), an apheresis‐based therapy for various immunological diseases, works mainly by inducing apoptosis in lymphocytes. Several factors influence the efficacy of ECP with photosensitizer 8‐methoxypsoralen (8‐MOP) and ultraviolet light A (UVA). This study aimed to optimize treatment varying 8‐MOP starting concentration cell suspension medium. Materials Methods All patients ( n = 13) included this received as medically indicated. Cells...
Allogeneic hematopoietic stem cell transplantation (HSCT) is the only curative treatment for SCD and bone marrow from an HLA-matched sibling currently standard of care. Haploidentical HSCT a family donor with TCR αβ/CD19 depleted graft (T-haplo) increasingly successful alternative, which requires generation G-CSF stimulated peripheral (PBSC) haploidentical relatives. These sickle trait (SCT) donors reported to develop SCD-related complications in conditions severe stress.In this...
ABSTRACT BACKGROUND Extracorporeal photopheresis (ECP) is an immunosuppressive treatment that involves leukocyte apheresis, psoralen and UV light treatment, subsequent reinfusion. Patients treated with ECP are usually immunosuppressed. Bacterial contamination therefore poses a much unwanted risk, but incidence data lacking. PATIENTS AND METHODS We screened all 1922 consecutive procedures scheduled within roughly 3‐year period for eligibility. Those missing on method (inline or offline) type...
Background: Extracorporeal photopheresis (ECP) is an effective immunomodulatory therapy for various diseases. Autologous leukocytes are collected, photoactivated with a photosensitizer (8–methoxypsoralen, 8–MOP) and UVA light, subsequently reinfused back to the patient. Leukapheresis rradiation systems can be integrated into one device (inline) or handled by two separate devices (offline) ECP works via intercalation of 8–MOP DNA helices UVA-based interactions inhibit replication. known...
Platelets are prone to activation from handling; they therefore transported as gently possible, most commonly by courier. Speedier methods like pneumatic tube system (PTS) transport could improve patient care but may subject platelets mechanical stress. To test the impact of stress caused transport, we compared a PTS with conveyor box and courier on apheresis platelet function.Fourteen concentrate triple donations were analyzed light transmission aggregometry (LTA), rotational...
Abstract Background More granulocyte concentrates (GCs) could be produced for more patients from the same donor if apheresis bags were split and stored longer periods of time. Hence, we tested hypothesis that splitting extension storage GCs do not impair function or viability. Study Design Methods Granulocyte using modified fluid gelatin as a separation enhancer, into two portions, 24 48 h. function, represented by cell migration, reactive oxygen species (ROS) production, neutrophil...
Abstract Objectives This validation study investigated a flow cytometric apoptosis assay according to good manufacturing practice (GMP). Background Extracorporeal photopheresis (ECP) is treatment for various immunological diseases and cutaneous T‐cell lymphomas. It based on the induction of by 8‐methoxypsoralene ultraviolet A light. The quantification therefore essential ECP improvements. However, despite numerous publications apoptosis, validated technical details are lacking. Methods...
Zusammenfassung Die Extrakorporale Photopherese ist ein etabliertes Therapieverfahren für Patienten mit T-Zell vermittelten Erkrankungen. Dabei besteht das Verfahren aus der Gewinnung autologer mononukleärer Zellen, deren Behandlung 8-Methoxypsoralen und UVA-Licht die Retransfusion behandelten Zellen. Wirkmechanismen sind zwar noch nicht vollständig geklärt, zentraler Mechanismus stellt jedoch Apoptose Zellen dar. Das Ziel Studie war eine Optimierung im Hinblick auf 8-Methoxypsoralen/UVA...