A5030200967- Hemoglobinopathies and Related Disorders
- COVID-19 Clinical Research Studies
- Blood donation and transfusion practices
- Blood transfusion and management
- SARS-CoV-2 and COVID-19 Research
- Heat shock proteins research
- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Medical History and Innovations
- Neutropenia and Cancer Infections
- Medical History and Research
- Hematopoietic Stem Cell Transplantation
- Blood disorders and treatments
- Immune Cell Function and Interaction
- Central European and Russian historical studies
- PARP inhibition in cancer therapy
- Virology and Viral Diseases
- Toxin Mechanisms and Immunotoxins
- Radiation Therapy and Dosimetry
- Long-Term Effects of COVID-19
- Platelet Disorders and Treatments
- History of Medicine Studies
- Reformation and Early Modern Christianity
- Linguistic and Cultural Studies
- PI3K/AKT/mTOR signaling in cancer
University of Regensburg
2025
University Hospital Regensburg
2015-2024
Institute for Transfusion Medicine
2002
Bristol Institute for Transfusion Sciences
1996
DRK-Blutspendedienst Baden-Württemberg - Hessen
1994
Bayer (Germany)
1994
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic challenges national health systems and the global economy. Monitoring of infection rates seroprevalence can guide public measures to combat pandemic. This depends on reliable tests active former infections. Here, we set out develop validate a specific sensitive enzyme linked immunosorbent assay (ELISA) for detection anti-SARS-CoV-2 antibody levels.In our ELISA, used SARS-CoV-2 receptor-binding domain (RBD) stabilized...
The role of venovenous extracorporeal membrane oxygenation (VV ECMO) in patients with COVID-19-induced acute respiratory distress syndrome (ARDS) still remains unclear. Our aim was to investigate the clinical course and outcome those identify factors associated need for prolonged ECMO therapy.A retrospective single-center study on VV COVID-19-associated ARDS performed. Baseline characteristics, ventilatory parameters, laboratory virological results were evaluated over time. Six months...
More than 40 human cases of severe encephalitis caused by Borna disease virus 1 (BoDV-1) have been reported to German health authorities. In an endemic region in southern Germany, we conducted the seroepidemiological BoSOT study ("BoDV-1 after solid-organ transplantation") assess whether there are undetected oligo- or asymptomatic courses infection. A total 216 healthy blood donors and 280 outpatients solid organ transplantation were screened a recombinant BoDV-1 ELISA followed indirect...
Abstract Mesenchymal stem cells and multipotent adult progenitor (MAPCs) have been proposed as novel therapeutics for solid organ transplant recipients with the aim of reducing exposure to pharmacological immunosuppression its side effects. In present study, we describe clinical course first patient phase I, dose-escalation safety feasibility MiSOT-I (Mesenchymal Stem Cells in Solid Organ Transplantation Phase I). After receiving a living-related liver graft, was given one intraportal...
Abstract Purpose: Non–small cell lung cancer (NSCLC) is a fatal disease with poor prognosis. A membrane-bound form of Hsp70 (mHsp70) which selectively expressed on high-risk tumors serves as target for mHsp70-targeting natural killer (NK) cells. Patients advanced mHsp70-positive NSCLC may therefore benefit from therapeutic intervention involving NK The randomized phase II clinical trial (EudraCT2008-002130-30) explores tolerability and efficacy ex vivo–activated cells in patients after...
Abstract Life expectancy of patients with severe transfusion-dependent beta-thalassemia (TDT) remains below that the general population. Allogenic hematopoietic stem cell transplantation (HSCT) is standard curative treatment. Due to paucity matched donor (MD) availability, haploidentical HSCT (haplo-HSCT) a reasonable alternative. Twenty TDT (median age 10 years; range 2–23) received either sibling (MSD; n = 7) or haplo-HSCT ( 13) in single center (Regensburg, Germany) between 2016 and 2022,...
More than 40 human infections with the zoonotic Borna disease virus 1 (BoDV-1) have been reported to German health authorities from endemic regions in southern and eastern Germany. Diagnosis of a confirmed case is based on detection BoDV-1 RNA or antigen. In parallel, serological assays such as ELISA, immunoblots, indirect immunofluorescence are use detect seroconversion virus-reactive IgG serum cerebrospinal fluid (CSF). As immunopathogenesis encephalitis appears be driven by T cells, we...
BACKGROUND: McLeod neuroacanthocytosis syndrome is a late‐onset X‐linked multisystem disorder affecting the peripheral and central nervous systems, red blood cells (RBCs), internal organs. A variety of mutations have been found in responsible gene ( XK ) including single nonsense missense mutations, nucleotide at or near splice junctions introns , different deletion mutations. To date no clear phenotype‐genotype correlation apparent. The clinical details one case phenotype without apparent...
Abstract Background To date, in‐depth analysis of leukapheresis products as starting material for CAR T‐cell manufacturing, specifically Tisagenlecleucel production, are scarce. In this study, we report on lymphapheresis data production elderly and pretreated lymphoma patients. Study Design Methods Spectra Optia from Terumo BCT, Lakewood, CO, was employed apheresis using the cMNC program. Apheresis success defined meeting a target total nucleated cell (TNC) count ≥2 × 10 9 , CD3‐positive...
BACKGROUND Granulocyte apheresis requires a sedimentation agent. Usually, hydroxyethyl starch (HES) is administered to donors for this purpose and, as granulocyte concentrate (GC) ingredient, also patients. Authorities recently recommended suspending market authorizations starch‐containing products due side effects. Therefore, we tested the efficacy of modified fluid gelatin (MFG, Gelafundin 4%) versus hetastarch (Hespan) GC apheresis. STUDY DESIGN AND METHODS This retrospective matched‐pair...
Abstract Background and aims Serum eye drops alleviate ocular symptoms of diseases such as sicca syndrome, or chronic graft‐versus‐host disease. This study was designed for good manufacturing practice validation our standard manufacturing, storage transport processes both autologous allogenic SEDs. Specifications quality parameters are lacking were aimed to be defined. Methods Using sterile collected, coagulated whole blood, serum separated by centrifugation filled into single‐use drop...
The ongoing coronavirus disease 2019 (COVID-19) pandemic emerged in December 2019. Convalescent plasma represents a promising COVID-19 treatment. Here, we report on the manufacturing of plasma-based product containing antibodies specific to SARS-CoV-2 obtained from recently recovered patients. donors were screened as follows: 1) previously confirmed infection (by real-time PCR (RT-PCR)); 2) subsequent negative test followed by 2-week waiting period; 3) an additional prior plasmapheresis; and...
Plateletpheresis using a leukocyte reduction system (LRS) traps donor WBCs in the LRS chamber, which may lead to lymphopenia, especially frequent plateletpheresis donors. It seems plausible that this might cause adverse effects. However, current knowledge about potential confounders and health impacts is incomplete.Recent platelet donors donations collected at University Hospital Regensburg from 2016 2019 Terumo BCT Trima Accel were retrospectively analyzed compared with historical mainly...
Abstract Background Extracorporeal photopheresis (ECP), an apheresis‐based therapy for various immunological diseases, works mainly by inducing apoptosis in lymphocytes. Several factors influence the efficacy of ECP with photosensitizer 8‐methoxypsoralen (8‐MOP) and ultraviolet light A (UVA). This study aimed to optimize treatment varying 8‐MOP starting concentration cell suspension medium. Materials Methods All patients ( n = 13) included this received as medically indicated. Cells...
Ein ungewöhnlicher Brief berichtet über die Ursache der mehrjährigen Verzögerung Edition eines Traktates zum „Aufstieg und Niedergang“ Blutübertragung im 17. Jahrhundert.[1] Nach den ersten gut dokumentierten vielversprechenden Bluttransfusionen von Hund zu Lamm Mensch (Richard Lower Edmund King, London 1665, Jean-Baptiste Denis Paul Emmerez, Paris 1667, Matthäus Gottfried Purmann, 1668) wuchs zunächst das Interesse an diesem experimentellen Eingriff, getrieben Neugierde sowie theologischer...
Zusammenfassung Die therapeutische Verwendung von Hydroxyethylstärke (HES) unterliegt wegen der Risiken besonderen Auflagen. Inzwischen wird auch dessen Anwendung als Sedimentationsbeschleuniger in Gewinnung und Herstellung Granulozytenkonzentraten (GK) abgeraten. Im vorliegenden Beitrag vorgestellt, wie statt HES (HESpan 6%) modifizierte flüssige Gelatine (4% Gelafundin) Alternative eingesetzt werden kann. Hierzu Klinikapotheke eine Mischung Gelafundin 4% Seracit 1M steril angefertigt....
Allogeneic hematopoietic stem cell transplantation (HSCT) is the only curative treatment for SCD and bone marrow from an HLA-matched sibling currently standard of care. Haploidentical HSCT a family donor with TCR αβ/CD19 depleted graft (T-haplo) increasingly successful alternative, which requires generation G-CSF stimulated peripheral (PBSC) haploidentical relatives. These sickle trait (SCT) donors reported to develop SCD-related complications in conditions severe stress.In this...
Abstract Background Granulocyte transfusions (GT) are used to treat progressive systemic or local infections in prolonged neutropenic patients with antibiotic antifungal resistance. Granulocytes most commonly collected from whole blood by apheresis using hydroxyethyl starch (HES) as the red cell (RBC) sedimentation agent. This is first study on safety and efficacy of transfusing granulocytes modified fluid gelatin (MFG) instead HES pediatric patients. Methods Clinical data 46 adolescent...
Platelets are prone to activation from handling; they therefore transported as gently possible, most commonly by courier. Speedier methods like pneumatic tube system (PTS) transport could improve patient care but may subject platelets mechanical stress. To test the impact of stress caused transport, we compared a PTS with conveyor box and courier on apheresis platelet function.Fourteen concentrate triple donations were analyzed light transmission aggregometry (LTA), rotational...
The COVID-19 pandemic reached Bavaria in February 2020. Almost simultaneously, Chinese physicians published reports on the first successful treatments with plasma from convalescent donors. With these silver linings horizon, we decided to establish manufacturing of anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody-containing donors at our site. Here describe donor selection process, built ground up, which enabled us cope immense resonance after social media call for...