A5087939879- Protist diversity and phylogeny
- Marine and coastal ecosystems
- Microbial Community Ecology and Physiology
- Diatoms and Algae Research
- Parasite Biology and Host Interactions
- Monoclonal and Polyclonal Antibodies Research
- Marine Toxins and Detection Methods
- Legume Nitrogen Fixing Symbiosis
- Biotin and Related Studies
- Health and Medical Studies
- Coastal wetland ecosystem dynamics
- Click Chemistry and Applications
- Genetic diversity and population structure
- Telemedicine and Telehealth Implementation
- COVID-19 and Mental Health
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Animal Behavior and Reproduction
- Algal biology and biofuel production
- Plant nutrient uptake and metabolism
- Parasitic Infections and Diagnostics
- Helicobacter pylori-related gastroenterology studies
- bioluminescence and chemiluminescence research
- Genomics and Phylogenetic Studies
- Social Policies and Healthcare Reform
Max Planck Institute for Medical Research
2021-2024
Bielefeld University
2006-2023
University Hospital Regensburg
2023
Merck (Germany)
2020
Apogenix (Germany)
2016
Max Planck Institute for Ornithology
2012
Max Planck Society
2012
Medizinische Hochschule Hannover
2011
University of Bremen
1998-2008
Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung
1996-2005
High genetic diversity is a hallmark of the gastric pathogen Helicobacter pylori. We used 454 sequencing technology to perform whole-genome comparisons for five sets H. pylori strains that had been sequentially cultured from four chronically infected Colombians (isolation intervals=3-16 y) and one human volunteer experimentally with as part vaccine trial. The genomes Colombian differed by 27-232 isolated SNPs 16-441 imported clusters polymorphisms resulting recombination. Imports (mean...
The self-labeling protein tags (SLPs) HaloTag7, SNAP-tag, and CLIP-tag allow the covalent labeling of fusion proteins with synthetic molecules for applications in bioimaging biotechnology. To guide selection an SLP–substrate pair provide guidelines design substrates, we report a systematic comparative study kinetics substrate specificities CLIP-tag. HaloTag7 reaches almost diffusion-limited rate constants certain rhodamine which are more than 2 orders magnitude higher those SNAP-tag...
We introduce a family of bright, rhodamine-based calcium indicators with tuneable affinities and colors. The can be specifically localized to different cellular compartments are compatible both fluorescence bioluminescence readouts through conjugation HaloTag fusion proteins. Importantly, their increase in upon localization enables no-wash live-cell imaging, which greatly facilitates use biological assays. Applications as fluorescent rat hippocampal neurons include the detection single...
Populations forced through bottlenecks typically lose genetic variation and exhibit inbreeding depression. ‘Genetic rescue’ techniques that introduce individuals from outbred populations can be highly effective in reversing the deleterious effects of inbreeding, but have limited application for majority endangered species, which survive only a few bottlenecked populations. We tested effectiveness using inbred as donors to rescue two isolated South Island robin ( Petroica australis )....
More than 40 human cases of severe encephalitis caused by Borna disease virus 1 (BoDV-1) have been reported to German health authorities. In an endemic region in southern Germany, we conducted the seroepidemiological BoSOT study ("BoDV-1 after solid-organ transplantation") assess whether there are undetected oligo- or asymptomatic courses infection. A total 216 healthy blood donors and 280 outpatients solid organ transplantation were screened a recombinant BoDV-1 ELISA followed indirect...
Six microsatellite markers were used to investigate the genetic structure of North Sea Pseudo‐nitzschia pungens (Grunow ex P. T. Cleve) Hasle populations. Isolates collected on 42 separate occasions from waters surrounding German islands Helgoland and Sylt over course three sampling periods: spring 2002, 2003, autumn 2003. In total, 464 isolates genotyped, which 453 different (i.e. clonal diversity was 98%). The numbers alleles per locus ranged 6 24 observed heterozygosities 0.59 0.87 (mean...
Site-specific bioconjugation technologies are frequently employed to generate homogeneous antibody–drug conjugates (ADCs) and generally considered superior stochastic approaches like lysine coupling. However, most of the developed so far require undesired manipulation antibody sequence or its glycan structures. Herein, we report successful engineering microbial transglutaminase enabling efficient, site-specific conjugation drug-linker constructs position HC-Q295 native, fully glycosylated...
ABSTRACT The genome of Sinorhizobium meliloti type strain Rm1021 consists three replicons: the chromosome and two megaplasmids, pSymA pSymB. Additionally, many indigenous S. strains possess one or more smaller plasmids, which represent accessory this species. Here we describe complete nucleotide sequence an plasmid, designated pSmeSM11a, that was isolated from a dominant subpopulation in context long-term field release experiment with genetically modified strains. Sequence analysis plasmid...
Abstract SNAP-tag is a powerful tool for labeling proteins with synthetic fluorophores in bioimaging. However, its utility live-cell applications can be constrained by relatively slow kinetics and the limited cell permeability of substrates. Here we introduce new substrates an engineered faster vitro live cells. SNAP-tag2 presents second-order rate constant rhodamine that approaches 10 7 s -1 M , 100-fold improvement over corresponding SNAP-tag-substrate pairs. When labeled highly...
Plasmid pSmeSM11a, residing in the indigenous Sinorhizobium meliloti strain SM11 originating from a field Strassmoos (Bavaria, Germany), was analysed previously at genomic level. Thirty-seven S. strains, two different locations Germany, were screened for genes identified on pSmeSM11a. Seven of these strains harbour accessory plasmids that are very similar to The pSmeSM11a-like c. 130-150 kb size and possess nearly identical restriction profiles. Up 30 pSmeSM11a could be detected by...
The influence of turbulence on the incidence infection diatom Coscinodiscus granii by parasitoid nanoflagellate Pirsonia diadema was investigated experimentally with two initial host densities. Independently densities 7 and 44 cells ml–1, under calm conditions both diatoms parasitoids became extinct within 6–9 days. Turbulence, however, led to survival at a reduced density 0.1–2 ml–1 for >30 A population-dynamic model is formulated that takes into account non-homogeneous distribution...
The membrane dye FM1-43 was used to visualise internalisation in living cells of the marine centric diatoms Coscinodiscus wailesii and Thalassiosira punctigera. results revealed dynamic selective trafficking several cellular structures which have not been described light microscopical studies. Intimate regular contacts were found between plasma labiate processes inner face frustule C. wailesii. central cytoplasmic region close tonoplast growing parts silica deposition vesicles became...
The parasitoid nanoflagellate (PNF) Pirsonia diadema is host specific for the marine centric diatom Coscinodiscus spp. Experiments showed that flagellates significantly prefer C. wailesii over C.granii as species (interspecific selectivity). This preference was independent of light conditions (dark, irradiance 10 and 70 μmol m −2 s −1 ) temperature (10 15°C). Among unicellular diatoms, infection behaviour selective individual cells: already infected cells were more attractive further...
The pH on the frustule of individual cells marine centric diatoms Coscinodiscus granii and wailesii (Bacillariophyceae) was measured with microsensors in culture media increasing values 8.04, 8.14, 8.22, respectively. In 85–96% C. up to 0.4 units higher than that medium, reaching a maximum 8.95. Only 2–3% surface exceeded medium by 0.7 units. These results strongly suggest batch cultures differ, at least temporarily, their photosynthetic activities. Infection experiments parasitoid...