A5044914248- Glioma Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Neurogenesis and neuroplasticity mechanisms
- Neuroblastoma Research and Treatments
- Protease and Inhibitor Mechanisms
- Cancer, Hypoxia, and Metabolism
- Pluripotent Stem Cells Research
- Nerve injury and regeneration
- Peptidase Inhibition and Analysis
- RNA Interference and Gene Delivery
- Signaling Pathways in Disease
- Mitochondrial Function and Pathology
- Renal and related cancers
- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- S100 Proteins and Annexins
- ATP Synthase and ATPases Research
- Cancer Research and Treatments
- Force Microscopy Techniques and Applications
- Barrier Structure and Function Studies
- Nanoparticle-Based Drug Delivery
- RNA modifications and cancer
- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
University of Portsmouth
2014-2024
Brain Tumour Research
2015-2019
Cardiff University
2018
Centre National de la Recherche Scientifique
2015
CEA Paris-Saclay
2015
Institut de Biologie Intégrative de la Cellule
2015
Université Paris-Saclay
2015
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015
Université Paris-Sud
2015
King's College Hospital
2015
In this communication, we describe the successful encapsulation of 177Lu into endohedral metallofullerene 177LuxLu3−xN@C80 (x = 1−3) starting with 177LuCl3 in a modified quartz Kräschmer−Huffman electric generator. We demonstrate that (β-emitter) fullerene cage is not significantly released for period up to at least one-half-life (6.7 days). also agent can be conjugated an interleukin-13 peptide designed target overexpressed receptor glioblastoma multiforme tumors. This nanoparticle delivery...
Background: Glioblastoma multiforme is the most common and lethal primary brain tumor in humans, with median survival of approximately 1 year. Owing to ability glioma cells aggressively infiltrate normal tissue survive exposure current adjuvant therapies, there a great need for specific targeted nanoplatforms capable delivering both therapeutic imaging agents directly invasive cells. Method: Gadolinium-containing endohedral fullerenes, highly efficient contrast MRI, were functionalized...
The blood-brain barrier (BBB) consists of endothelial cells, astrocytes, and pericytes embedded in basal lamina (BL). Most vitro models use nonhuman, monolayer cultures for therapeutic-delivery studies, relying on transendothelial electrical resistance (TEER) measurements without other tight-junction (TJ) formation parameters. We aimed to develop reliable, reproducible, 3-dimensional (3D) incorporating relevant human, vivo cell types BL proteins. 3D BBB were constructed with human brain...
Abstract The interaction between extracellular matrix (ECM) and regulatory metalloproteinases (MMPs) is important in establishing maintaining synaptic connectivity. By using fluid percussion traumatic brain injury (TBI) combined TBI bilateral entorhinal cortical lesion (TBI + BEC), we previously demonstrated that hippocampal stromelysin‐1 (MMP‐3) expression activity increased during plasticity. We now report a temporal analysis of MMP‐3 protein mRNA response to both degenerative (2 day)...
Microarray technologies have come into prominence for the assessment of molecular diagnostic profiles in cancer tissue biopsies. To better understand effect sampling bias, we paired image-guided stereotactic biopsy and microarray technology to study regional intratumoral differences tumor periphery core regions untreated glioblastoma. RNA was extracted from serial frozen sections using an integral histopathologic scoring approach. Gene expression analysis performed high-density...
Medulloblastoma is the most common malignant paediatric brain tumour and current therapies often leave patients with severe neurological disabilities. Four major molecular groups of medulloblastoma have been identified (Wnt, Shh, Group 3 4), which include additional, recently defined subgroups different prognosis genetic characteristics. Lactate dehydrogenase A (LDHA) a key enzyme in aerobic glycolysis pathway, an abnormal metabolic pathway commonly observed cancers, associated progression...
Metastatic non-small cell lung (NSCLC) cancer represents one of the most common types brain metastasis. The mechanisms involved in how circulating cells transmigrate into parenchyma are not fully understood. aim this work was to investigate role fucosylated carbohydrate epitopes CD15 and sialyated CD15s adhesion brain-derived endothelial determine their influence blood-brain barrier (BBB) disruption METHODS: Three distinct, independent methods were used measure integrity include voltohmmeter...
Extracellular matrix (ECM) molecules, including chondroitin-4 or chondroitin-6 sulfate proteoglycans (CSPGs) and tenascin, are upregulated in around wounds transplants to the adult CNS. In present study, striatal from mice were used a novel vitro paradigm assess effects of these wound-associated molecules on embryonic dopamine cell attachment neurite outgrowth. Light electron microscopic immunocytochemistry studies have shown that astroglial scar constituents persist cultured explants for at...
Object. The goal of this study was to determine whether adenoviral vector—mediated expression human wildtype p53 can enhance the radiosensitivity malignant glioma cells that express native wild-type . gene is thought function abnormally in majority gliomas, although it has been demonstrated be mutated only approximately 30%. This led studies which transduction with investigated an attempt slow tumor cell growth. Recent suggest reconstitution render more susceptible radiation-mediated death,...
Object. High-flow microinfusion is a novel technique for delivery of compounds directly into brain parenchyma, bypassing the blood-brain barrier. The feasibility this has been demonstrated with low-molecular-weight compounds, macromolecular dyes, and proteins. Delivery antisense oligonucleotides parenchyma represents an additional potential application not previously described. In report authors sought to examine distribution disposition phosphorothioate oligodeoxynucleotide (PS-ODN) reason....
The Wilms tumor 1 (WT1) protein-a developmentally regulated transcription factor-is aberrantly expressed in gliomas and promotes their malignant phenotype. However, little is known about the molecular allies that help it mediate its oncogenic functions glioma cells.The authors used short interfering RNA (siRNA) to suppress WT1 expression glioblastoma (GBM) cells evaluated effect of this on GBM cell invasiveness. Gene analysis was then identify candidate genes were altered as a result...
Brain metastases are the most common fatal complication of systemic cancer, especially lung (40–50%) and breast (20–30%) cancers. In this era personalized therapy, there is a critical need to uncover signaling architecture brain metastases; however, little known about what pathways activated in context microenvironment. Using unique study set 42 from patients with or nonsmall cell cancer (NSCLC), phosphorylation/activation states 128 key proteins involved were measured laser capture...
CD15, which is overexpressed on various cancers, has been reported as a cell adhesion molecule that plays key role in non-CNS metastasis. However, the of CD15 brain metastasis largely unexplored. This study provides better understanding CD15/CD62E interaction, enhanced by tumor necrosis factor-α (TNF-α), and its correlation with non–small lung cancer (NSCLC). E-selectin (CD62E) expression was demonstrated both human primary metastatic NSCLC cells using flow cytometry, immunofluorescence,...
Glioblastoma (GBM) is the most common primary brain tumor in adults, with a dismal prognosis. Treatment hampered by GBM's unique biology, including differential cell response to therapy. Although several mitochondrial abnormalities have been identified, how DNA (mtDNA) mutations contribute GBM biology and therapeutic remains poorly described. We sought determine spectrum of functional complex III IV mtDNA GBM. The complete genomes 10 lines were obtained using next-generation sequencing...