A5101509265- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Circular RNAs in diseases
- Connective Tissue Growth Factor Research
- Cancer Research and Treatments
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- interferon and immune responses
- Electric Power System Optimization
- Renal cell carcinoma treatment
- Cell death mechanisms and regulation
- Liver Disease Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Inflammatory mediators and NSAID effects
- RNA Research and Splicing
- Viral Infections and Immunology Research
- Cancer and biochemical research
- Liver physiology and pathology
- Advanced X-ray and CT Imaging
- Biochemical and Molecular Research
- NF-κB Signaling Pathways
- Microgrid Control and Optimization
- Cancer Cells and Metastasis
- Peroxisome Proliferator-Activated Receptors
Shandong Provincial Hospital
2024
Shandong First Medical University
2024
Nanfang Hospital
2009-2024
Southern Medical University
2009-2024
Dalian University of Technology
2022-2024
Jinan Central Hospital
2024
Daping Hospital
2023
Army Medical University
2020-2023
Dalian University
2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2018-2020
Abstract The tripartite motif (TRIM) 22 and mitogen-activated protein kinase (MAPK) signaling pathways play critical roles in the growth of glioblastoma (GBM). However, molecular mechanism underlying relationship between TRIM22 MAPK remains unclear. Here, we found that binds to exon 2 sphingosine ( SPHK2 ) gene. An ERK1/2-driven luciferase reporter construct identified as a potential activator signaling. Knockout overexpression regulate inhibition activation through RING-finger domain....
With the widespread application of distributed power generation, traditional energy consumers are becoming active prosumers. Giving full play to role electricity market in resource allocation, peer-to-peer (P2P) trading between multiple prosumers and helps increase revenue sales reduce cost consumption. The participation flexible resources P2P achieves local complementarity, improves benefits, reduces operating pressure lines. To ensure benefits stimulate their enthusiasm, it is necessary...
AIM:To investigate the regulative effect of miRNA-338-3p (miR-338-3p) on cell growth in colorectal carcinoma (CRC). METHODS:The lentiviral vector pLV-THM-miR-338-3p and pLV-THM-miR-338-3p-inhibitor were constructed.The recombinant viral encoding pre-miR-338-3p or miR-338-3p-inhibitor two packaging plasmids psPAX2 pMD2.G cotransfected into human embryonic kidney 293T cells to package lentivirus.The supernatant containing lentivirus particles was harvested determine titer, this then used...
Background miR-338-3p is a recently discovered miRNA and involved in cell differentiation. However, few data are yet available on the aberrant expression of human colorectal carcinoma (CRC). This work aimed to investigate relationship between pattern clinicopathological features CRC possible regulative mechanisms. Methods The 40 CRC, adjacent nontumorous tissues 2 CRC-derived lines (SW-480 SW-620) were collected, respectively, total RNA protein isolated routinely. was detected by real-time...
Abstract Gastric cancer (GC) contains subpopulations of stem cells (CSCs), which are described as the main contributors in tumor initiation and metastasis. It is necessary to clarify molecular mechanism underlying CSCs phenotype develop novel biomarkers therapeutic targets for gastric cancer. Here, we show that POLQ positively regulates cell-like characteristics cells, knockdown suppressed stemness GC vitro vivo. Further mechanistic studies revealed could downregulate expression...
Abstract Acute coronary syndrome caused by the rupture of atherosclerotic plaques is one primary causes cerebrovascular and cardiovascular events. Neovascularization within plaque closely associated with its stability. Long non‐coding RNA (lncRNA) serves a crucial role in regulating vascular endothelial cells (VECs) proliferation angiogenesis. In this study, we identified lncRNA HCG11, which highly expressed patients vulnerable compared stable plaque. Then, functional experiments showed that...
We assessed the diagnostic value of "one-step" dual energy CT (DECT) in combination with coronary angiography and iodine mapping within myocardial blood pool detecting acute infarction (AMI).Five minipigs were subjected to transcatheter embolization artery a gelatin sponge induce AMI. Arterial-phase DECT imaging was carried out 1 h before 24 after embolism coronary. Color-coded maps used evaluate deficits 17-segment model. Myocardial MI induction for final comparison post-mortem histology.We...
The aim of this study was to investigate the effect three-dimensional (3D) bio-printed constructs consisting human umbilical-derived mesenchymal stem cells (HUMSCs) on cell viability, proliferation and differentiation in vitro. Functional 3D microspheres HUMSCs were constructed using electrostatic inkjet technique. parameters used for synthesis tissue first optimized. cultured assessed. results scanning electron microscopy (SEM) showed that isolated exhibited fibroblast-like spindle adherent...
Objective To investigate the effect of glioma associated oncogene 1 (GLI1) reduction by small interference RNAs (siRNA) on cell proliferation and glucose metabolism U87 cells. Methods siRNA for GLI1 was transfected into cells to inhibit expression GLI1. The non-transfected with non-sense siRNA were used as controls.The assessed MTT assay, cycle stage detected flow cytometry. Results The results Western blotting showed that GLI1’s significantly down-regulated in group compared...
Objective To investigate the regulative effect of microRNA-338-3p (miR-338-3p) on proliferation and apoptosis colorectal carcinoma (CRC) cells.Methods The lentiviral vector pLV-THM-miR-338-3p pLV-THM-miR-338-3p-inhibitor were constructed following protocols recommended by commercial corporation.The supernatant containing lentivirus particles was harvested to determine viral titer,and this then used transduce CRC-derived cell line,SW-620.Flow cytometry utilized for sorting green fluorescent...
Objective To construct a lentiviral expression vector of has-miR-338-3p-inhibitor and verify its influence on migration ability colorectal carcinoma cells.Methods The miR-338-3p inhibitor sequence was synthesized artificially inserted into pLV-THM plasmid consequently.The recombinant pLV-THM-miR-338-3p-inhibitor confirmed by restriction endonuclease analysis DNA sequencing.HEK-293T cells were co-transfected with lentivirus pLV-THM-miR-338-3p-inhibitor,psPAX2 pMD2.G.The supernatant containing...
To investigate the effects of Qihong capsule (QH) on HeLa cells infected by coxsackievirus B3 (CVB3) in vitro and its potential antiviral mechanism.HeLa were CVB3 vitro. XTT assay plaque inhibition performed to determine 50 % effective dose, (ED50), inhibitory concentration (IC50), 50% cytotoxicity (CC50) QH control drug, ribavirin. The total therapeutic index (TI) was calculated. Anti-viral time-course experiments compare anti-viral at different time points. attachment penetration also...