A5101749147- SARS-CoV-2 and COVID-19 Research
- Rheumatoid Arthritis Research and Therapies
- Gastric Cancer Management and Outcomes
- Cytokine Signaling Pathways and Interactions
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- Inflammasome and immune disorders
- Metastasis and carcinoma case studies
- Extracellular vesicles in disease
- Autoimmune and Inflammatory Disorders Research
- Galectins and Cancer Biology
- Metabolism, Diabetes, and Cancer
- Cancer Immunotherapy and Biomarkers
- Cell death mechanisms and regulation
- NF-κB Signaling Pathways
- Monoclonal and Polyclonal Antibodies Research
- HER2/EGFR in Cancer Research
- Calcium signaling and nucleotide metabolism
- Systemic Lupus Erythematosus Research
- Reproductive System and Pregnancy
- Cerebrovascular and Carotid Artery Diseases
- interferon and immune responses
- Chronic Lymphocytic Leukemia Research
- Inflammatory Myopathies and Dermatomyositis
Academy of Military Medical Sciences
2021-2025
Shanghai University
2019-2024
Institute of Microbiology
2024
Sichuan University
2015-2024
West China Hospital of Sichuan University
2015-2024
Hohai University
2016-2024
Institute of Microbiology
2018-2024
Southern Medical University
2018-2024
Nanfang Hospital
2018-2024
Key Laboratory of Guangdong Province
2018-2023
Antibody-dependent enhancement (ADE) of viral entry has been a major concern for epidemiology, vaccine development, and antibody-based drug therapy. However, the molecular mechanism behind ADE is still elusive. Coronavirus spike protein mediates into cells by first binding to receptor on host cell surface then fusing membranes. In this study, we investigated how neutralizing monoclonal antibody (MAb), which targets receptor-binding domain (RBD) Middle East respiratory syndrome (MERS)...
The pathogenesis of highly pathogenic Middle East respiratory syndrome coronavirus (MERS-CoV) remains poorly understood. In a previous study, we established an hDPP4-transgenic (hDPP4-Tg) mouse model in which MERS-CoV infection causes severe acute failure and high mortality accompanied by elevated secretion cytokines chemokines. Since excessive complement activation is important factor that contributes to lung injury after viral infection, this investigated the role MERS-CoV-induced damage....
Abstract There is an urgent need for animal models to study SARS-CoV-2 pathogenicity. Here, we generate and characterize a novel mouse-adapted strain, MASCp36, that causes severe respiratory symptoms, mortality. Our model exhibits age- gender-related mortality akin COVID-19. Deep sequencing identified three amino acid substitutions, N501Y, Q493H, K417N, at the receptor binding domain (RBD) of during in vivo passaging. All RBD mutations significantly enhance affinity its endogenous receptor,...
Therapeutic development is critical for preventing and treating continual MERS-CoV infections in humans camels. Because of their small size, nanobodies (Nbs) have advantages as antiviral therapeutics (e.g., high expression yield robustness storage transportation) also potential limitations low antigen-binding affinity fast renal clearance). Here, we developed novel Nbs that specifically target the receptor-binding domain (RBD) spike protein. They bind to a conserved site on RBD with...
Middle East respiratory syndrome coronavirus (MERS-CoV) is a highly pathogenic virus with crude mortality rate of ~35%. Previously, we established human DPP4 transgenic (hDPP4-Tg) mouse model in which studied complement overactivation-induced immunopathogenesis. Here, to better understand the pathogenesis MERS-CoV, role pyroptosis THP-1 cells and hDPP4 Tg mice MERS-CoV infection. We found that infection induced over-activation macrophages. The hDPP4-Tg infected overexpressed caspase-1 spleen...
Mixed lineage kinase domain-like (MLKL) is widely accepted as an executioner of necroptosis, in which MLKL mediates necroptotic signaling and triggers cell death a receptor-interacting protein 3 (RIPK3)-dependent manner. Recently, it increasingly noted that RIPK3 intrinsically silenced hepatocytes, raising question about the role hepatocellular carcinoma (HCC). This study reports previously unrecognized regulating parthanatos, programmed distinct from necroptosis. In HCC cells with intrinsic...
Abstract Depression is common in patients with rheumatoid arthritis (RA); however, the precise mechanisms underlying a link between depression and RA remain unclear. Accumulating evidence suggests role of gut–microbiota–brain axis depression. In this study, we investigated whether collagen-induced (CIA) mice produce depression-like behaviors abnormal composition gut microbiota. Furthermore, fecal microbiota transplantation (FMT) from causes phenotypes antibiotic cocktail (ABX)-treated mice....
Abstract BACKGROUND The differentiation between intracranial atherosclerotic stenosis (ICAS) and embolism as the immediate cause of acute ischemic stroke requiring endovascular therapy is important but challenging. In cases ICAS, we often observe a phenomenon call microcatheter “first-pass effect,” which temporary blood flow through occluded artery when angiographic initially advanced site total occlusion immediately retrieved proximally. OBJECTIVE To evaluate whether this first-pass effect...
Abstract In recent years, immunotherapy has emerged as a promising strategy for treating solid tumors, although its efficacy remains limited to subset of patients. Transforming non-responsive “cold” tumor types into immuno-responsive “hot” ones is critical enhance the immune-based cancer treatments. Pyroptosis, programmed cell death mechanism, not only effectively eliminates cells but also triggers potent inflammatory response initiate anti-tumor immune activities. This sheds light on...
Introduction: The global headlines have been dominated by the sudden and widespread outbreak of monkeypox, a rare endemic zoonotic disease caused monkeypox virus (MPXV). Genomic composition based machine learning (ML) methods recently shown promise in identifying host adaptability evolutionary patterns virus. Our study aimed to analyze genomic characteristics MPXV using ML methods. Methods: open reading frame (ORF) regions full-length genomes were filtered 165 ORFs selected as clusters with...
Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by synovial inflammation, causing substantial disability and reducing life quality. While macrophages are widely appreciated as master regulator in the inflammatory response of RA, precise mechanisms underlying regulation proliferation inflammation RA-derived fibroblast-like synoviocytes (RA-FLS) remain elusive. Here, we provide extensive evidence to demonstrate that macrophage contributes RA microenvironment remodeling...
Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV), an infectious first reported in 2012, has a mortality rate greater than 35%. Therapeutic antibodies are key tools for preventing and treating MERS-CoV infection, but to date no such agents have been approved treatment of this virus. Nanobodies (Nbs) camelid heavy chain variable domains with properties distinct from those conventional antibody fragments. We generated two oligomeric Nbs by linking or three monomeric (Mono-Nbs)...
COVID-19, caused by SARS-CoV-2 virus, has emerged as a global threat to human health. The main protease (Mpro) of is an excellent target for the development antiviral drugs against and various biosensors have been developed evaluate anti-coronavirus drugs. However, application these was limited due high background fluorescence, poor signal-to-noise ratios, constraints in enzyme activity thresholds accessing live viruses. In this study, we rationally designed highly conserved Mpro cleavage...