A5055053314- Reproductive System and Pregnancy
- Cancer-related molecular mechanisms research
- Pregnancy and preeclampsia studies
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Neonatal Respiratory Health Research
- Preterm Birth and Chorioamnionitis
- Quantum Mechanics and Applications
- Meningioma and schwannoma management
- Facial Nerve Paralysis Treatment and Research
- Cancer-related Molecular Pathways
- Nanoplatforms for cancer theranostics
- MicroRNA in disease regulation
- Maternal and fetal healthcare
- Statistical Mechanics and Entropy
- Quantum Computing Algorithms and Architecture
- Advanced Nanomaterials in Catalysis
- Pluripotent Stem Cells Research
- Nitric Oxide and Endothelin Effects
- CRISPR and Genetic Engineering
- Neurofibromatosis and Schwannoma Cases
- Pregnancy-related medical research
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- RNA Research and Splicing
Guangzhou Medical University
2021-2025
East China Normal University
2025
Eighth Affiliated Hospital of Sun Yat-sen University
2019-2024
Sun Yat-sen University
2019-2024
Southern Medical University
2014-2024
Beijing University of Chemical Technology
2024
Tsinghua University
2023
Cornell University
2023
Anhui Agricultural University
2022
Huashan Hospital
2016-2018
Posttranscriptional modification of tumor-associated factors plays a pivotal role in breast cancer progression. However, the underlying mechanism remains unknown. M6A modifications cells are dynamic and reversible have been found to impact tumor initiation progression through various mechanisms. In this study, we explored regulatory cell proliferation metabolism m6A methylation Hippo pathway. METHODS: A combination MeRIP-seq, RNA-seq metabolomics-seq was utilized reveal map tissues cells. We...
MicroRNA-381 (miR-381) has been reported to play suppressive or promoting roles in different malignancies. However, the expression level, biological function, and underlying mechanisms of miR-381 gastric cancer remain poorly understood. Our previous study indicated that transmembrane protein 16A (TMEM16A) contributed migration invasion predicted poor prognosis. In this study, we found inhibited metastasis through targeting TMEM16A expression. MiR-381 was analyzed using bioinformatic software...
Therapeutic development is critical for preventing and treating continual MERS-CoV infections in humans camels. Because of their small size, nanobodies (Nbs) have advantages as antiviral therapeutics (e.g., high expression yield robustness storage transportation) also potential limitations low antigen-binding affinity fast renal clearance). Here, we developed novel Nbs that specifically target the receptor-binding domain (RBD) spike protein. They bind to a conserved site on RBD with...
Abstract The metabolic switch from oxidative phosphorylation to glycolysis is required for tumorigenesis in order provide cancer cells with energy and substrates of biosynthesis. Therefore, it important elucidate mechanisms controlling the switch. MTR4 a RNA helicase associated nuclear exosome that plays key roles processing surveillance. We demonstrate frequently overexpressed hepatocellular carcinoma (HCC) an independent diagnostic marker predicting poor prognosis HCC patients. drives...
Abstract Background The transition of the myometrium from a quiescent to contractile state during labour is known involve inflammation, which characterized by infiltration immune cells and secretion cytokines. However, specific cellular mechanisms underlying inflammation in human parturition are not yet fully understood. Methods Through analysis transcriptomics, proteomics, cytokine arrays, was revealed. By performing single‐cell RNA sequencing (scRNA‐seq) spatiotemporal transcriptomic (ST)...
Objectives Oral and maxillofacial diseases affect approximately 3.5 billion people worldwide. With the continuous advancement of Artificial Intelligence technologies, particularly application generative pre-trained transformers like ChatGPT-4, there is potential to enhance public awareness prevention early detection these diseases. This study evaluated performance ChatGPT-4 in addressing oral disease questions using standard approaches Chain Thought (CoT) method, aiming gain a deeper...
One of the malignant transformation hallmarks is metabolism reprogramming, which plays a critical role in biosynthetic needs unchecked proliferation, abrogating cell death programs, and immunologic escape. However, mechanism metabolic switch not fully understood. Here, we found that S-nitrosoproteomic profile endogenous nitrogen oxide ovarian cancer cells targeted multiple components processes. Phosphofructokinase (PFKM), one most important regulatory enzymes glycolysis, was S-nitrosylated...
Human embryonic stem cells (hESCs) depend on glycolysis for energy and substrates biosynthesis.To understand the mechanisms governing metabolism of hESCs, we investigated transcriptional regulation glucose transporter 1 (GLUT1, SLC2A1), a key glycolytic gene to maintain pluripotency.By combining genome-wide data binding sites core pluripotency factors (SOX2, OCT4, NANOG, denoted SON), chromosomal interaction histone modification in identified potential enhancer GLUT1 (GE) element.GE...
Abstract Background Phosphatidylinositol-4-phosphate-binding protein GOLPH3L is overexpressed in human ductal carcinoma of the breast, and its expression levels correlate with prognosis breast cancer patients. However, roles tumorigenesis remain unclear. Methods We assessed biological function by combining bioinformatic prediction, metabolomics analysis RNA-seq to determine GOLPH3L-related pathways involved tumorigenesis. Dual-luciferase reporter assay coimmunoprecipitation (Co-IP) were used...
Abstract Background Placenta accreta spectrum disorders (PAS) are a severe complication characterized by abnormal trophoblast invasion into the myometrium. The underlying mechanisms of PAS involve complex interplay various cell types and molecular pathways. Despite its significance, both characteristics intricate this condition remain poorly understood. Methods Spatial transcriptomics (ST) single-cell RNA sequencing (scRNA-seq), were performed on tissue samples from four patients, including...
During labor, a variety of coordinated physiological and biochemical events cause the myometrium to transition from quiescent contractile state; molecular mechanisms responsible for this transition, however, remain unclear. To better understand at level, global transcriptome proteome human myometrial samples in labor those not were investigated through RNA sequencing (RNA-seq) quantitative liquid chromatography–tandem mass spectrometry (LC-MS/MS) via data-independent acquisition (DIA)...
To the Editor: Spontaneous preterm labor (PTL) causes infant morbidity and mortality. Regular uterine contractions were common characteristics in spontaneous at term or PTL. Thus, effective decrease suppression of myometrial contraction could prevent delivery. We discovered that microRNA-206 (miR-206) was a myometrium contraction-associated miRNA based on transcriptome data human laboring indicated miR-206 suppressed contractility by targeting junction protein alpha 1 (GJA1) would be new...
The E3 ligase HERC4 is overexpressed in human breast cancer and its expression levels correlated with the prognosis of patients. However, roles mammary tumorigenesis remain unclear. Here we demonstrate that knockdown cells dramatically suppressed their proliferation, survival, migration, tumor growth vivo, while overexpression promoted aggressive tumorigenic activities. a new for suppressor LATS1 destabilizes by promoting ubiquitination LATS1. miRNA-136-5p miRNA-1285-5p, which decreased...
Purpose: Micheliolide (MCL) is an effector compound of the flower which has been traditionally used to treat inflammation and cancer patients in oriental medicine. MCL killing effects on several immune cells by modulating apoptosis, cell cycle, metabolism. However, detail mechanisms anti-cancer activity remains be elucidated effect liver unknown. Methods: Cell proliferation was determined CCK8 clone formation assay. The xenograft model formed injecting Huh7 into NUDE mice evaluate vivo. We...
Abstract Uterine contraction is crucial for a successful labor and the prevention of postpartum hemorrhage. It enhanced by hypoxia; however, its underlying mechanisms are yet to be elucidated. In this study, transcriptomes revealed that hypoxia-inducible factor-1alpha was upregulated in laboring myometrial biopsies, while blockade decreased contractility myometrium myocytes vitro via small interfering RNA inhibitor, 2-methoxyestradiol. Chromatin immunoprecipitation sequencing directly binds...
UDP-GlcNAc is a sugar substrate necessary for the O-GlcNAcylation of proteins. SLC35B4 one nucleotide transporters that transport and UDP-xylose into endoplasmic reticulum Golgi apparatus glycosylation. The roles in hepatocellular carcinoma (HCC) tumorigenesis remain unknown. We find expression levels are higher HCC tissues than adjacent non-tumor tissues. important proliferation cells. Mechanistically, O-GlcNAc modification c-Myc thus stabilization c-Myc, which required tumorigenesis....
Micro RNAs (miRNAs) have been recognized as important regulators that are indispensable for testicular development and spermatogenesis. miRNAs endogenous transcriptomic elements mainly regulate the gene expression at post-transcriptional levels; however, key role of miRNA in bovine growth is not clearly understood. Thus, supposing to unveil transcriptomics changes developmental processes testes, we selected three immature calves sexually mature bulls local Wandong breed testicular-tissue...