Rat liver sinusoidal endothelial cells (LECs) express two hyaluronan (HA) receptors, of 175 and 300 kDa, responsible for the endocytic clearance HA. We have characterized eight monoclonal antibodies (mAbs) raised against 175-kDa HA receptor partially purified from rat LECs. These mAbs also cross-react with 300-kDa receptor. The is a single protein, whereas species contains three subunits, α, β, γ at 260, 230, 97 respectively (Zhou, B., Oka, J. A., Weigel, P. H. (1999) Biol. Chem. 274,...

DnaJ proteins act as essential molecular chaperones in protein homeostasis and complex stabilization under stress conditions. The roles of a tomato (Lycopersicon esculentum) chloroplast-targeted (LeCDJ1), whose expression was upregulated by treatment at 4 42 °C, with high light, NaCl, polyethylene glycol, H2O2, were investigated here using sense antisense transgenic tomatoes. plants exhibited not only higher chlorophyll content, fresh weight net photosynthetic rate, but also lower...

This study aimed to investigate the effects of body mass index (BMI) on infertility in women childbearing age.We performed a cross-sectional using data from 3624 participants National Health and Nutrition Examination Survey (NHANES). We used BMI fertility status survey as independent dependent variables, respectively. evaluated their relationship smoothed curve fitting multivariate logistic regression analysis well generalized additive model (GAM) determine effect BMI.Logistic linked after...

Murine light chain 3 (LC3) exists as two isoforms, LC3alpha and beta: LC3beta is an RNA-binding protein that enhances fibronectin (FN) mRNA translation, also a marker of autophagy. We report embryonic expression patterns for LC3beta, with some overlap but notable differences in the brain, tissues non-neuronal origin. knockout (-/-) mice develop normally without compensatory increase LC3alpha. LC3beta-/- fibroblasts (MEFs) exhibit reduced FN synthesis maintain wild type (WT) levels protein....

A novel single-cell analysis platform was fabricated using solid-state zinc-coadsorbed carbon quantum dot (ZnCQDs) nanocomposites as an electrochemiluminescence (ECL) probe for the detection of breast cancer cells and evaluation CD44 expression level. Solid-state ZnCQDs nanocomposite probes were constructed through attachment to gold nanoparticles then loading magnetic beads amplify ECL signal, exhibiting a remarkable 120-fold enhancement intensity. Hyaluronic acid (HA)-functionalized used...

A novel electrochemiluminescence (ECL) immunosensor based on the potential-resolved strategy was first developed for simultaneous determination of triple latent tuberculosis infection (LTBI) markers with high sensitivity, interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), and interleukin (IL)-2. In this work, luminol self-prepared carbon quantum dots CdS were integrated onto gold nanoparticles magnetic beads in sequence to fabricate ECL nanoprobes signal amplification....

ABSTRACT Tenascin-C is an extracellular matrix glycoprotein, the expression of which upregulated in remodeling arteries. In previous studies we showed that presence tenascin-C alters vascular smooth muscle cell shape and amplifies their proliferative response by promoting growth factor receptor clustering phosphorylation. Moreover, demonstrated denatured type I collagen induces protein production via β3 integrins. present study, examine pathway integrins stimulate tenascin-C, define a...

We recently purified the rat liver hyaluronan receptor for endocytosis (HARE) and found abundant expression of 175- approximately 300-kDa HARE species in sinusoidal endothelial cells liver, spleen, lymph nodes. report herein first cloning functional 175-kDa HARE. Peptide sequences were obtained from HARE, degenerate oligonucleotide primers designed reverse transcription-polymerase chain reaction cDNA cloning. Results 5'-rapid amplification ends, Northern analysis, N-terminal sequence,...

A mutiplex cytosensor based on a dual electrochemiluminescence (ECL) signal system was fabricated for in situ and simultaneous detection of the expression levels multiple cell-surface receptors, mannose epidermal growth factor receptor (EGFR), using luminol-capped gold nanoparticles (Au@luminol) CdS quantum dots (CdS QDs) as potential-resolved ECL nanoprobes. Two spatially resolved areas indium tin oxide (ITO) electrodes were modified with polyaniline (PANI) by electropolymerization, which...

Ziyuglycoside II is one of the major active compounds Sanguisorba officinalis L., which has a wide range clinical applications including hemostasis, antibiosis, anti-inflammation and anti-oxidation. This study investigated effect ziyuglycoside on growth human breast carcinoma MDA-MB-435 cells for first time. The results showed that could significantly inhibit through blocking cell cycle progression at G0/G1 S phase as well via inducing apoptosis. Accumulation reactive oxygen species (ROS)...

Osteoarthritis (OA) is a chronic degenerative joint disease. An increasing number of studies point to the role microRNAs (miRNAs or miRs) in pathogenesis OA. earlier study suggested that miR‑27b was associated with OA; however, precise mechanisms regarding involvement miR‑27 progression OA remain unclear. In present study, we first analyzed effects on vitro, degree expression decreased chondrocytes obtained from patients Transfection mimic increased viability CH8 cells and induced type-II...

The endocytic hyaluronan (HA) receptor of liver sinusoidal endothelial cells (LECs) is responsible for the clearance HA and other glycosaminoglycans from circulation in mammals. We report here first time purification this receptor. Using lectin immuno-affinity chromatography, two species were purified detergent-solubilized membranes prepared rat LECs. In nonreducing SDS-polyacrylamide gel electrophoresis (PAGE), these proteins migrated at 175- ∼300 kDa corresponding to previously identified...

Intimal cushions form in the fetal ductus arteriosus by fibronectin-dependent smooth muscle cell migration which is associated with greater efficiency of fibronectin mRNA translation. We investigated whether AU-rich element (ARE), UUAUUUAU, 3'-untranslated region (3'UTR) involved this mechanism transfecting cells plasmids containing chloramphenicol acetyltransferase coding its 3'UTR replaced bearing intact or mutated ARE. More efficient translation fusion versus ARE was observed. This effect...

Nucleolin plays important roles in a variety of cellular processes. In this study, we aimed to investigate the role nucleolin cardiac ischaemia–reperfusion (I–R) injury. We investigated expression pattern hearts subjected I–R, or neonatal rat cardiomyocytes hypoxia–re-oxygenation. found that was significantly down-regulated and cleaved protein present, both vivo vitro. Gene transfection RNA interference approaches were employed function nucleolin. Over-expression cytoprotective, whereas...

Latent tuberculosis infection (LTBI) is one of the major contributing factors for high incidence tuberculosis, and low contents LTBI markers in human serum present a great challenge diagnosis LTBI. Here, we reported novel electrochemiluminescence (ECL)-sensing platform precise analysis multiple markers, interferon-gamma (IFN-γ) interleukin (IL)-2. In this approach, self-prepared carbon quantum dots (CQDs) luminol were integrated onto gold nanoparticles (AuNPs), which further enriched on...

Snakebite incidence in southwestern China is mainly attributed to one of the several venomous snakes found country, white-lipped green pit viper Trimeresurus albolabris. Since antivenom produced from horses may cause numerous clinical side effects, present study was conducted aiming develop an alternative antibody (immunoglobulin Y - IgY) leghorn chickens.IgY egg yolk white chicken previously injected with T. albolabris venom extracted by water, precipitated ammonium sulfate and purified...

Constitutive heat shock protein 70 (Hsc70) is a molecular chaperone that has been shown to protect cardiomyocytes against oxidative stress. However, the mechanism responsible for this protection remains uncertain. To understand associated with myocardial protective role of Hsc70, we have embarked upon systematic search Hsc70-interacting proteins. Using adenosine diphosphate (ADP) affinity chromatography and mass spectrometry, identified α-enolase, rate-limiting enzyme in glycolysis, as novel...

Abstract —Our previous studies suggested that enhanced fibronectin mRNA translation in ductus arteriosus compared with aortic smooth muscle cells is related to increased expression of light chain 3 (LC3) microtubule-associated protein 1, which binds an AU-rich element the 3′ untranslated region mRNA. We therefore hypothesized microtubules are involved LC3-mediated translational regulation. In this study we show disruption by colchicine inhibits cultured cells. proposed mechanism might be...