A5110605684- Pain Mechanisms and Treatments
- Cytokine Signaling Pathways and Interactions
- Cancer Research and Treatments
- Virus-based gene therapy research
- Ubiquitin and proteasome pathways
- Ovarian cancer diagnosis and treatment
- Cancer Genomics and Diagnostics
- interferon and immune responses
- NF-κB Signaling Pathways
- Cell death mechanisms and regulation
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related gene regulation
- PARP inhibition in cancer therapy
- Neuroscience and Neuropharmacology Research
- TGF-β signaling in diseases
- Retinoids in leukemia and cellular processes
- Stress Responses and Cortisol
- RNA modifications and cancer
- Hormonal and reproductive studies
- Head and Neck Cancer Studies
- Cancer Mechanisms and Therapy
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- 14-3-3 protein interactions
- Neuropeptides and Animal Physiology
Riverside Hospital of Guangxi Zhuang Autonomous Region
2024
Zhujiang Hospital
2024
Southern Medical University
2024
Dana-Farber Cancer Institute
2011-2022
Nanchang University
2021-2022
The First Affiliated Hospital, Sun Yat-sen University
2021-2022
Sun Yat-sen University
2021-2022
Jinan University
2017-2021
Huazhong University of Science and Technology
2019-2021
Central Hospital of Wuhan
2019-2021
Field cancerization predisposes the upper aerodigestive tract mucosa to formation of multiple primary tumors, when exposed environmental carcinogens. Up-regulation epidermal growth factor receptor occurs early in squamous cell carcinogenesis and is critical for loss control a variety human cancers, including head neck carcinomas. In these tumor cells culture, stimulation initiates signaling via persistent activation selective STAT proteins. To determine timing Stat3 carcinogenesis, we...
Stimulation of epidermal growth factor receptor (EGFR) by ligand(s) leads to activation signaling molecules including Stat1 and Stat3, two members the signal transducers activators transcription (STAT) protein family. Activation Stat3 was constitutive in transformed squamous epithelial cells, which produce elevated levels TGF-alpha, enhanced addition exogenous TGF-alpha. Targeting using antisense oligonucleotides directed against translation initiation site, resulted significant inhibition....
High-grade serous ovarian cancer is characterized by extensive copy number alterations, among which the amplification of MYC oncogene occurs in nearly half tumors. We demonstrate that cells highly depend on for maintaining their oncogenic growth, indicating as a therapeutic target this difficult-to-treat malignancy. However, targeting directly has proven difficult. screen small molecules transcriptional and epigenetic regulation, find THZ1 - chemical inhibiting CDK7, CDK12, CDK13 markedly...
Abstract Purpose: Ovarian cancer is the leading cause of death from gynecologic malignancy in United States, with high rates recurrence and eventual resistance to cytotoxic chemotherapy. Model systems that allow for accurate reproducible target discovery validation are needed support further drug development this disease. Experimental Design: Clinically annotated patient-derived xenograft (PDX) models were generated tumor cells isolated ascites or pleural fluid patients undergoing clinical...
Central glucocorticoid receptors (GRs) and NMDA (NMDARs) have been shown to play a significant role in the mechanisms of neuropathic pain after peripheral nerve injury; however, how central GRs NMDARs interact this process remains unknown. Here we show that expression function spinal injury were modulated by GRs. Chronic constriction (CCI) rats induced time-dependent upregulation NR1 NR2 subunits NMDAR within cord dorsal horn ipsilateral CCI. The was significantly diminished intrathecal...
Peripheral glucocorticoid receptors (GRs) play a significant role in the anti-inflammatory effects of glucocorticoids; however, central GRs nociceptive behaviors after peripheral nerve injury (neuropathic pain behaviors) remains unknown. Here we show that development neuropathic (thermal hyperalgesia and mechanical allodynia) induced by chronic constriction (CCI) rats was attenuated either GR antagonist RU38486 (4 = 2 > 1 0.5 μg) or antisense oligonucleotide administered intrathecally...
Abstract Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKI) are the standard-of-care treatment for EGFR-mutant non–small cell lung cancers (NSCLC). However, most patients develop acquired drug resistance to EGFR TKIs. HER3 is a unique pseudokinase member of ERBB family that functions by dimerizing with other members (EGFR and HER2) frequently overexpressed in NSCLC. Although TKI mechanisms do not lead alterations HER3, we hypothesized targeting might improve efficacy...
Abstract Objectives Upregulation of epidermal growth factor receptor (EGFR) is critical for the loss control in a variety human cancers including squamous cell head and neck (SCCHN). In these tumor cells culture, EGFR stimulation initiates signaling via persistent activation STAT proteins, particularly Stat3. The present study was conducted to association between constitutive Stat3 SCCHN vivo investigate proliferative apoptotic consequences downmodulation vitro. Methods xenografts were...
Interruption of an autocrine growth pathway involving TGF-α and EGFR may inhibit tumor improve survival in head neck cancer patients. We previously demonstrated that biopsy specimens established cell lines from patients with squamous carcinoma the (SCCHN) overexpress its receptor, epidermal factor receptor (EGFR) at both mRNA protein levels. Protein localization studies showed are produced by same epithelial cells tissues further supporting a role for this ligand-receptor pair pathway. To...
Spinal NMDA receptor (NMDAR), protein kinase C (PKC), and glucocorticoid (GR) have all been implicated in the mechanisms of morphine tolerance; however, how these cellular elements interact after chronic exposure remains unclear. Here we show that expression spinal NMDAR PKCγ is regulated by GR through a cAMP response element-binding (CREB)-dependent pathway. Chronic (10 μg, i.t.; twice daily for 6 d) induced time-dependent upregulation GR, NR1 subunit NMDAR, within rat's cord dorsal horn....
Genomic alterations of the epidermal growth factor receptor (EGFR) gene play a crucial role in pathogenesis glioblastoma multiforme (GBM). By systematic analysis GBM genomic data, we have identified and characterized novel exon 27 deletion mutation occurring within EGFR carboxyl-terminus domain (CTD), addition to identifying additional examples previously reported mutations this region. We show that GBM-derived CTD mutants are able induce cellular transformation vitro vivo absence ligand...
Abstract Background Pregnant women experience physical, physiological, and mental changes. Health-related quality of life (HRQoL) is a relevant indicator psychological physical behaviours, changing over the course pregnancy. This study aims to assess HRQoL pregnant during different stages Methods cross-sectional was performed using The EuroQoL Group’s five-dimension five-level questionnaire (EQ-5D-5L) women, demographic data were collected. conducted in regional university hospital...
Spinal glutamate transporters (GT) have been implicated in the mechanisms of neuropathic pain; however, how spinal GT uptake activity is regulated remains unclear. Here we show that alteration arachidonic acid (AA) turnover after peripheral nerve injury regional and homeostasis. Chronic constriction (CCI) rats significantly reduced ((3)H-glutamate uptake) with an associated increase extracellular AA concentration from microdialysates on postoperative day 8. AACOCF3 (a cytosolic phospholipase...
Accumulating evidence has elucidated the biological function of lncRNAs in various tumors. FGD5 antisense RNA 1 (FGD5-AS1) is identified as a significant tumor regulator malignancies. Up to now, detailed FGD5-AS1 cervical cancer and its underlying molecular mechanisms remain uninvestigated. Bone marrow stromal cell antigen 2 (BST2) can play critical roles immune response, BST2 was explored currently. The level detected by qRT-PCR cells. expression significantly upregulated Then, decrease...
Management of pain after burn injury is an unresolved clinical issue. In a rat model hindpaw injury, we examined the effects systemic morphine on nociceptive behaviors following injury. Injury was induced by immersing dorsal part one into hot water bath (85 degrees C) for 4, 7, or 12 s under pentobarbital anesthesia. Mechanical allodynia to von Frey filament stimulation and thermal hyperalgesia radiant heat were assessed. Burn 12-s (but not 4-, 7-s) immersion resulted in reliable lasting...
Opioid analgesic tolerance is a pharmacological phenomenon that overtime diminishes the opioid effect. However, it remains unknown as to whether previous exposure would have long-term influence on upon subsequent administration. Here, we show onset and degree of antinociceptive cycle morphine were substantially exacerbated in rats made tolerant then recovered from administration, indicating development tolerance. Mechanistically, induced cyclic AMP protein kinase A-dependent upregulation...
Opioid analgesic tolerance is a pharmacologic phenomenon involving the mechanisms of cellular adaptation. Central glucocorticoid receptors (GRs) have been implicated in mechanism neuronal plasticity that has many steps common with opioid tolerance. In rat model morphine tolerance, authors examined hypothesis spinal GRs would play significant role development to antinociceptive effect morphine.In experiment 1, each group rats received GR antagonist RU38486 (0.5 or 1 microg), mineralocorticoid...
Abstract Ovarian cancer is a highly lethal in females. Therefore, it necessary to explore effective biomarkers for the diagnosis and prognosis of disease. Stratifin (SFN) cell cycle checkpoint protein that has been reported be involved oncogenesis. Our studies detected expression SFN ovarian by Oncomine, Human Protein Atlas database ULCAN database. Meanwhile, we found its coexpression gene cBioPortal online tool validated their different cells western blot reverse transcription quantitative...