A5024950554- Fusion materials and technologies
- Magnetic confinement fusion research
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Superconducting Materials and Applications
- Advanced biosensing and bioanalysis techniques
- Nuclear Materials and Properties
- Laser-Plasma Interactions and Diagnostics
- Epigenetics and DNA Methylation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Intraperitoneal and Appendiceal Malignancies
- Particle accelerators and beam dynamics
- Aortic Disease and Treatment Approaches
- RNA modifications and cancer
- Semiconductor Quantum Structures and Devices
- Tracheal and airway disorders
- Viral-associated cancers and disorders
- SARS-CoV-2 and COVID-19 Research
- Cardiac tumors and thrombi
- Nuclear reactor physics and engineering
- Cancer-related molecular mechanisms research
- Plant Molecular Biology Research
- Histone Deacetylase Inhibitors Research
- Pluripotent Stem Cells Research
- Plasma Diagnostics and Applications
Chiba University
2020-2024
The University of Tokyo
2010-2023
Molecular Oncology (United States)
2020-2023
Gunma Prefectural Cardiovascular Center
2023
Chiba University Hospital
2022-2023
Institute of Electrical and Electronics Engineers
2021
Gorgias Press (United States)
2020
Mitsubishi Electric (Germany)
2014
University of Tsukuba
1989-2013
Al-Azhar University
2010
The RNA-guided endonuclease Cas9 cleaves its target DNA and is a powerful genome-editing tool. However, the widely used Streptococcus pyogenes enzyme (SpCas9) requires an NGG protospacer adjacent motif (PAM) for recognition, thereby restricting targetable genomic loci. Here, we report rationally engineered SpCas9 variant (SpCas9-NG) that can recognize relaxed NG PAMs. crystal structure revealed loss of base-specific interaction with third nucleobase compensated by newly introduced...
Abstract The emergence of the Omicron variant SARS-CoV-2 is an urgent global health concern 1 . In this study, our statistical modelling suggests that has spread more rapidly than Delta in several countries including South Africa. Cell culture experiments showed to be less fusogenic and ancestral strain SARS-CoV-2. Although spike (S) protein efficiently cleaved into two subunits, which facilitates cell–cell fusion 2,3 , S was compared proteins Furthermore, a hamster model, decreased lung...
Soon after the emergence and global spread of SARS-CoV-2 Omicron lineage BA.1, another lineage, BA.2, began outcompeting BA.1. The results statistical analysis showed that effective reproduction number BA.2 is 1.4-fold higher than Neutralization experiments revealed immunity induced by COVID vaccines widely administered to human populations not against similar antigenicity notably different from Cell culture spike confers replication efficacy in nasal epithelial cells more efficient...
This paper presents a novel background subtraction method for detecting foreground objects in dynamic scenes involving swaying trees and fluttering flags. Most methods proposed so far adjust the permissible range of image variations according to training samples images. Thus, detection sensitivity decreases at those pixels having wide ranges. If we can narrow ranges by analyzing input images, be improved. For this narrowing, employ property that neighboring blocks have strong correlation,...
Abstract Soon after the emergence and global spread of a new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron lineage, BA.1 (ref 1, ), another BA.2, has initiated outcompeting BA.1. Statistical analysis shows that effective reproduction number BA.2 is 1.4-fold higher than Neutralisation experiments show vaccine-induced humoral immunity fails to function against like BA.1, notably, antigenicity different from Cell culture more replicative in human nasal epithelial cells...
Abstract Base editors derived from CRISPR-Cas9 systems and DNA editing enzymes offer an unprecedented opportunity for the precise modification of genes, but have yet to be used at a genome-scale throughput. Here, we test ability Target-AID base editor systematically modify genes genome-wide by targeting yeast essential genes. We mutate around 17,000 individual sites in parallel across more than 1500 identify over 700 which mutations significant impact on fitness. Using previously determined...
We have recently revealed that the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Mu variant shows a pronounced resistance to antibodies elicited by natural SARS-CoV-2 infection and vaccination.However, it remains unclear which mutations determine of antiviral sera. In addition, is how induces immunity.In this study, we reveal in spike protein, YY144-145TSN E484K, are responsible for disease 2019 convalescent sera during early 2020 vaccine sera.It notable Mu-infected...
An experiment was made to investigate the heat transfer a liquid drop impinging on hot surface. The transient temperature of heater surface measured by thin-film thermometer. fell contact immediately after with drop. increased increasing initial T0. In case water drop, however, it approximately constant for 200°C ≲ T0 300°C; and again ≳ 300°C. at turning point, i.e., ∼ 300°C, roughly coincided Leidenfrost point.
Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV)-associated malignant epithelial tumor endemic to Southern China and Southeast Asia. While previous studies have revealed a low frequency of gene mutations in NPC, its epigenomic aberrations are not fully elucidated apart from DNA hypermethylation. Epigenomic rewiring enhancer dysregulation, such as hijacking due genomic structural changes or extrachromosomal DNA, drive cancer progression.
Background subtraction is a useful and effective method for detecting moving objects in video images. Since this assumes that image variations are caused only by (i.e., the background scene assumed to be stationary), however, its applicability limited. In paper, we propose robustly handles various changes background. The learns chronological observed scene's terms of distributions vectors. operates evaluating Mahalanobis distances between averages such vectors newly herein expresses actual...
ROBO1 is a receptor mediating Slit‐induced repulsive action on axon guidance and differentially expressed in human cancers. Although ectodomain has been detected, the cleavage site had not determined. In this study we identified precise of ROBO1. We also report multi‐step proteolysis by metalloproteinases γ‐secretase, producing two carboxy‐terminal fragments, ROBO1‐CTF1 at 129‐kDa ROBO1‐CTF2 118‐kDa. have further demonstrated nuclear accumulation ROBO1, which abolished either...
Lung adenocarcinoma is classified morphologically into five histological subtypes according to the WHO classification. While each subtype correlates with a distinct prognosis, molecular basis has not been fully elucidated. Here we conducted DNA methylation analysis of 30 lung cases annotated predominant and three normal using Infinium BeadChip. Unsupervised hierarchical clustering revealed subgroups different levels: high-, intermediate-, low-methylation epigenotypes (HME, IME, LME)....
<b><i>Introduction:</i></b> Pseudomyxoma peritonei (PMP) is a disease characterized by progressive accumulation of intraperitoneal mucinous ascites produced neoplasms in the abdominal cavity. Since prognosis patients with PMP remains unsatisfactory, development effective therapeutic drug(s) matter pressing concern. Genetic analyses have clarified frequent activation <i>GNAS</i> and/or <i>KRAS</i>. However, involvement global epigenetic...
In JT-60U lower hybrid current drive (LHCD) experiments, a reversed magnetic shear configuration that was accompanied by internal transport barriers successfully maintained in quasi-steady state almost entirely non-inductively the LH driven and bootstrap current. The normalized β kept near 1 neutron emission rate nearly steady as well, indicating no accumulation of impurities plasma. Diagnostics showed profiles electron ion temperatures, density were unchanged during LHCD phase....
In the JT-60U tokamak a pre-formed reversed magnetic shear configuration was sustained non-inductively by means of lower hybrid current drive (LHCD). It formed neutral beam heating at build-up and had region extending to 65% minor radius. The for 7.5 s, extent kept as large 55% radius until very end. plasma stable against MHD activities. is also demonstrated that LHCD could modify keeping parameters, such toroidal field, same.
Heat flux to the material surfaces in scrape-off layer of a tokamak plasma is investigated experimentally. The time response heat measurement has been improved 0.2 ms by recently developed thin-film thermometer. result shows that calculated from simple sheath model including secondary electron emission effects even if epithermal electrons are present.
Abstract Ten‐eleven translocation 1 (TET1) is an essential methylcytosine dioxygenase of the DNA demethylation pathway. Despite its dysregulation being known to occur in human cancer, role TET1 remains poorly understood. In this study, we report that promotes cell growth liver cancer. The transcriptome analysis 68 clinical samples revealed a subgroup ‐upregulated hepatocellular carcinoma (HCC), demonstrating hepatoblast‐like gene expression signatures. We performed comprehensive cytosine...
DNA methylation accumulates in non-malignant gastric mucosa after exposure to pathogens. To elucidate how environmental, methylation, and lifestyle factors interplay influence primary neoplasia (GN) risk, we analyzed longitudinally monitored cohorts Japan Singapore.
A 38-year-old male was admitted to our hospital complaining of dysphagia, sore throat and fever. An upper GI series revealed a “double-barrelled” esophagus. Endoscopic examination the esophagus showed multiple ulcers mucosal bridges resulting from laceration submucosal layer. acute ulcer stomach also found. Fasting total parenteral nutrition for several weeks failed bring about any changes in endoscopic findings. As treatment, throughout were divided with diathermy knife under control. Soon...