A5084596571- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Viral-associated cancers and disorders
- Cancer Immunotherapy and Biomarkers
- Cytokine Signaling Pathways and Interactions
- Ubiquitin and proteasome pathways
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genomics and Chromatin Dynamics
- Gastric Cancer Management and Outcomes
- NF-κB Signaling Pathways
- Metastasis and carcinoma case studies
- vaccines and immunoinformatics approaches
- Invertebrate Immune Response Mechanisms
- Melanoma and MAPK Pathways
- Diet and metabolism studies
- Prostate Cancer Treatment and Research
- Lung Cancer Research Studies
- Gastrointestinal Tumor Research and Treatment
- Advanced Proteomics Techniques and Applications
- Acute Myeloid Leukemia Research
- Genetic Syndromes and Imprinting
- Colorectal Cancer Screening and Detection
Kanagawa Cancer Center
2023-2025
Kanagawa Prefectural Hospital Organization
2023-2025
Chiba University
2015-2022
University of Fukui
2011-2013
Japan Society for the Promotion of Science
2013
Extensive DNA methylation is observed in gastric cancer with Epstein-Barr virus (EBV) infection, and EBV infection the cause to induce this extensive hypermethylaton phenotype epithelial cells. However, some 5' regions of genes do not undergo de novo methylation, despite induction surrounding regions, suggesting existence a resistance factor against acquisition. We conducted an RNA-seq analysis cells without found that TET family genes, especially TET2, were repressed by at both mRNA protein...
To clarify molecular alterations in serrated pathway of colorectal cancer (CRC), we performed epigenetic and genetic analyses sessile adenoma/polyps (SSA/P), traditional adenomas (TSAs) high-methylation CRC. The methylation levels six Group-1 14 Group-2 markers, established our previous studies, were analyzed quantitatively using pyrosequencing. Subsequently, targeted exon sequencing 126 candidate driver genes examined that are associated with development. SSA/P showed high both frequent...
Primary prostate cancer (PC) progresses to castration-resistant PC (CRPC) under androgen deprivation therapy, by mechanisms e.g. expression of receptor (AR) splice variant-7 (AR-V7). Here we conducted comprehensive epigenome and transcriptome analyses comparing LNCaP, primary cells, LNCaP95, AR-V7-expressing CRPC cells derived from LNCaP. Of 399 AR-V7 target regions identified through ChIP-seq analysis, 377 could be commonly targeted hormone-stimulated AR, 22 were specifically AR-V7. Among...
The omentum is a common site of peritoneal metastasis in various cancers, including gastric cancer. It contains immune cell aggregates known as milky spots, which provide microenvironment for immunity by regulating innate and adaptive responses. In this study, we investigated gene expression profiles cells from omental spots patients with cancer (n = 37) RNA sequencing analysis classified the into four groups (G1-4). Notably, significant differences were observed between terms macroscopic...
Chimeric antigen receptor (CAR)-T cell therapy is an effective treatment for hematological cancers; however, challenges remain in its application to solid tumors. Among these, the control of CAR-T exhaustion important. The relationship between tonic signals generated by CAR self-activation and has attracted considerable attention. magnitude signal known depend on structure extracellular portion CAR, but role linker sequence single-chain variable region (scFv) function cells not been...
Accurately identifying neoantigens is crucial for developing effective cancer vaccines and improving tumor immunotherapy. Mass spectrometry-based immunopeptidomics has emerged as a promising approach to human leukocyte antigen (HLA) peptides presented on the surface of cells, but false-positive identifications remain significant challenge. In this study, liquid chromatography-tandem mass proteomics next-generation sequencing were utilized identify HLA-presenting neoantigenic resulting from...
Differences in gene expression between individual cells can be mediated by epigenetic regulation; thus, methods that enable detailed analyses of single are crucial to understanding this phenomenon. In study, genomic silencing regions Saccharomyces cerevisiae subject regulation, including the HMR, HML, and telomere regions, were investigated using a newly developed cell analysis method. This method uses fluorescently labeled proteins track changes over multiple generations cell. Epigenetic...
Epstein-Barr virus (EBV) is associated with particular forms of gastric cancer (GC). We previously showed that EBV infection into epithelial cells induced aberrant DNA hypermethylation in promoter regions and silencing tumor suppressor genes. here undertook integrated analyses transcriptome epigenome alteration during cells, to investigate activation enhancer related transcription factors (TFs) could contribute tumorigenesis. Formaldehyde-assisted isolation regulatory elements (FAIRE)...
Abstract Epstein‐Barr virus (EBV) is associated with approximately 10% of gastric cancers (GCs). We previously showed that EBV infection epithelial cells induces aberrant DNA methylation in promoter regions, which causes silencing critical tumor suppressor genes. Here, we analyzed gene expressions and active histone modifications (H3K4me3, H3K4me1, H3K27ac) genome‐widely EBV‐positive GC cell lines vitro EBV‐infected to elucidate the transcription factors contributing tumorigenesis through...
Abstract The selection of highly specific target antigens is critical for the development clinically efficient and safe chimeric antigen receptors (CARs). In search diagnostic marker malignant mesothelioma (MM), we have established SKM9‐2 monoclonal antibody (mAb) which recognizes a MM‐specific molecule, sialylated Protein HEG homolog 1 (HEG1), with high specificity sensitivity. this study, to develop novel therapeutic approach against MM, generated mAb‐derived CARs that included CD28...
Silenced chromatin domains are restricted to specific regions. Eukaryotic chromosomes organized into discrete delimited by domain boundaries. From approximately 6,000 genes in Saccharomyces cerevisiae, we previously isolated 55 boundary genes. In this study, focus on the molecular function of one genes, YCR076C/FUB1 (function boundary), whose has not been clearly defined vivo. Biochemical analysis Fub1p revealed that it interacted with multiple subunits 20S proteasome core particle (20S CP)....
Introduction: Clinical roles of plasma IL-6 levels have been reported in patients with various cancers, including non-small cell lung cancer (NSCLC), treated immune checkpoint inhibitors (ICIs). However, the other signaling components, soluble receptor (sIL-6R) and gp130 (sgp130), not elucidated. Methods: Blood was collected from 106 NSCLC before initiation ICI treatment (anti-PD-1 or anti-PD-L1 antibody). Plasma IL-6, sIL-6R, sgp130, their complexes were assessed by Cox regression hazard...
Neutrophil extracellular traps (NETs) released from neutrophils are related to cancer progression. However, the relationship between therapeutic effects of immune checkpoint inhibitors (ICIs) such as anti-PD-1 and anti-PD-L1 antibodies plasma NET concentration in patients with non-small cell lung (NSCLC) is poorly understood. In this study, concentrations citrullinated histone H3 (CitH3), a surrogate marker NETs, before/after treatment were examined advanced or recurrent NSCLC undergoing ICI...
Abstract Introduction Immune checkpoint inhibitors (ICI) have significantly improved the prognosis of non-small cell lung cancer (NSCLC). However, only a limited proportion patients can benefit from this therapy, and clinically useful predictive biomarkers remain to be elucidated. Methods Blood was collected 189 with NSCLC before six weeks after initiation ICI treatment (anti-PD-1 or anti-PD-L1 antibody). Soluble PD-1 (sPD-1) PD-L1 (sPD-L1) in plasma were analyzed for evaluation their...