A5027599882- Spinal Cord Injury Research
- Spinal Dysraphism and Malformations
- Pain Mechanisms and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Pluripotent Stem Cells Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Mesenchymal stem cell research
- Nerve Injury and Rehabilitation
- Pain Management and Treatment
- Family and Disability Support Research
- Neonatal and fetal brain pathology
- Automotive and Human Injury Biomechanics
- Spinal Fractures and Fixation Techniques
- Cytomegalovirus and herpesvirus research
- Traffic and Road Safety
- Anesthesia and Pain Management
- Muscle activation and electromyography studies
- Developmental Biology and Gene Regulation
- Stroke Rehabilitation and Recovery
- RNA Interference and Gene Delivery
- Transcranial Magnetic Stimulation Studies
- Reproductive System and Pregnancy
Swedish Medical Center
2021-2024
University of Minnesota
2023
Craig Hospital
2008-2021
Neurological Surgery
2021
University of Colorado Boulder
2011-2012
Georgetown University
1990
George Washington University
1990
University of Washington
1990
ABSTRACT Human cytomegalovirus (HCMV) is the most common cause of congenital infections in developed countries, with an incidence varying between 0.5 and 2.2% consequences from asymptomatic infection to lethal conditions for fetus. Infants that are at birth may still develop neurological sequelae, such as hearing loss mental retardation, a later age. Infection neural stem precursor cells by HCMV consequent disruption proliferation, differentiation, and/or migration these be primary mechanism...
Surgically created lesions of the spinal cord dorsal root entry zone (DREZ) to relieve central pain after injury (SCI) have historically resulted in modest outcomes. A review literature indicates that fair good relief is achieved approximately 50% patients when an empirical procedure performed. This study was undertaken determine if intramedullary electrical guidance DREZ lesioning could improve outcomes with SCI-induced pain. Additionally, data were used be somatotopically mapped regard...
Cystic lesions of the spinal cord (syringomyelia) may occur after injury. Posttraumatic syringomyelia result in a myelopathy causing symptoms sensory and motor loss, as well worsening spasticity, pain, hyperhidrosis, autonomic dysreflexia. Shunting cyst cavity along with untethering scarred is widely accepted treatment choice. However, long-term stabilization progressive caused by posttraumatic suboptimal because arachnoidal rescarring, shunt tube blockage, reexpansion. A new neurosurgical...
Object Permanent neurological loss after spinal cord injury (SCI) is a well-known phenomenon. There has also been growing recognition and improved understanding of the pathophysiological mechanisms late progressive loss, which may occur SCI as result posttraumatic tethering (SCT), myelomalacia, syringomyelia. A clinical study 404 patients sustaining traumatic SCIs undergoing surgery to arrest myelopathy caused by SCT, with or without myelomalacia cystic cavitation (syringomyelia) was...
Abstract Human cytomegalovirus (HCMV) is the most common cause of congenital infections in developed countries, with an incidence varying between 0.5–2.2%. Such infection may be consequence either a primary or reactivation latent mother and outcome vary from asymptomatic to severe brain disorders. Moreover, infants that are at time birth still develop neurologic sequelae later age. Our hypothesis stem cells central nervous system by HCMV alters proliferation, differentiation migration these...
Mechanical allodynia, the perception of innocuous tactile stimulation as painful, is a severe symptom chronic pain often produced by damage to peripheral nerves. Allodynia affects millions people and remains highly resistant classic analgesics therapies. Neural mechanisms for development maintenance allodynia have been investigated in spinal cord, brainstem, thalamus, forebrain, but manipulations these regions rarely produce lasting effects. We found that long-term alleviation allodynic...
Abstract In vitro expanded neural precursor cells (NPCs) may provide a stable source for cell therapy. search of the optimal spinal cord repair, we investigated influences gestational age, regional heterogeneity, and long‐term in propagation. The cellular content neurosphere cultures prior to after differentiation was studied by immunocytochemistry flow cytometry. Human forebrain NPCs deriving from first‐trimester tissue were cultured as neurospheres presence epidermal growth factor, basic...
Cell transplantation therapies for central nervous system (CNS) deficits such as spinal cord injury (SCI) have been shown to be effective in several animal models. One cell type that has transplanted is neural precursor cells (NPCs), which there are possible sources. We studied NPCs derived from human embryonic stem (hESCs) and fetal CNS tissue (hfNPCs), cultured neurospheres, the expression of pluripotency genes during induction vitro differentiation. mRNA markers Nanog, Oct-4, Gdf3, DNMT3b...
Studies in model organisms constitute the basis of our understanding principal molecular mechanisms cell fate determination developing central nervous system. Considering emergent applications stem cell-based regenerative medicine, it is important to demonstrate conservation subtype specific gene expression programs human as compared vertebrates. We have examined patterns key regulatory genes neural progenitor cells and their neuronal glial descendants spinal cord, hindbrain, midbrain, these...
Posttraumatic syringomyelia (PTS) is a serious condition of progressive expansion spinal cord cysts, affecting patients with injury years after injury. To evaluate neural cell therapy to prevent cyst and potentially replace lost neurons, we developed rat model PTS. We combined contusive trauma subarachnoid injections blood, causing tethering the surrounding vertebrae, resulting in chronically expanding cysts. The cysts were usually located rostral injury, extracanalicular, lined by...
Central neuropathic pain occurs with multiple sclerosis, stroke, and spinal cord injury (SCI). Models of SCI are commonly used to study central excellent at modeling gross physiological changes. Our goal was develop a rat model by traumatizing discrete region the dorsal cord, thereby avoiding issues including paralysis, urinary tract infection, autotomy. To this end, root avulsion pursued. The developed first determining number avulsed roots sufficient induce below-level hindpaw mechanical...
Culturing stem cells as free-floating aggregates in suspension facilitates large-scale production of closed systems, for clinical use. To comply with GMP standards, the use substances such proteolytic enzymes should be avoided. Instead enzymatic dissociation, growing cell may mechanically cut at passage, but available methods are not compatible and hence translation into clinic becomes a severe bottle-neck. We have developed Biogrid device, which consists an array micrometerscale knife...
Neuropathic pain in spinal cord injury (SCI) is associated with inflammation both the peripheral and central nervous system (CNS), which may contribute to initiation maintenance of persistent pain. An understanding factors contributing neuroinflammation lead new therapeutic targets for neuropathic Moreover, novel circulating biomarkers facilitate earlier more effective treatment. MicroRNAs (miRNAs) are short, non-coding single-stranded RNA that have emerged as important molecular mediators...
Over the past 3 years, during a time when seat belt use has increased, we have managed nine midlumbar spine fractures in children caused by standard rear lap belts. Children with injury typically complained of abdominal and back pain, nature their injuries was evident presence belt-shaped abrasion across lower abdomen. Midlumbar fracture may be associated paraplegia life-threatening visceral injury. If is analyzed as beam, full spectrum reported predictable. This study shows that belts...