Nucleotide-binding oligomerization domain-Like Receptor with a Pyrin domain 3 (NLRP3) inflammasome was emerged as marker of metabolic dysregulation. We revealed that age-related Sirtuin-1 (SIRT1) modulates cardiac metabolism medicated inflammatory response during ischemia and reperfusion (I/R) stress. hypothesize SIRT1 attenuates NLRP3 inflammasome-dependent inflammation pyroptosis myocardial I/R through modulation. C57BL/6J wild type (WT) mice, inducible cardiomyocyte specific knockout...

Abstract Sirtuin1 (SIRT1) and Sirtuin3 (SIRT3) protects cardiac function against ischemia/reperfusion (I/R) injury. Mitochondria are critical in response to myocardial I/R injury as disturbance of mitochondrial dynamics contributes dysfunction. It is hypothesized that SIRT1 SIRT3 components maintaining mitochondria homeostasis especially exert cardioprotective actions under stress. The results demonstrated deficiency aged (24–26 months) mice hearts led the exacerbated dysfunction terms...

Ischemic heart disease (IHD) is the leading cause of death, with age range being primary factor for development. The mechanisms by which aging increases vulnerability to ischemic insult are not well understood. We aim use single-cell RNA sequencing discover transcriptional differences in various cell types between aged and young mice, may contribute aged-related insult. Utilizing 10× Genomics Single-Cell sequencing, we were able complete bioinformatic analysis identity novel differential...

Abstract Background Despite the recently increased incidence and improved awareness of filler-induced ischemic injuries, arterioembolic behavior filler products has not been well described. Objectives To evaluate embolic varying dilutions Calcium Hydroxylapatite-Carboxymethylcellulose (CaHA-CMC) gel mixtures within an artificial macrovascular perfusion model proximal facial artery with correlation against published instances injuries in literature. Methods CaHA-CMC were assessed via...

Ischemia heart disease is the leading cause of death world-widely and has increased prevalence exacerbated myocardial infarction with aging. Sestrin2, a stress-inducible protein, declines aging in rescue Sestrin2 aged mouse improves resistance to ischemic insults caused by ischemia reperfusion. Here, through combination transcriptomic, physiological, histological, biochemical strategies, we found that deficiency shows an aged-like phenotype excessive oxidative stress, provoked immune...

APC (activated protein C) is a plasma serine protease with anticoagulant and anti-inflammatory activities. EPCR (Endothelial C receptor) associated APC's activity mediates its downstream signaling events. exerts cardioprotective effects during ischemia reperfusion (I/R). This study aims to characterize the role of APC-EPCR axis in ischemic insults aging.Young (3-4 months) aged (24-26 wild-type C57BL/6J mice, as well point mutation (EPCRR84A/R84A) knockin mice incapable interaction wild type...

A progressive defect in the energy generation pathway is implicated multiple aging-related diseases, including cardiovascular conditions and Alzheimer's Disease (AD). However, evidence of pathogenesis cardiac dysfunction AD associations between two organ diseases need further elucidation. This study aims to characterize cellular defects resulting decreased function AD-model. 5XFAD mice, a strain expressing five mutations human APP PS1 that shows robust Aβ production with visible plaques at 2...

Sestrin2 (Sesn2) is a stress-inducible protein that declines with aging in the heart. We reported rescue Sesn2 levels aged mouse hearts through gene therapy improves resistance of to ischemia and reperfusion (I/R) insults. hypothesize as scaffold maintains mitochondrial integrity protect heart from ischemic injury during I/R. Young C57BL/6 J (3–6 months), (24–26 young KO months, background) mice were subjected vivo regional reperfusion. The left ventricle was collected for transcriptomics,...

Obesity is of concern to the population because it known cause inflammation and oxidative stress throughout body, leading patient predisposition for health conditions such as diabetes, hypertension, some cancers. However, proteins that are activated in times may provide cytoprotective properties. In this study, we aim gain further understanding interconnection between Nrf2 Sesn2 during obesity-related how relationship can play a role cardio-protection. Cardiomyocyte-specific knockout...

Уфимский государственный нефтяной

Introduction: Metformin activates AMP-activated protein kinase (AMPK) to improve cardiac function during ischemia and reperfusion (I/R). We reported that Sestrin2 (Sesn2) is associated with AMPK maintains oxidative phosphorylation (OXPHOS) under I/R stress. The role of age-related Sesn2-AMPK signaling in the beneficial actions metformin on ischemic insults remains unknown. Hypothesis: mitochondrial integrity limits damage caused by through pathway. Methods: Young (3-6 months) aged (22-24...

Introduction: Activated protein C (APC) is a circulating protease with anti-coagulant property. Endothelial receptor (EPCR) mediates APC's downstream events. We have revealed that APC exerts cardioprotective effects during ischemia and reperfusion (I/R). Hypothesis: There an impaired signaling in aging administration of restores aged heart to improve cardiac tolerance ischemic insult caused by I/R stress. Methods: Young C57BL/6J (3-4 months), (24-26 EPCR R84A/R84A months, C57BL6/J...

Introduction: Sestrin2 (Sesn2) is a stress-inducible protein that declines with aging in the heart. We have reported rescue Sesn2 levels of aged mouse hearts through gene therapy improves resistance to ischemic insult caused by ischemia and reperfusion (I/R). Hypothesis: as scaffold maintains mitochondrial integrity protect heart from injury during I/R. Methods: Young C57BL/6J (3-6 months), (24-26 young Sesn2-KO months, background) mice were subjected 45 min regional 24 hours reperfusion....

For centuries, the library has embodied one of greatest intellectual achievements mankind. Determining horizon university research libraries and librarianship, as a rule, is considered in perspective their institutional development. The article considers problems business conditions formation information society Ukraine. analysis scientific base reveals that leading topics literature are issues material technical support libraries, financing industry, introduction innovative management...

Introduction: Obesity increases the chances of developing cardiovascular disease and strokes. The overabundance adipose tissue that occurs in obese individuals oxidative stress, which trigger upregulation antioxidant proteins, Sesn2 Nrf2. Hypothesis: Obesity-related stress triggers cardiac Nrf2 to function cardioprotective properties. Methods: Cardiomyocyte specific knock out (cSesn2 -/- ) overexpression (tTa-tet-Sesn2) C57BL/6J mice, their wildtype littermates (Sesn2 f/f tet-Sesn2) were...

Introduction: Sirtuin 1 (SIRT1) is a nicotinamide adenine dinucleotide-dependent histone deacetylase. We revealed that the pressure-overload-induced SIRT1 can exacerbate cardiac systolic dysfunction. But mechanisms by which undermines function under pathological conditions remain unclear. Hypothesis: induced hypotrophy disturbs mitochondrial dynamics homeostasis resulting in an exacerbated Methods: Wild-type littermates f/f (3-5 months) and inducible cardiomyocyte-specific knockout (icSIRT1...

Introduction: Activated protein C (APC) is a vitamin-K-dependent plasma serine protease that functions as natural anticoagulant to downregulate thrombin generation in the clotting cascade. Endothelial receptor (EPCR) mediates APC's cytoprotective properties. We found cardiac EPCR expression downregulated aging, leading intolerance ischemic insults. However, role of APC human heart under stress remains unclear. Hypothesis: APC-EPCR axis protects cardiomyocytes against hypoxia-reoxygenation...

Introduction Autoimmune diseases of central nervous system (CNS) are wide spread in children. In some cases, mental disturbances such patients barely noticeable the beginning, which hinders early detection risks child’s development. Objectives The study focuses on comparative analysis structure disorders pediatric with autoimmune CNS. Methods Research includes two cases: girls aged 14 and 16, one acute disseminated encephalomyelitis (ADEM), disease onset at 4 years 11 months, another...