A5044157407- Advanced X-ray Imaging Techniques
- Medical Imaging Techniques and Applications
- Advanced X-ray and CT Imaging
- Dental Radiography and Imaging
- Nanoplatforms for cancer theranostics
- Nuclear Physics and Applications
- Bone health and osteoporosis research
- Advanced Electron Microscopy Techniques and Applications
- Endodontics and Root Canal Treatments
- Cancer Research and Treatments
- Inhalation and Respiratory Drug Delivery
- Hedgehog Signaling Pathway Studies
- Epigenetics and DNA Methylation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Radiomics and Machine Learning in Medical Imaging
- TGF-β signaling in diseases
- Oral and Maxillofacial Pathology
- Advanced MRI Techniques and Applications
- Radiation Dose and Imaging
- dental development and anomalies
- Advanced Biosensing Techniques and Applications
- Effects of Vibration on Health
- Dental materials and restorations
- Bone health and treatments
- Histone Deacetylase Inhibitors Research
Elettra-Sincrotrone Trieste S.C.p.A.
2017-2025
University Hospital Heidelberg
2020-2025
Heidelberg University
2020-2025
University of Göttingen
2015-2025
Universitätsmedizin Göttingen
2015-2025
German Center for Lung Research
2021-2025
Max Planck Institute for Multidisciplinary Sciences
2022-2024
University Medical Center
2024
University Radiology
2006-2023
Max Planck Institute for the Study of Religious and Ethnic Diversity
2023
Rödig T, Hausdörfer Konietschke F, Dullin C, Hahn W, Hülsmann M. Efficacy of D‐RaCe and ProTaper Universal Retreatment NiTi instruments hand files in removing gutta‐percha from curved root canals – a micro‐computed tomography study. International Endodontic Journal , 45 580–589, 2012. Abstract Aim To compare the efficacy two rotary retreatment systems Hedström filling material canals. Methodology Curved 57 extracted teeth were prepared using FlexMaster filled with AH Plus. After...
To compare the efficacy of reciprocating and rotary NiTi-instruments in removing filling material from curved root canals using micro-computed tomography.Sixty were prepared filled with gutta-percha sealer. After determination canal curvatures radii two directions as well volumes material, teeth assigned to three comparable groups (n = 20). Retreatment was performed Reciproc, ProTaper Universal or Hedström files. Percentages residual dentine removal assessed micro-CT imaging. Working time...
To compare the efficacy of fiber post removal using conventional (CONV) versus guided endodontics (GE) in terms dentin loss, residual resin material, procedural errors, and working time vitro.
In this study, short-term, whole-body vertical vibration at 90 Hz improved trabecular bone quality. There was an improvement of quality and density in both osteoporotic control rats. This treatment may therefore be attractive option for the osteoporosis.Aside from pharmacological options, physical exercise is known to augment mass. effects (WBVV) on were evaluated using rat model.Sixty female Sprague Dawley rats ovariectomized (C) or sham (SHAM) operated age 3 months. After months, groups...
Patched (Ptch) heterozygous mice develop medulloblastoma (MB) and rhabdomyosarcoma (RMS) resembling the corresponding human tumors. We have previously shown that epigenetic silencing of intact Ptch allele contributes to tumor formation in this model. Here, we investigated whether targeting mechanisms could be useful treatment Ptch-associated cancers. A reduction endogenous DNA methyltransferase1 (Dnmt1) activity significantly reduced incidence knockout mice. combined with Dnmt inhibitor...
Activation of the Hedgehog (Hh)-signaling pathway due to deficiency in Hh receptor Patched1 (Ptch) is pivotal defect leading formation basal cell carcinoma (BCC). Recent reports provided evidence Ptch-dependent secretion vitamin D(3)-related compound, which functions as an endogenous inhibitor signaling by repressing activity signal transduction partner Ptch, Smoothened (Smo). This suggests that Ptch-deficient tumor cells are devoid this substance, turn results activation Hh-signaling. Here,...
Abstract We have performed x-ray phase-contrast tomography on mouse lung tissue. Using a divergent beam generated by nanoscale focusing, we used zoom to produce three-dimensional reconstructions with selectable magnification, resolution and field of view. Thus, macroscopic tissue samples extending over several mm can be studied in sub-cellular-level structural detail. The capability and, particular, the high dose efficiency are enabled near-perfect exit wavefront an optimized waveguide...
Synchrotron radiation micro-computed tomography (SRµCT) based virtual histology, in combination with dedicated ex vivo staining protocols and/or phase contrast, is an emerging technology that makes use of three-dimensional images to provide novel insights into the structure tissue samples at microscopic resolution short acquisition times order minutes or seconds. However, high dose creates special demands on sample preparation and staining. As a result lack specific it can supplement but not...
The small size of the adult and developing mouse heart poses a great challenge for imaging in preclinical research. aim study was to establish phosphotungstic acid (PTA) ex-vivo staining approach that efficiently enhances x-ray attenuation soft-tissue allow high resolution 3D visualization hearts by synchrotron radiation based μCT (SRμCT) classical μCT. We demonstrate SRμCT PTA stained allows cardiac atrium, ventricles, myocardium especially its fibre structure vessel walls detail...
Oesophageal cancer is a highly aggressive tumour entity with at present poor prognosis. Therefore, novel treatment options are urgently needed. Hyaluronan (HA) polysaccharide in the matrix of human oesophageal squamous cell carcinoma (ESCC). Importantly, vitro ESCC cells critically depend on HA synthesis to maintain proliferative phenotype. The aim study (1) HA-synthase (HAS) expression and regulation ESCC, (2) translate results into mouse xenograft model effects systemic versus targeted HAS...
This study investigated the effect of vibration on bone healing and muscle in intact ovariectomized rats. Thirty (at 3 months age) 30 5-month old female Sprague-Dawley rats underwent bilateral metaphyseal osteotomy tibia. Five days later, half were exposed to whole-body vertical (90 Hz, 0.5 mm, 4 x g acceleration) for 15 min twice a day during days. The other animals did not undergo vibration. After decapitation rats, one tibia was used computed tomographic, biomechanical, histological...
Numerous whole-body vibration (WBV) devices of various forces are available on the market, although their influence musculoskeletal system is not yet understood. The effect different WBVs bone healing and muscle function was evaluated in rats ovariectomized at 3 months age. 2 after ovariectomy, bilateral metaphyseal tibia osteotomy T-plate osteosynthesis were performed. Rats divided into groups: intact, OVX, OVX exposed to vertical 35, 50, 70, or 90 Hz (experiment 1) horizontal 30, 2) 5 days...
The seedling stage is the most susceptible one during a tree's life. Water relations may be crucial for seedlings due to their small roots, limited water buffers and effects of drought on transport. Despite obvious relevance, studies xylem hydraulics are scarce as respective methodical approaches limited. Micro-CT scans intact Acer pseudoplatanus Fagus sylvatica dehydrated different potentials (Ψ) allowed simultaneous observation gas-filled versus water-filled conduits calculation percentage...
We have performed high-resolution phase-contrast tomography on whole mice with a laboratory setup. Enabled by high-brilliance liquid-metal-jet source, we show the feasibility of propagation-based phase contrast in local even presence strongly absorbing surrounding tissue as it is case small animal imaging lung. demonstrate technique reconstructions mouse lung for two different fields view, covering organ, and zoom to finer structure terminal airways alveoli. With resolution few micrometers...
The aim of this study was to optimise the experimental protocol and data analysis for in-vivo breast cancer x-ray imaging. Results are presented experiment at SYRMEP beamline Elettra Synchrotron using propagation-based phase-contrast mammographic tomography method, which incorporates not only absorption, but also phase information. In images tissue samples, a size corresponding full human breast, with radiologically acceptable doses were obtained, degree improvement image quality (from...
Propagation-based X-ray phase-contrast computed tomography (PBI) has already proven its potential in a great variety of soft-tissue-related applications including lung imaging. However, the strong edge enhancement, caused by phase effects, often hampers image segmentation and therefore quantitative analysis data sets. Here, benefits applying single-distance retrieval prior to three-dimensional reconstruction (PhR) are discussed quantified compared with reconstructions conventional PBI sets...
Abstract The detection of tumours in an early phase tumour development combination with the knowledge expression markers such as epidermal growth factor receptor (EGFR) is important prerequisite for clinical decisions. In this study we applied anti-EGFR nanobody 99m Tc-D10 visualizing small lesions volumes below 100 mm 3 by targeting EGFR orthotopic human mammary MDA-MB-468 and MDA-MB-231 subcutaneous epidermoid A431 carcinoma mouse models. Use a size 15.5 kDa enables single photon emission...
Abstract Ontogenetic changes in skull shape and size are ubiquitous altricial vertebrates, but typically unidirectional minimal full-grown animals. Red-toothed shrews exhibit a rare exception, where the shape, mass of skull, brain, several major organs, show significant bidirectional seasonal changes. We now similar male-biased shrinking (16%) regrowth (8%) standardized braincase depth least weasels ( Mustela nivalis ). Juvenile also growth overshoot, followed by shrinkage period lasting...
Abstract The increasing number of patients with end stage chronic kidney disease not only calls for novel therapeutics but also pioneering research using convincing preclinical models and innovative analytical techniques. aim this study was to introduce a virtual histology approach micro computed tomography (µCT) the entire murine in order close gap between single slice planar 3D high resolution dataset. An ex vivo staining protocol based on phosphotungstic acid diffusion adapted enhance...