A5034420791- Autism Spectrum Disorder Research
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Neurological Disease Mechanisms and Treatments
- Genetics and Neurodevelopmental Disorders
- Child Nutrition and Feeding Issues
- Neurogenesis and neuroplasticity mechanisms
- Acute Ischemic Stroke Management
- Mitochondrial Function and Pathology
- Family and Disability Support Research
- Obsessive-Compulsive Spectrum Disorders
- Cerebrovascular and Carotid Artery Diseases
- Anesthesia and Neurotoxicity Research
- Traditional Chinese Medicine Analysis
- RNA regulation and disease
- S100 Proteins and Annexins
- Heavy Metal Exposure and Toxicity
- Circular RNAs in diseases
- Stress Responses and Cortisol
- Forensic and Genetic Research
Shaanxi Normal University
2013-2024
Ministry of Agriculture and Rural Affairs
2024
ZheJiang Academy of Agricultural Sciences
2024
Ministry of Education of the People's Republic of China
2018-2021
Eli Lilly (United States)
2016
University of California, Davis
2009-2013
University of California, San Francisco
2010-2012
Wake Forest University
2010-2011
Xi'an Jiaotong University
2004-2011
University of Cincinnati
2010-2011
MicroRNAs (miRNAs) regulate gene expression and have a critical role in many biologic pathologic processes. We hypothesized that miRNA profiles injured brain (hippocampus) would show common as well unique when compared with those of blood. Adult, untouched, control rats were sham surgeries, ischemic strokes, hemorrhage (lysed blood, fresh or thrombin), kainate-induced seizures. Brain whole-blood assessed 24 h later using TaqMan rodent arrays. MicroRNA response different for each condition....
A blood-based biomarker of acute ischemic stroke would be significant value in clinical practice. This study aimed to (1) replicate a larger cohort our previous using gene expression profiling predict stroke; and (2) refine prediction by including control groups relevant stroke.Patients with (n=70, 199 samples) were compared subjects who healthy (n=38), had vascular risk factors (n=52), myocardial infarction (n=17). Whole blood was drawn ≤3 hours, 5 24 hours after onset from subjects. RNA...
Abstract Objective: The cause of stroke remains unknown or cryptogenic in many patients. We sought to determine whether gene expression signatures blood can distinguish between cardioembolic and large‐vessel causes stroke, these profiles predict etiology the group. Methods: A total 194 samples from 76 acute ischemic patients were analyzed. RNA was isolated run on Affymetrix U133 Plus2.0 microarrays. Genes that determined at 3, 5, 24 hours following onset. Predictors evaluated using...
Mutations in the gene encoding leucine-rich repeat kinase 2 (LRRK2) can cause Parkinson's disease (PD), and most common disease-associated mutation, G2019S, increases activity. Because LRRK2 expression levels rise during synaptogenesis are highest dorsal striatal spiny projection neurons (SPNs), we tested hypothesis that LRRK2-G2019S mutation would alter development of excitatory synaptic networks striatum. To circumvent experimental confounds associated with overexpression, used mice...
Gene expression studies require appropriate normalization methods. One such method uses stably expressed reference genes. Since suitable genes appear to be unique for each tissue, we have identified an optimal set of the most in human blood that can used normalization.Whole-genome Affymetrix Human 2.0 Plus arrays were examined from 526 samples males and females ages 2 78, including control subjects patients with Tourette syndrome, stroke, migraine, muscular dystrophy, autism. The top 100 a...
The objective of this study was to examine the correlation between gene expression and lead (Pb) levels in blood children with autism (AU, n = 37) compared typically developing controls (TD, 15). We postulated that, though did not differ groups, AU might metabolize differently TD children. RNA isolated from processed on Affymetrix microarrays. Separate analyses covariance (ANCOVA) corrected for age gender were performed TD, AU, all subjects (AU + TD). To reduce false positives, only genes...
This study examined the effects of gender on RNA expression after ischemic stroke (IS). obtained from blood IS patients ( n = 51; 153 samples at ≤ 3, 5, and 24 hours) matched controls 52) were processed Affymetrix microarrays. Analyses covariance for versus control performed separately both genders regulated genes females compared with males. In all, 242, 227, 338 male-specific hours IS, respectively, which 59 all time points. Overall, 774, 3,437, 571 female-specific hours, 152 Male-specific...
Determining which small deep infarcts (SDIs) are of lacunar, arterial, or cardioembolic etiology is challenging, but important in delivering optimal stroke prevention therapy. We sought to distinguish lacunar from nonlacunar causes SDIs using a gene expression profile.A total 184 ischemic strokes were analyzed. Lacunar was defined as syndrome with infarction <15mm region supplied by penetrating arteries. RNA blood processed on whole genome microarrays. Genes differentially expressed between...
Since RNA expression differences have been reported in autism spectrum disorder (ASD) for blood and brain, differential alternative splicing (DAS) has ASD brains, we determined if there was DAS mRNA of subjects compared to typically developing (TD) controls, as well subgroups related cerebral volume.RNA from processed on whole genome exon arrays 2-4-year-old TD boys. An ANCOVA with age batch covariates used predict ALL (n=30), normal total volumes (NTCV), large (LTCV) controls (n=20).A 53...
Gene expression in blood was correlated with mercury levels of 2- to 5-year-old boys autism (AU) compared age-matched typically developing (TD) control boys. This done address the possibility that two groups might metabolize toxicants, such as mercury, differently. RNA isolated from and gene assessed on whole genome Affymetrix Human U133 microarrays. Mercury were measured using an inductively coupled plasma mass spectrometer. Analysis covariance (ANCOVA) performed partial correlations...
Differences in ischemic stroke between men and women have been mainly attributed to hormonal effects. However, sex differences immune response ischemia may exist. We hypothesized that differential expression of X-chromosome genes blood cells contribute with stroke.RNA levels 683 were measured on Affymetrix U133 Plus2.0 microarrays. Blood samples from patients obtained at ≤ 3 hours, 5 24 hours (n=61; 183 samples) after onset compared control subjects without symptomatic vascular diseases...
Abstract Aims Cerebral ischemia can lead to anxiety and cognitive impairment due the loss of hippocampal neurons. Facilitation endogenous neurogenesis in hippocampus is a potential therapeutic strategy for alleviating ischemia‐induced impairment. Progranulin (PGRN), secretory glycoprotein, has been reported have mitogentic effect on many cell types. However, it not clear whether PGRN enhances promotes functional recovery. Methods Adult male C57BL/6 mice were subjected permanent middle...
White matter hyperintensities (WMH) are areas of high signal detected by T2 and fluid-attenuated inversion recovery sequences on brain MRI. Although associated with aging, cerebrovascular risk factors, cognitive impairment, the pathogenesis WMH remains unclear. Thus, RNA expression was assessed in blood individuals without extensive to search for evidence oxidative stress, inflammation, other abnormalities described lesions brain.Subjects included 20 (WMH+), 45% whom had Alzheimer disease,...
Stressful life events induce abnormalities in emotional and cognitive behaviour. The endogenous opioid system plays an essential role stress adaptation coping strategies. In particular, the µ-opioid receptor (μR), one of major receptors, strongly influences memory processing that alterations μR signalling are associated with various neuropsychiatric disorders. However, it remains unclear whether contributes to impairments induced by acute stress. Here, we utilized pharmacological methods...
Background and Purpose— Deciphering whether a transient neurological event (TNE) is of ischemic or nonischemic etiology can be challenging. Ischemia cerebral tissue elicits an immune response in stroke attack (TIA). This response, as detected by RNA expressed cells, could potentially distinguish from TNE. Methods— Analysis 208 TIAs, strokes, controls, TNE was performed. blood processed on microarrays. TIAs (n=26) strokes (n=94) were compared with controls (n=44) to identify differentially...