A5014709286- Microfluidic and Bio-sensing Technologies
- Congenital Heart Disease Studies
- Microfluidic and Capillary Electrophoresis Applications
- Cardiac Structural Anomalies and Repair
- Cardiovascular and Diving-Related Complications
- Electrowetting and Microfluidic Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- 3D Printing in Biomedical Research
- Infective Endocarditis Diagnosis and Management
- Aortic Disease and Treatment Approaches
- Single-cell and spatial transcriptomics
- Circular RNAs in diseases
- Cardiac Valve Diseases and Treatments
- MicroRNA in disease regulation
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer, Hypoxia, and Metabolism
- Advanced Measurement and Detection Methods
- Photoacoustic and Ultrasonic Imaging
- Inertial Sensor and Navigation
- Neurological Disease Mechanisms and Treatments
- ATP Synthase and ATPases Research
- Neuroscience and Neural Engineering
- Atrial Fibrillation Management and Outcomes
- Digital Holography and Microscopy
Tsinghua University
2018-2024
The First Affiliated Hospital, Sun Yat-sen University
2024
Zhongshan Hospital
2024
Fudan University
2024
Yili Normal University
2024
Sun Yat-sen University
2024
Gannan Normal University
2022-2023
Henan Provincial People's Hospital
2022
Henan University
2022
Zhengzhou University
2022
Abstract Dielectrophoresis (DEP) particle separation has label‐free, well‐controllable, and low‐damage merits. Sidewall microelectrodes made of liquid metal alloy (LMA) inherits the additional advantage thick electrodes to generate impactful DEP force. However, existing LMA electrode‐based devices lack ability integrate large‐array in a compact footprint, severely limiting flow rate thus throughput. Herein, facile versatile method is proposed high‐density microfluidic devices, taking passive...
Single-cell impedance measurement is a label-free, noninvasive method for characterizing the electrical properties of single cells. At present, though widely used measurement, electric flow cytometry (IFC) and spectroscopy (EIS) are alone most microfluidic chips. In this paper, we present device combining IFC EIS techniques single-cell property measurement. The uses hydrodynamic constriction to passively trap cells coplanar electrodes obtain spectrum trapped cell via discrete data points...
Recent studies have suggested that exercise may be beneficial for delaying or attenuating Alzheimer's disease (AD). However, the underlying mechanisms were not clear. Microglia-mediated neuroinflammation is to play an important role in pathology of AD. The present study investigated effects treadmill on amyloid-β (Aβ) deposition and cognitive function amyloid precursor protein (APP)/PS1 mice early stage AD progression microglia-mediated was mainly analyzed. results demonstrated 12 weeks...
Abstract Cells have different intrinsic markers such as mechanical and electrical properties, which may be used specific characteristics. Here, we present a microfluidic chip configured with two opposing optical fibers four 3D electrodes for multiphysical parameter measurement. The leverages to capture stretch single cell uses achieve rotation of the cell. According stretching deformation spectrum, dielectric properties can extracted. We provided proof concept by testing five types cells...
Mechanical properties of single cells are important label-free biomarkers normally measured by expensive and complex imaging systems. To unlock this limit allow mechanical comparable across different measurement platforms, camera-free intrinsic cytometry (CFIMC) is proposed for on-the-fly two major parameters, that is, Young's modulus E fluidity β, cells. CFIMC adopts a framework couples the impedance electrodes with constriction channel spatially, so signals contain dynamic deformability...
Non-invasive and rapid imaging technique at subcellular resolution is significantly important for multiple biological applications such as cell fate study. Label-free refractive-index (RI)-based 3D tomographic constitutes an excellent candidate of cellular structures, but its full potential in long-term spatiotemporal observation locked due to the lack efficient integrated system. Here, a RI system incorporating cutting-edge white light diffraction phase microscopy module with stability,...
A passive strategy for deterministic single-cell trapping and release.
Electrical properties of single cells are important label-free biomarkers disease and immunity. At present, impedance flow cytometry (IFC) provides means for high throughput characterization single-cell electrical properties. However, the accuracy spherical single-shell model widely used in IFC has not been well evaluated due to lack reliable reproducible particles with true-value parameters as benchmarks. Herein, a method is proposed evaluate cell-sized unilamellar liposomes synthesized...
As a label-free and high-throughput single cell analysis platform, impedance flow cytometry (IFC) suffers from clogging caused by narrow microchannel as mechanical constriction (MC). Current sheath (SC) solutions lack systematic evaluation of the performance proper guidelines for fluid. Herein, we hypothesize that viscosity non-conductive liquid is key to SC, propose employ viscous in SC unlock tradeoff between sensitivity throughput, while ensuring measurement accuracy. By placing MC series...
Recent study has demonstrated that high-intensity interval training (HIIT) and moderate-intensity continuous (MICT) have the same effect to alleviate β-amyloid pathology in hippocampus of APPswe/PS1dE9 (APP/PS1) mice. Activation nucleotide binding oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3) inflammasome is pivotal been accelerate accumulation. The present aimed examine whether exercise-induced reduction was associated with changes NLRP3 activation. APP/PS1...
In this study, we proposed a microfluidic device with compact structures integrating multiple modalities for cell capture, pairing, fusion, and culture. The is composed of upper lower parts. part configured electrodes capture wells used trapping/pairing/fusion, while the corresponding culture Dielectrophoresis to enable accurate trapping pairing in wells. Moreover, paired cells are fused flexibly by either electrical pulses or polyethylene glycol (PEG) buffer. then transferred on-chip simply...
The three-dimensional (3D) rotation of a single cell is fundamental manipulation process at the cellular level for physiological and pathological characterization. However, motion cells affected by factors such as fluid forces gravitation forces; hence, it difficult to maintain stable spatial position cell. Therefore, ensuring positions single-cell an essential problem that needs be addressed. In this paper, we present microfluidic chip based on dielectrophoresis, self-adaptive localization...
High content screening (HCS) plays an important role in biological applications and drug development. Existing techniques fail to simultaneously meet multiple needs of throughput, efficiency sample chemical consumption, real-time imaging a large view at high resolution. Leveraging advances microfluidics technology, we setup new paradigm large-scale, high-content solutions for rapid processes, like cardiotoxicity. The designed microfluidic chips enable 10 types drugs each with 5...
Exercise plays a beneficial role in the management of Alzheimer's disease (AD), but its effects on brain metabolism are still far from being understood. Here, we examined behavioral changes APP/PS1 mice after high-intensity interval training (HIIT) and moderate-intensity continuous (MICT) analyzed metabolomics profiles hippocampus, cortex, hypothalamus by using nuclear magnetic resonance spectroscopy to explore potential metabolic mechanisms. The results demonstrate that both HIIT MICT...
Mulching is a widely used agricultural water conservation measure in the semiarid regions of Northwest China. In order to explore response process different film mulching methods soil microorganisms, we characterized effect on microbial diversity and community structure characteristics root zone drip-irrigated maize during heading maturity stages using high-throughput sequencing 16SrDNA ITS amplicons combined with bioinformatics analysis. Full (FM) was contrasted controls no (NM)...
Imaging flow cytometry allows image-activated cell sorting (IACS) with enhanced feature dimensions in cellular morphology, structure, and composition. However, existing IACS frameworks suffer from the challenges of 3D information loss processing latency dilemma real-time operation. Herein, we establish a neuromorphic-enabled video-activated sorter (NEVACS) framework, designed to achieve high-dimensional spatiotemporal characterization content alongside high-throughput particles wide field...
Increasing studies have suggested that microRNAs (miRNAs) are involved in the development of gliomas. MicroRNA-216a has been reported to be a tumor-associated miRNA many types cancer, either as an oncogene or tumor suppressor. However, little is known about function miR-216a The present study was designed explore potential role We found significantly decreased glioma tissues and cell lines. Overexpression suppressed proliferation, migration, invasion cells. Leucine-rich repeat-containing G...