A5016732331- Oral microbiology and periodontitis research
- Oral Health Pathology and Treatment
- Oral and gingival health research
- HIV/AIDS oral health manifestations
- Salivary Gland Disorders and Functions
- Periodontal Regeneration and Treatments
- Epigenetics and DNA Methylation
- Immune Response and Inflammation
- Diabetes and associated disorders
- Bone health and treatments
- Bone Metabolism and Diseases
- dental development and anomalies
- Inflammatory mediators and NSAID effects
- Pediatric health and respiratory diseases
- Dental Radiography and Imaging
- Oral and Maxillofacial Pathology
- Antimicrobial Peptides and Activities
- Antifungal resistance and susceptibility
- Dental Health and Care Utilization
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Genetic Syndromes and Imprinting
- Preterm Birth and Chorioamnionitis
- Asthma and respiratory diseases
- Neonatal and Maternal Infections
- Laser Applications in Dentistry and Medicine
University of North Carolina at Chapel Hill
2014-2023
University of North Carolina System
2005-2020
British Society of Periodontology
2006-2016
University of North Carolina Health Care
2008-2016
Universidade Estadual de Campinas (UNICAMP)
2000-2015
Communities In Schools of Orange County
2014
David H. Murdock Research Institute
2009
Duke Medical Center
2007
Methodist University of Piracicaba
2005
University of Washington
2003-2004
A molecular epidemiologic study provided the opportunity to characterize biology of biofilm-gingival interface (BGI) in 6,768 community-dwelling subjects.Disease classifications and multivariable models were developed using clinical, microbial, inflammatory, host-response data. The purpose was identify new clinical categories that represented distinct biologic phenotypes based upon DNA checkerboard analyses eight plaque bacteria, serum immunoglobulin G (IgG) titers 17 gingival crevicular...
Background: Few studies have examined the potential effects of periodontal treatment during pregnancy on outcomes, status, and inflammatory biomarkers. Methods: A randomized, delayed‐treatment, controlled pilot trial was conducted to evaluate second‐trimester scaling root planing use a sonic toothbrush rate preterm delivery (<37 weeks gestation). Secondary outcome measures included changes in levels eight oral pathogens, gingival crevicular fluid (GCF) interleukin‐1β (IL‐1β),...
The aim of this pilot investigation was to determine if microRNA expression differed in the presence or absence obesity, comparing gingival biopsies obtained from patients with without periodontal disease. Total RNA extracted biopsy samples collected 20 patients: 10 non-obese (BMI < 30 kg/m 2 ) and obese > ), each group 5 periodontally healthy sites chronic periodontitis sites. MicroRNA patterns were assessed a quantitative PCR array survey 88 candidate species. Four databases used...
Pathological shifts of the human microbiome are characteristic many diseases, including chronic periodontitis. To date, there is limited evidence on host genetic risk loci associated with periodontal pathogen colonization. We conducted a genome-wide association (GWA) study among 1,020 white participants Atherosclerosis Risk in Communities Study, whose diagnosis ranged from healthy to severe periodontitis, and for whom “checkerboard” DNA-DNA hybridization quantification 8 pathogens was...
Genome-wide association studies (GWAS) of chronic periodontitis (CP) defined by clinical criteria alone have had modest success to-date. Here, we refine the CP phenotype supplementing data with biological intermediates microbial burden (levels eight periodontal pathogens) and local inflammatory response (gingival crevicular fluid IL-1β) derive complex traits (PCTs) via principal component analysis. PCTs were carried forward to GWAS (∼2.5 million markers) identify PCT-associated loci among...
Maternal oral infection, caused by bacteria such as C. rectus or P. gingivalis, has been implicated a potential source of placental and fetal infection inflammatory challenge, which increases the relative risk for pre-term delivery growth restriction. Intra-uterine restriction also reported in various animal models infected with organisms. Analyzing tissues growth-restricted mice, we found down-regulation imprinted Igf2 gene. Epigenetic modification genes via changes DNA methylation plays...
The goal of this study is to characterize the changes in 33 biomarkers within gingival crevicular fluid during 3-week induction and 4-week resolution stent-induced, biofilm overgrowth mediated, experimental gingivitis humans.Experimental was induced 25 subjects for 21 days followed by treatment with a sonic powered toothbrush 28 days. Clinical indices fluids were collected weekly biweekly resolution. Samples analysed using bead-based multiplexing analysis simultaneous measurements each...
Levels of prostaglandin E 2 and the prostaglandin-endoperoxide synthase-2 ( PTGS2, or COX-2) increase in actively progressing periodontal lesions, but decrease chronic disease. We hypothesized that inflammation is associated with altered DNA methylation levels within PTGS2 promoter, effects on COX-2 mRNA expression. promoter from periodontally inflamed gingival biopsies showed a 5.06-fold as compared non-inflamed samples (p = 0.03), odds CpG site group 4.46 times higher than same 0.016). The...
Background: Tumor necrosis factor‐α (TNF‐α) plays a central role in the molecular pathogenesis of periodontal disease. However, epigenetic regulation attributable to microbial and inflammatory signals at biofilm–gingival interface are poorly understood. In this study, DNA methylation alteration within TNFA promoter human gingival biopsies from different stages disease is investigated regulatory mechanism transcription by explored. Methods: Gingival were obtained 17 patients with chronic...
Multifactorial etiological factors contribute to denture stomatitis (DS), a type of oral candidiasis; however, unlike other candidiasis, DS can occur in healthy person wearing denture. In this study, we therefore attempt explore the association between candida, denture, and mucosal tissue using (1) exfoliative cytology, (2) candidal levels present saliva, on tissues surfaces, (3) salivary flow rate xerostomic symptoms.A cross-sectional study enrolled 32 edentulous participants, 17 without as...
The goal of this investigation was to determine whether epigenetic modifications in the IFNG promoter are associated with an increase transcription different stages periodontal diseases.DNA extracted from gingival biopsy samples collected 47 total sites subjects: 23 periodontally healthy sites, 12 experimentally induced gingivitis and chronic periodontitis sites. Levels DNA methylation within containing six CpG dinucleotides were determined using pyrosequencing technology. Interferon gamma...
Abstract There is no agnostic GWAS evidence for the genetic control of IL-1β expression in periodontal disease. Here we report a “high” gingival crevicular fluid among 4910 European-American adults and identify association signals IL37 locus. rs3811046 at this locus ( p = 3.3 × 10 −22 ) associated with severe chronic periodontitis (OR 1.50; 95% CI 1.12–2.00), 10-year incident tooth loss (≥3 teeth: RR 1.33; 1.09–1.62) aggressive 1.12; 1.01–1.26) an independent sample 4927 German/Dutch adults....
Background: Genetic polymorphisms in the vitamin D receptor (VDR) gene are associated with bone homeostasis and diseases which loss is a cardinal sign. The aim of this study was to determine whether chronic periodontal disease Brazilian population VDR gene. Methods: Clinical examination recordings probing depth clinical attachment level were performed 113 unrelated adults who divided into two groups: 44 healthy individuals (control group) 69 subjects periodontitis (CP). DNA obtained from...
Parathyroid hormone (PTH) functions as a major mediator of bone remodeling and an essential regulator calcium homeostasis. In addition to the well-established catabolic effects (activation resorption) PTH, it is now recognized that intermittent PTH administration has anabolic (promotion formation). The aim this study was investigate whether in rodents would block alveolar loss observed rats when ligature model periodontitis used. Morphometric analysis showed (40 μg/kg) able protect tooth...
<i>Background/Aims:</i> Increasing evidence suggests that clinical signs of periodontal disease are independently associated with renal impairment. However, no studies have examined the possible linkage kidney serum antibody to oral pathogens. <i>Methods:</i> The status was assessed in an older community-dwelling population (Dental Atherosclerosis Risk Communities) include: measurements; biofilm microbial composition by DNA checkerboard, and immunoglobulin-γ (IgG)...
Background: To our knowledge, changes in the patterns of whole‐transcriptome gene expression that occur during induction and resolution experimental gingivitis humans were not previously explored using bioinformatic tools. Methods: Gingival biopsy samples collected from 14 subjects a 28‐day stent‐induced model, followed by treatment, at days 28 through 35 analyzed gene‐expression arrays. Biopsy different sites within each subject baseline (day 0), peak 28), 35) processed Gene‐expression data...
Background In COPD patients, fatal and non-fatal respiratory-related events are influenced by age, severity of respiratory disease, comorbidities. Objectives Analyze the effects edentulism, periodontal disease systemic biomarkers inflammation on occurrence serious among subjects with COPD. Methods Cases were identified from Dental Atherosclerosis Risk in Communities study. Edentulism was defined as study participants without any natural teeth or implants. Participants one more (comprising...
1. Troglitazonewas the first thiazolidinedione approved for clinical use in treatment of non-insulin-dependent diabetes mellitus. During investigations drug-drug interactions with therapeutics (terfenadine and cyclosporine) known to be metabolized by CYP3A4, pharmacokinetic were noted upon troglitazone multiple-dose treatments. The nature suggested induction CYP3A enzymes. 2. Primary cultures human hepatocytes used investigate potential respect CYP2B6 CYP1A1/2. In hepatocytes, induced both...