A5060431070- Immune Cell Function and Interaction
- Viral-associated cancers and disorders
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Growth Hormone and Insulin-like Growth Factors
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Lymphoma Diagnosis and Treatment
- Herpesvirus Infections and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Cervical Cancer and HPV Research
- Glycosylation and Glycoproteins Research
- Acute Myeloid Leukemia Research
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Hematopoietic Stem Cell Transplantation
- Lung Cancer Diagnosis and Treatment
- T-cell and B-cell Immunology
- Legume Nitrogen Fixing Symbiosis
- Cassava research and cyanide
- Chemical Synthesis and Analysis
- Cell death mechanisms and regulation
- interferon and immune responses
- HIV Research and Treatment
University of Birmingham
2008-2021
Institute of Immunology
2020
Immune Regulation (United Kingdom)
2013
Cancer Research UK Clinical Trials Unit
2008-2011
Cancer Research UK
2007
Plant & Food Research
1994-2004
Slagelse Hospital
2004
Peter MacCallum Cancer Centre
1998
University of Groningen
1997
Memorial Hospital
1973
Virus-specific CD4+ T cells are key orchestrators of host responses to viral infection yet, compared with their CD8+ cell counterparts, remain poorly characterized at the single level. Here we use nine MHC II–epitope peptide tetramers visualize human Epstein–Barr virus (EBV), causative agent infectious mononucleosis (IM), a disease associated large virus-specific responses. We find that, while not approaching expansions in magnitude, activated specific for epitopes latent antigen EBNA2 and...
Intratumoral hypoxia and immunity have been correlated with patient outcome in various tumor settings. However, these factors are not currently considered for treatment selection head neck cancer (HNC) due to lack of validated biomarkers. Here we sought develop a hypoxia-immune classifier potential application prognostication prediction response targeted therapy.
CD4+ T cells are essential for immune protection against viruses, yet their multiple roles remain ill-defined at the single-cell level in humans. Using HLA class II tetramers, we studied functional properties and clonotypic architecture of EBV-specific patients with infectious mononucleosis, a symptomatic manifestation primary EBV infection, long-term healthy carriers EBV. We found that infection elicited oligoclonal expansions TH1-like armed cytotoxic proteins responded immediately ex vivo...
Within the basolateral membrane of thyroid follicular epithelial cells, two transporter proteins are central to hormone (TH) biosynthesis and secretion. The sodium iodide symporter (NIS) delivers from bloodstream into thyroid, after TH biosynthesis, monocarboxylate 8 (MCT8) mediates secretion gland. Pituitary tumor-transforming gene-binding factor (PBF; PTTG1IP) is a protooncogene that up-regulated in cancer binds NIS modulates its subcellular localization function. We now show PBF MCT8...
CMV infection is a significant cause of morbidity and mortality in immunocompromised individuals, the development vaccine high priority. Glycoprotein B (gB) leading candidate but glycoprotein H (gH) pentameric complex now recognized as major target for neutralizing Abs. However, little known about T cell immune response against gH L (gL) this likely to be an important attribute immunogenicity. In study, we examine contrast magnitude phenotype gB, gH, gL within healthy donors. gB-specific...
Despite highly toxic treatments, head and neck squamous cell carcinoma (HNSCC) have poor outcomes. There is an unmet need for more effective, less therapies. Repurposing of clinically-approved drugs, with known safety profiles, may provide a time- cost-effective approach to address this need. We developed the AcceleraTED platform repurpose drugs HNSCC treatment; using in vitro assays (cell viability, clonogenic survival, apoptosis) vivo models (xenograft tumors NOD/SCID/gamma mice)....
Epstein Barr Virus (EBV) infects more than 95% of the population whereupon it establishes a latent infection B-cells that persists for life under immune control. Primary EBV can cause infectious mononucleosis (IM) and long-term viral carriage is associated with several malignancies certain autoimmune diseases. Current efforts developing prophylactic vaccination have focussed on neutralising antibodies. An alternative strategy, could enhance efficacy such vaccines or be used alone, to...
ABSTRACT We have evaluated a neutralizing antibody assay which uses human papillomavirus (HPV) type 16 (HPV-16) and HPV-18 pseudovirions carrying secretory alkaline phosphatase reporter gene can potentially measure functionally relevant HPV type-specific antibodies. The reproducibility of the was excellent; for HPV-16, intra- interassay kappa values were 0.95 0.90, respectively; HPV-18, corresponding 0.90 0.90. This used to describe kinetics response in cohort 42 young women who recruited...
Four generic heterobifunctional reagents, namely 2-(2-chloro-5-methyl-1,3,2-dioxaphosphorinan-5-yl)methoxyacetic acid methyl ester, p-sulfide, p-oxide, 2-(2-mercapto-5-methyl-1,3,2-dioxaphosphorinan-5-yl)methoxyacetic bispotassium salt, p-sulfide-, and (2-methoxy-5-methyl-1,3,2-dioxaphosphorinan-5-yl)methoxyacetic acid, have been synthesized used to prepare organophosphate, thiophosphate, dithiophosphate haptens containing a functional carboxyl group which can be conjugate the proteins....
2‐(2‐Mercapto‐5‐methyl‐1,3,2‐dioxaphosphorinan‐5‐yl,2‐sulphide)methoxyacetic acid has been synthesized and used to prepare an azinphos hapten protein conjugates. Monoclonal antibodies of high affinity against the pesticide azinphos‐methyl were prepared from mice immunized with hapten‐ovalbumin conjugate. The best monoclonal antibody could be in indirect enzyme‐linked immunosorbent assay (ELISA) quantify at concentrations 50 pg ml‐1 2 ng (2.5–100 pg/assay). corresponding limit detection was...
Abstract Coronatine (COR) is composed of two structural components, coronafacic acid (CFA) and the amino coronamic (CMA), linked by an amide bond. Monoclonal antibodies (MAbs) were prepared against COR incorporated into competitive ELISAs. MAbs secreted hybridoma cells which had been from mice immunized with conjugated, through free carboxyl group on CMA, to ovalbumin, available groups increased derivatization propyl diamine via groups. coronafacoyl valine could be quantified at 6.800 ng...
Epstein-Barr virus (EBV)-associated post-transplant lymphoproliferative disease (PTLD) is a life-threatening complication of T-lymphocyte deplete allogeneic hematopoietic stem cell transplantation (allo-HSCT). For patients with PTLD refractory to Rituximab, donor lymphocyte infusion (DLI) established as successful option for salvage therapy. However, although in vivo expansion has been correlated good clinical outcome following DLI, the specificity and functional characteristics EBV-specific...
Six hybridoma clones were obtained that secreted monoclonal antibodies against the aspartate aminotransferase-P1 (AAT-P1) isoenzyme from root nodules of Lupinus angustifolius [L.] cv Uniharvest. This enzyme is found constitutively in plant cytosol fraction. The produced all immunoglobulin G1 class, recognized two distinct epitopes on protein, and represented major paratopes fraction sera taken mice rabbits immunized with pure AAT-P1 protein. One these was unique to lupin nodule AAT-P1. other...
Chitin hexamer was conjugated to mono-BOC-diaminoethane, n -aminobutyric acid or glycine using reductive amination. Each hapten ovalbumin (OVA) mouse serum albumin (MSA) for immunization and immunoassay, respectively. Serum from a immunized with the diaminoethane conjugate, demonstrated competition chitins in modified indirect immunoassay.
A trans-acting protein interacting with a specific sequence motif proximal to the transcriptional start site of L-asparaginase promoter has been observed previously (E. Vincze, J. M. Reeves, E. Lamping, K. F. Farnden, and P. H. S. Reynolds, Plant Mol. Biol. 26:303-311, 1994). Gel retardation experiments in which extracts Mesorhizobium loti developing nodules were used suggested bacterial origin for repressor binding (rep2037). Nodulation tests performed by using different Fix(-) Tn5 mutants...