A5039597729- Pregnancy and preeclampsia studies
- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- COVID-19 Clinical Research Studies
- COVID-19 Impact on Reproduction
- Sepsis Diagnosis and Treatment
- Atrial Fibrillation Management and Outcomes
- Long-Term Effects of COVID-19
- Birth, Development, and Health
- Dialysis and Renal Disease Management
- IL-33, ST2, and ILC Pathways
- Reproductive System and Pregnancy
- Inflammasome and immune disorders
- Extracellular vesicles in disease
- Cardiovascular Issues in Pregnancy
- Blood properties and coagulation
- Blood Coagulation and Thrombosis Mechanisms
- Acute Myeloid Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Histone Deacetylase Inhibitors Research
- COVID-19 and healthcare impacts
- Mechanical Circulatory Support Devices
- Liver Disease Diagnosis and Treatment
- T-cell and B-cell Immunology
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2018-2024
Universitat de Barcelona
2016-2024
Hospital Clínic de Barcelona
2017-2024
Josep Carreras Leukaemia Research Institute
2021
Institut d'Investigacions Biomèdiques de Barcelona
2018
Atypical hemolytic uremic syndrome is a form of thrombotic microangiopathy caused by dysregulation the alternative complement pathway. There evidence showing activation in other microangiopathies. The aim this study was to evaluate different microangiopathies and monitor treatment response.Complement assessed exposing endothelial cells sera or activated-patient plasma-citrated plasma mixed with control pool (1:1)-to analyze C5b-9 deposits immunofluorescence. Patients atypical (n=34) at...
Abstract There is a link between depression, cardiovascular events and inflammation. We have explored this connection through endothelial dysfunction, using in vivo vitro approaches. evaluated circulating biomarkers of dysfunction patients with major depression at their diagnosis (MD-0) during antidepressant treatment the selective serotonin reuptake inhibitor escitalopram, for 8 24 weeks (MD-8 MD-24). Results were always compared matched healthy controls (CON). measured cells (CECs)...
Preeclampsia is a pregnancy-specific multisystem disorder and leading cause of maternal perinatal morbidity mortality. The exact pathogenesis this multifactorial disease remains poorly defined. We applied proteomics analysis on blood samples collected from 14 singleton pregnancies with early-onset severe preeclampsia 6 uncomplicated to investigate the pathophysiological pathways involved in specific subgroup preeclampsia. Maternal was drawn at diagnosis for cases matched gestational age...
COVID-19 presents a spectrum of signs and symptoms in pregnant women that might resemble preeclampsia. Differentiation between severe preeclampsia is difficult some cases.To study biomarkers endothelial damage, coagulation, innate immune response, angiogenesis pregnancy addition to vitro alterations cells exposed sera from with COVID-19.Plasma samples were obtained infection classified into mild (n=10) or (n=9) normotensive pregnancies as controls patients (n=13). A panel plasmatic was...
Accelerated atherosclerosis in chronic kidney disease (CKD) is preceded by endothelial dysfunction (ED), which exhibits a proinflammatory and prothrombotic phenotype enhanced oxidative stress. In this study, the effect of several compounds with anti-inflammatory and/or antioxidant properties on uremia-induced has been evaluated an vitro model.Endothelial cells (ECs) were exposed to sera from uremic patients absence presence flavonoids apigenin, genistein quercetin, enzyme mimetics (AEM)...
Endotheliopathy is a key element in COVID-19 pathophysiology, contributing to both morbidity and mortality. Biomarkers distinguishing different phenotypes from sepsis syndrome remain poorly understood.To characterize circulating biomarkers of endothelial damage clinical disease stages compared with normal volunteers.Patients pneumonia (n = 49) were classified into moderate, severe, or critical (life-threatening) disease. Plasma samples collected within 48 72 h hospitalization analyze...
Angiogenesis and endothelial activation dysfunction have been associated with acute graft-versus-host disease (aGVHD), pointing to the endothelium as a potential target for pharmacological intervention. Defibrotide (DF) is drug an endothelium-protective effect that has approved treatment of veno-occlusive disease/sinusoidal obstruction syndrome after allogeneic hematopoietic cell transplantation. Clinical data suggest DF also reduces incidence aGVHD; however, mechanisms DF-mediated aGVHD...
Endothelial dysfunction is an earlier contributor to the development of atherosclerosis in chronic kidney disease (CKD), which role epigenetic triggers cannot be ruled out. protective strategies, such as defibrotide (DF), may useful this scenario. We evaluated changes induced by CKD on endothelial cell proteome and explored effect DF mechanisms involved. Human umbilical cord vein cells were exposed sera from healthy donors (n = 20) patients with end-stage renal haemodialysis 20)....
Abstract Graft‐versus‐host disease (GVHD) is a complication of allogeneic haematopoietic cell transplantation. Endothelial injury crucial as pathophysiological substrate for GVHD. GVHD first‐line treatment high‐dose corticosteroids, although some patients are steroid‐refractory. Through the present study, we compared endothelial proteomic profiles in response to serum from steroid‐refractory acute (SR‐aGVHD) and steroid‐sensitive (SS‐aGVHD) patients. Blood samples SR‐aGVHD ( n = 4) SS‐aGVHD...
Current laboratory tests fail to evaluate the hemostatic function of platelets in patients with thrombocytopenia. We investigated use Total Thrombus-Formation Analysis System (T-TAS
Stent thrombosis (ST) is a catastrophic event and efforts to reduce its incidence by altering blood-stent interactions are longstanding. A new electret coating technology that produces long-lasting negative charge on stent surface could make them intrinsically resistant thrombosis. We assessed the thrombogenicity of stents using an annular perfusion model with confocal microscopy, determined efficacy confer thrombo-resistant properties standard stents. Using chamber, Bare Metal (BMS),...
Background Association between global platelet function and the risk of venous thromboembolic disease (VTE) has been proposed, though mechanisms do not involve increased aggregation. However, adhesiveness systematically explored in VTE patients. Objectives To evaluate adhesive functions Methods Platelet adhesion was evaluated by using whole blood samples from patients, selected based on short closure times PFA-100 (n = 54), matched healthy individuals 57) in: (i) PFA-100, (ii) a cone plate...
Preeclampsia (PE) is a pregnancy complication characterized by hypertension, proteinuria, endothelial dysfunction, and complement dysregulation. Placenta-derived extracellular vesicles (EVs), necessary in maternal-fetal communication, might contribute to PE pathogenesis. Moreover, neutrophil traps (NETs) play pathogenic role other complement-mediated pathologies, their contribution remains unexplored.
Identification of qualitative variants von Willebrand disease (VWD) can be a diagnostic challenge because discrepant results obtained in the multiple laboratory tests available for its appropriate classification. We report two cases infrequent inherited VWD with unclear preliminary test panel at time first consultation and that were finally diagnosed as type 2A/IID c.8318 G > C, p.Cys2773Ser mutation 2M c.4225 T G, p.Val1409Phe mutation, respectively. The description these highlights despite...
Introduction: Extracellular vesicles (EVs) are membrane structures secreted by cells that have been described as potential mediators of pathogenic mechanisms in endothelial dysfunction (ED), causing stimulation or suppression gene expression. The ED patients with End stage kidney disease (ESKD) and type 1 diabetes mellitus (T1DM) not well defined, although EVs may play an important role. In the present study, we report on relevance outcome T1DM ESKD before after one year a simultaneous...