A5030824768- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Cellular transport and secretion
- ATP Synthase and ATPases Research
- RNA Interference and Gene Delivery
- Animal Genetics and Reproduction
- Nuclear Structure and Function
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Gastroesophageal reflux and treatments
- Parasitic Infections and Diagnostics
- Epigenetics and DNA Methylation
- Trypanosoma species research and implications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA regulation and disease
- Parkinson's Disease Mechanisms and Treatments
- Neurobiology and Insect Physiology Research
- Neuroscience and Neural Engineering
- Metabolism and Genetic Disorders
- Endoplasmic Reticulum Stress and Disease
- MicroRNA in disease regulation
- Polymer Surface Interaction Studies
- Microtubule and mitosis dynamics
- Hippo pathway signaling and YAP/TAZ
- Transgenic Plants and Applications
Universidad Católica del Norte
2023-2024
Pontificia Universidad Católica de Chile
2018-2023
University of Antofagasta
2018-2023
Autosomal Dominant Optic Atrophy (ADOA), a disease that causes blindness and other neurological disorders, is linked to
Inner mitochondrial membrane fusion and cristae shape depend on optic atrophy protein 1, OPA1. Mutations in OPA1 lead to autosomal dominant (ADOA), an important cause of inherited blindness. The Guanosin Triphosphatase (GTPase) GTPase effector domains (GEDs) are essential for fusion; yet, their specific roles remain elusive. Intriguingly, patients carrying mutations have a higher risk developing more severe multisystemic symptoms addition atrophy, suggesting pathogenic contributions the GED...
The Wnt signaling pathway induces various responses underlying the development and maturation of nervous system. ligands are highly hydrophobic proteins that limit their diffusion through an aqueous extracellular medium to a target cell. Nevertheless, attachment small vesicles-like exosomes is one described mechanisms allow transport under this condition. Some in these vehicles expected be dependent on post-translational modifications such as acylation. determining loading delivery cells...
The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system has been widely used in animals as an efficient genome editing tool. In fish cells, the technique difficult to implement due lack of proper vectors that use active promoters drive expression both small guide RNA (sgRNA) and
In this work, samarium-doped BaTiO
Parkinson’s disease (PD) is a neurodegenerative characterized by motor symptoms and dopaminergic cell loss. A pre-symptomatic phase non-motor precedes the onset of alterations. Two recent PET studies in human carriers mutations associated with familial PD demonstrate an early serotonergic commitment—alteration SERT binding—before any or dysfunction, that is, at putative stages. These findings support hypothesis alterations system could contribute to progression PD, idea difficult be tested...
Neurons release neurotransmitters at a specialized region of the presynaptic membrane, active zone (AZ), where complex meshwork proteins organizes apparatus. The formation this proteinaceous cytomatrix AZ (CAZ) depends on precise homo- and hetero-oligomerizations distinct CAZ proteins. protein CAST1/ERC2 contains four coiled-coil (CC) domains that interact with other proteins, but also promote self-assembly, which is an essential step for its integration during formation. self-assembly...
Nuclear processes such as transcription and RNA maturation can be impacted by subnuclear compartmentalization in condensates nuclear bodies. Here, we characterize the nature of granules formed REST corepressor 2 (RCOR2), a protein essential for pluripotency maintenance central nervous system development. Using biochemical approaches high-resolution microscopy, reveal that RCOR2 is localized speckles across multiple cell types, including neurons brain. forms complexes with speckle components...
Cells of vertebrate and invertebrate organisms express proteins specialized in membrane channel–based cell–cell communication that are absent unicellular organisms. We recently described the prediction some members large-pore channel family kinetoplastids, consisting called unnexins, which share several structural features with innexin pannexin proteins. Here, we demonstrated unnexin1 protein (Unx1) is delivered to cell membrane, displaying a topology four transmembrane domains C N termini...
Regulated systems for transgene expression are useful tools in basic research and a promising platform biomedicine due to their regulated by an inducer. The emergence of optogenetics enabled the construction light-switchable systems, enhancing spatial temporal resolution transgene. LightOn system is optogenetic tool that regulates gene interest using blue light as This based on photosensitive protein (GAVPO), which dimerizes binds UASG sequence response light, triggering downstream...
Abstract Lysine-specific demethylase 1A (LSD1) binds to the REST corepressor (RCOR) protein family of corepressors erase transcriptionally active marks on histones. Functional diversity in these complexes depends type RCOR included, which modulates catalytic activity complex. Here, we studied duplicative history and LSD gene families analyzed evolution their interaction. We found that genes are product two rounds whole-genome duplications occurred early vertebrate evolution. In contrast,...
Molecular brain therapies require the development of molecular switches to control gene expression in a limited and regulated manner time space. Light-switchable systems allow precise with an enhanced spatio-temporal resolution compared chemical inducers. In this work, we adapted existing light-switchable Light-On system into lentiviral platform, which consists two modules: (i) one for blue trans-activator GAVPO (ii) second module containing inducible-UAS promoter (UAS) modulated by...
Abstract Background Nuclear processes such as transcription and RNA maturation can be impacted by subnuclear compartmentalization in condensates nuclear bodies. Here we characterize the nature of granules formed REST corepressor 2 (RCOR2), a protein essential for pluripotency maintenance central nervous system development. Results Using biochemical approaches high-resolution microscopy, reveal that RCOR2 is localized speckles across multiple cell types, including neurons brain. forms...