The simultaneous imaging and quantification of multiple intracellular microRNAs (miRNAs) are particularly desirable for the early diagnosis cancers. However, direct with absolute RNAs remains a great challenge, miRNAs, which have significantly different expression levels in living cells. We designed dual-signal switchable (DSS) nanoprobes using fluorescence-Raman signal switch. uptake dynamic behaviors probe monitored by its fluorescence signal. Meanwhile, real-time quantitative detection...

This study prepared a composite scaffold composed of curcumin and poly(ε‐caprolactone)‐poly(ethylene glycol)‐poly(ε‐caprolactone) (PCL‐PEG‐PCL, PCEC) copolymer using coelectrospinning technology. Incorporation into the polymeric matrix had an obvious effect on morphology dimension PCEC/curcumin fibers. The results in vitro anti‐oxidant tests cytotoxicity assay demonstrated that curcumin‐loaded PCEC fibrous mats significant efficacy low cytotoxicity. Curcumin could be sustainably released...

Background: MicroRNAs (miRNAs) play important roles in many biological processes, including cancer development.Among those miRNAs, miR-143 shows tumor-suppressive activity some human cancers.However, the function and mechanism of lung cells remains unknown.Here we explored role cancer.Results: According to qRT-PCR, found that was notably down-regulated 19 NSCLC tissues 5 cell lines.In vitro experiments showed us could significantly suppress migration invasion lines while it had no effects on...

Sensitive detection of microRNAs (miRNAs) that serve as a disease marker could advance the diagnosis and treatment diseases. Many methods used for quantitative miRNAs, such PCR-based approaches or hybridization chain reaction, have presented challenges due to complicated time-consuming-procedures are required. In this manuscript, simple triggerable mutually amplified signal (TMAS) probe was designed enriched within center microfluidic chip then one-step via surface enhanced Raman scattering...

For the first time, we report synthesis of Ag NPs arranged in a helical structure on chiral MOF. This material can serve as new type SERS sensor for efficient recognition enantiomers.

Simultaneous detection of cancer biomarkers holds great promise for the early diagnosis different cancers. However, in presence high-concentration biomarkers, signals lower-expression are overlapped. Existing techniques not suitable simultaneously detecting multiple at concentrations with significantly orders magnitude. Here, we propose an asymmetric signal amplification method levels. Using bifunctional probe, a linear mode responds to markers, and quadratic low-concentration markers. With...

MicroRNAs (miRNAs) play important roles in biological processes. Designing a sensitive, selective, and rapid method of miRNA detection is crucial for research. Here, with reciprocal signal amplification (RSA) probe, this work established novel surface-enhanced Raman scattering (SERS)-microfluidic approach the quantitative analysis miRNA. First, via DNAzyme self-assemble cycle reaction, two types SERS signals produce amplified changes. The sum absolute value first adopted miRNA, which results...

Abstract A new photoelectrochemical (PEC) biosensor was developed by using carboxyl‐functionalized graphene and CdSe nanoparticles. This sensitive interface then successfully applied to detection of thrombin based on the dual‐quenched effect PEC nanoparticle, which relied electron transfer a bipyridinium relay energy AuNPs. After recognition with an aptamer, nanoparticle removed signal‐on obtained. Moreover, bio‐barcode technique used in preparation could avoid cross‐reaction enhances...

In this work, an enzyme-free signal amplified detection platform is described for miRNA with a DNA fueled molecular machine. Coupling SERS technology multiple amplification modes, flexible biosensing system exhibits high sensitivity and specificity.

The detection of exosomes is critical for health monitoring and disease diagnosis. However, their small size low concentration present significant challenges. In this study, we designed a dual-control microchip integrated with surface-enhanced Raman scattering (SERS) signal amplification method. By employing separate chambers isolation detection, method achieves magnetic separation control DNA cascade electrokinetic enrichment detection. step captures isolates in magnetic-controlled reaction...

An ultrasensitive surface-enhanced Raman scattering (SERS) detection system based on DNA machine isothermal amplification is reported to detect a specific sequence for the first time and was successfully applied cancer cells.

Imaging and detecting microRNAs (miRNAs) is of central importance in tumor cell analysis. It stays challenging to establish simple, accurate, sensitive analytical assays for imaging detection miRNA a single living cell, because intracellular complex environment sequence similarity. Herein, we designed dual-signal twinkling probe (DSTP) with triplex-stem structure which employed fluorescence-SERS signal reciprocal switch. The spatiotemporal dynamics the molecular uptake are monitored by...

The expression of microRNAs (miRNAs) is critical in gene regulation and has been counted into disease diagnosis marks. Precise imaging quantification miRNAs could afford the important information for clinical diagnosis. Here, two smart binary star ratio (BSR) probes were designed constructed, miRNA triggered connection reciprocal changes dual signals living cells. This multifunctional probe integrates fluorescence surface enhanced Raman scattering (SERS) imaging, with enzyme-free numerator...

A novel surface-enhanced Raman scattering (SERS) detection system is developed for proteins and nucleic acids based on a triple-helix molecular switch multiple cycle signal amplification, achieving high sensitivity, universality, rapid analysis, selectivity.

Radical surgery for colorectal cancer, associated with moderate to severe postoperative pain, needs multimodal analgesia opioid analgesia. Despite considerable advancements, the psychological implications and other side effects remain substantially unresolved. This study aimed investigate impact on mood, relative opioid, recovery of patients hydromorphone or sufentanil intravenous patient-controlled (IV-PCA) in a perioperative regimen undergoing radical cancer. Eighty elective laparoscopic...

A simple bifunctional surface-enhanced Raman scattering (SERS) assay based on primer self-generation strand-displacement polymerization (PS-SDP) is developed to detect small molecules or proteins in parallel. Triphosphate (ATP) and lysozyme are used as the models of proteins. Compared traditional methods, method possesses some remarkable features follows: 1) by virtue PS-SDP reaction, a aptamer assembly binding trigger 1 2 was functional structure for simultaneous sensing ATP lysozyme. 2)...

A novel surface-enhanced Raman scattering (SERS) method is demonstrated to detect L-histidine based on DNAzyme which can specifically recognize L-histidine. Through a target recycling cascade amplification strategy, the DNAzyme-based SERS sensing system affords very high sensitivity for with detection limit of 0.56 nM.

Simultaneous imaging, diagnosis, and therapy can offer an effective strategy for cancer treatment. However, the complex probe design, poor drug release efficiency, multidrug resistance remain tremendous challenges to Here, a novel one–two–three system is built enhanced imaging detection of miRNA-21 (miR-21) overexpressed in cell chemogene therapy. The consists dual-mode DNA robot nanoprobes assembled by two types hairpin DNAs three-way branch modified on gold nanoparticles, with...

The diagenesis and porosity evolution of the Middle Jurassic Shaximiao sandstones were analyzed based on petrographic observations, X-ray diffractometry, scanning electron microscopy carbon oxygen stable isotope geochemistry, fluid inclusion microthermometry, thermal burial history modeling results. point count data show that secondary pores (av. 5.5 %) are more abundant than primary 3.7 thus dominant pore type in sandstones. Analysis indicates alteration mainly occurred during two...

A symmetric signal amplification (SSA) assay using a dual functional DNA–linker–DNA (DLD) probe is fabricated to simultaneously detect miRNAs.