Simultaneous detection of cancer biomarkers holds great promise for the early diagnosis different cancers. However, in presence high-concentration biomarkers, signals lower-expression are overlapped. Existing techniques not suitable simultaneously detecting multiple at concentrations with significantly orders magnitude. Here, we propose an asymmetric signal amplification method levels. Using bifunctional probe, a linear mode responds to markers, and quadratic low-concentration markers. With...

Abstract The endocytic and autophagic pathways play important roles in abiotic stress responses maintaining cellular homeostasis plants. Asparagine Rich Proteins (NRPs) are plant-specific stress-responsive proteins that involved many stress-related signaling pathways. We previously demonstrated NRP promotes PIN FORMED 2 (PIN2) vacuolar degradation to maintain PIN2 under abscisic acid (ABA) treatment Arabidopsis (Arabidopsis thaliana). However, the molecular function mechanism of vesicle...

Reversal learning has been widely used to probe the implementation of cognitive flexibility in brain. Previous studies monkeys identified an essential role orbitofrontal cortex (OFC) reversal learning. However, underlying circuits and molecular mechanisms are poorly understood. Here, we use T-maze investigate neural mechanism olfactory Drosophila . By adding a training cycle classical protocol, show that wild-type flies able reverse their choice according alteration conditioned stimulus...

A novel micro/nano-composite, based on cobalt(II) tetranitrophthalocyanine (CoTNPc) grown poly(sodium-p-styrenesulfonate) modified graphene (PGr), as a non-noble-metal catalyst for the oxygen reduction reaction (ORR), is fabricated by an in situ solvothermal synthesis method. The CoTNPc/PGr characterized scanning electron microscopy (SEM), transmission (TEM), ultraviolet-visible (UV-vis) absorption spectroscopy, Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron (XPS),...

Abstract Cap-adjacent nucleotides of animal, protist and viral mRNAs can be O -methylated at the 2‘ position ribose (cOMe). The functions cOMe in animals, however, remain largely unknown. Here we show that two cap methyltransferases (CMTr1 CMTr2) Drosophila methylate first nucleotide mRNA. Double-mutant flies lack but are viable. Consistent with prominent neuronal expression, they have a reward learning defect rescued by conditional expression mushroom body neurons before training. Among...

Inflexible cognition and behavior are prominent features of prefrontal cortex damage several neuropsychiatric disorders. The ability to flexibly adapt cognitive processing dynamically changing environmental contingencies has been studied using the reversal learning paradigm in mammals, but complexity brain circuits precludes a detailed analysis underlying neural mechanism. Here we study circuitry mechanism supporting flexible genetically tractable model organism, Drosophila melanogaster ....

A novel surface-enhanced Raman scattering (SERS) detection system is developed for proteins and nucleic acids based on a triple-helix molecular switch multiple cycle signal amplification, achieving high sensitivity, universality, rapid analysis, selectivity.

A dopamine (DA) sensor based on a methoxypolyethylene glycol (MPEG) polymer covalently modified glass carbon electrode (GCE) was fabricated by an electroadsorption method. The electrochemical behavior of the towards catalytic oxidation DA in pH 5.0 phosphate buffer solution (PBS) investigated cyclic voltammetry. obviously enhanced current response and decreased overpotentials for DA. Using differential pulse voltammetry, gave linear to over concentration range 2.0-140 μM with detection limit...

The effects of land-based high-intensity interval training (HIIT) on the cardiovascular system have already been demonstrated. However, water environment is different from that land. Therefore, we investigated 6-week aquatic HIIT and moderate-intensity continuous (MICT) central hemodynamic parameters, endothelial function, aerobic fitness in inactive adults. Thirty-one adults were randomly assigned to or MICT group. group performed twelve 30-s swimming exercise bouts with intensity 95% HRmax...

A stable electroactive film of β-cyclodextrin-poly(N-isopropylacrylamide) (CD-PNIPAM) was successfully prepared on a glassy carbon electrode (GCE) surface using an electroadsorption method for the determination dopamine (DA). The electrochemical behavior fabricated sensor towards catalytic oxidation DA investigated by cyclic voltammetry (CV) in pH 5.0 phosphate buffer solution. CD-PNIPAM modified exhibited good electrocatalytic activity dopamine, and led to significant decrease anodic...

Abstract Introduction Major depressive disorder (MDD) is one of the most common mental disorders worldwide. The aim this study was to identify potential pathological genes in MDD. Methods We searched and downloaded gene expression data from Gene Expression Omnibus database differentially expressed (DEGs) Then, Kyoto Encyclopedia Genes Genomes pathway, Ontology analysis, protein‐protein interaction (PPI) network were applied investigate biological function identified DEGs. quantitative...

How plants balance growth and stress adaptation is a long-standing topic in plant biology. Abscisic acid (ABA) induces the expression of stress-responsive Asparagine Rich Protein (NRP), which promotes vacuolar degradation PP6 phosphatase FyPP3, releasing ABI5 transcription factor to initiate transcription. Whether NRP required for remains unknown. We generated an nrp1 nrp2 double mutant, had dwarf phenotype that can be rescued by inhibiting auxin transport. Insufficient transition zone...

Dietary magnesium (Mg 2+ ) supplementation can enhance memory in young and aged rats. Memory-enhancing capacity was largely ascribed to increases hippocampal synaptic density elevated expression of the NR2B subunit NMDA-type glutamate receptor. Here we show that Mg feeding also enhances long-term Drosophila . Normal -enhanced fly appears independent NMDA receptors mushroom body instead requires a conserved CNNM-type -efflux transporter encoded by unextended ( uex gene. UEX contains putative...

Gout can affect the quality of life patients due to monosodium urate monohydrate (MSU) crystals. Numerous studies have proposed that long noncoding RNAs (lncRNAs) regulate gout. We aimed reveal function lncRNA small nucleolar RNA host gene 8 (SNHG8) in acute gouty arthritis (GA). A GA mouse model was established by injection MSU into footpads. The levels SNHG8, miR-542-3p and adaptor-related protein complex 3 subunit delta 1 (AP3D1) footpads were detected via polymerase chain reaction...

Tool-chip friction increases cutting temperature, aggravates tool wear, and shortens the service life of tools. A micro-groove design rake face can improve wear performance tool. In this study, we used finite element simulation “Deform” to obtain temperature field distribution face. The size was determined by selecting a suitable combined with characteristics tool–chip flow in process, prepared using powder metallurgy. three-direction forces tip were obtained test. Compared original turning...

Gouty arthritis (GA) is mainly caused by the precipitation of monosodium urate (MSU) crystals in joint. Recently, different regulatory roles microRNAs (miRNAs) have been widely verified. Nevertheless, specific function microRNA-486-5p (miR-486-5p) GA still unclear. cell models vitro were established treatment 250 μg/mL MSU into THP-1 cells or J774A.1 cells. Then, accumulation tumor necrosis factor (TNF)-α, interleukin (IL)-8, and IL-β was estimated ELISA. The mRNA levels TNF-α, IL-8,...

Gouty arthritis (GA) is caused by monosodium urate (MSU) crystal accumulation in the joints. MSU-mediated inflammation an important inducing factor gouty (GA). Recent studies have demonstrated that microRNAs can influence GA progression. Herein, role and mechanism of miRNA-142-3p were explored. To establish vitro vivo models, MSU was used to induce inflammatory response human monocyte cell line THP-1 male C57BL/6 mice. Protein levels, gene expression proinflammatory cytokine secretion...