ABSTRACT Introduction: The minced buccal mucosal graft endourethral urethroplasty (MBGEU) is our novel technique of combining direct vision internal urethrotomy with urethroplasty. We assessed success at 30-months follow-up. Secondary objectives were to compare the changes in American Urological Association (AUA) symptom score, peak flow rate (Qmax), and post-void residue (PVR) postoperatively. Methods: This pilot study (CTRI/2021/09/036651) was conducted a tertiary-care center included 30...

Cell cycle regulator genes are major target of mutation in many human malignancies including glioblastomas (GBMs). CDKN2A is one such tumor suppressor gene which encodes p16INK4a protein and serves as an inhibitor cell progression. Very few studies available regarding the association deletion with p16 expression GBMs. There limited data on frequency different age groups. The aim present study was to analyze deletions GBM correlate deletional status (i) patient (ii) (iii) other genetic...

Pediatric high‐grade gliomas (HGGs) are highly malignant tumors that remain incurable and relatively understudied. The crucial role of noncoding RNAs (ncRNAs) has been reported in various cancers. However, the study on miRNAs pediatric HGGs is scant there no report till date status other small ncRNAs. Genome‐wide microarray analysis was performed to investigate ncRNA expression HGG ( n = 14) compared adult glioblastoma (GBM) signature. validation nucleolar (snoRNAs) done by real‐time...

Abstract Background Fine‐needle aspiration cytology (FNAC) has been used as the first line approach to lymphadenopathy, which is a common presentation in pediatric age group. The Sydney system for reporting of lymph node (LN) proposed assess reliability, performance, and accuracy procedure. This study intends role FNAC population according system. Methods was retrospective observational from tertiary care center Eastern India. All patients group 0–18 years evaluated during 2016–2024 were...

<title>Abstract</title> Spinal ependymoma and myxopapillary are the two most common spinal ependymal tumor types that feature distinct histological characteristics, genetic alterations DNA methylation profiles. Their distinction may be difficult in individual cases molecular diagnostic assessment, particular methylome profiling, then required to assign correct diagnosis. Expression of homeobox gene HOXB13 at mRNA protein levels has been reported as a frequent finding serve marker for these...

ABSTRACT Introduction Soft tissue lesions encompass a diverse category of diseases from benign to malignant, and their morphology might overlap; therefore, accurate categorization is needed approach the reporting soft cytology. The cytology these helpful in detecting features malignancy, which helps guiding further management. In this study, we applied proposed IAC–IARC–WHO cytopathology system assess risk malignancy (ROM) diagnostic accuracy (DA) for determination its clinical utility....

Pure neuritic leprosy (PNL) is an uncommon form of involving peripheral nerves alone. Some isolated case reports and observational studies have shown imaging changes in the CNS patients with leprosy. This prospective study evaluates involvement nervous system beyond nerve among PNL MR imaging. We screened presenting features neuropathy and/or thickened nerves. Patients were subjected to detailed clinical examination, routine tests along conduction study, biopsy nerve, usually sural nerve....

ABSTRACT Monocarboxylate transporters (MCTs) are crucially implicated in cancer cell metabolism by transporting lactate/H+ ions and thus regulating the pH of microenvironment. We assessed MCT1 MCT4 expression 98 cases adult‐type hemispheric Glioblastomas (GBMs) (IDH wild‐type), along with 51 IDH‐mutant astrocytic oligodendroglial tumors (grade 2–4) for comparison. U87MG LN229 lines were used vitro analysis. Both MCT‐1 MCT‐4 showed significantly higher GBMs on immunohistochemistry than...

Abstract Spinal ependymoma and myxopapillary are the two most common spinal ependymal tumor types that feature distinct histological characteristics, genetic alterations DNA methylation profiles. Their distinction may be difficult in individual cases molecular diagnostic assessment, particular methylome profiling, then required to assign correct diagnosis. Expression of homeobox gene HOXB13 at mRNA protein levels has been reported as a frequent finding serve marker for these tumors. Here, we...

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Enhancer of zeste homolog-2(EZH2) is a key epigenetic regulator that functions as oncogene and also known for inducing altered trimethylation histone at lysine-27 (H3K27me3) mark in various tumors. However, H3K27me3 targets their precise relationship with gene expression are largely unknown astrocytic In this study, we checked EZH2 messenger RNA protein 90 tumors different grades using quantitative PCR immunohistochemistry, respectively. Further, genome-wide ChIP-seq analysis modification...

&lt;b&gt;&lt;i&gt;Introduction:&lt;/i&gt;&lt;/b&gt; The Milan System for Reporting Salivary Gland Cytopathology (MSRSGC) was proposed by the American Society of and International Academy Cytology to bring uniformity in reporting system treatment protocol. A wide range risk malignancy each category has been reported various authors applying system. &lt;b&gt;&lt;i&gt;Aim:&lt;/i&gt;&lt;/b&gt; We intend study cytohistological concordance ROM diagnostic categories &lt;b&gt;&lt;i&gt;Materials...

Abstract Pilocytic astrocytomas occur rarely in adults and show aggressive tumor behavior. However, their underlying molecular‐genetic events are largely uncharacterized. Hence, 59 adult pilocytic astrocytoma (APA) cases of classical histology were studied (MIB‐1 LI: 1%–5%). Analysis BRAF alterations using qRT‐PCR, confirmed KIAA1549‐BRAF fusion 11 (19%) BRAF‐gain 2 (3.4%) cases. BRAF‐V600E mutation was noted 1 (1.7%) case by sequencing. FGFR1‐mutation FGFR‐TKD duplication seen 7/59 (11.9%)...

Gliosarcoma is a rare variant of glioblastoma multiforme (GBM) with similar clinical presentation and prognosis but distinct genetic profile. The clinicopathological features 22 cases gliosarcoma were analyzed respect to age, sex, KPS score, operative diagnosis, extent resection histopathological subtype (predominantly sarcomatous [PS], predominantly gliomatous [PG] or mixed). Twelve PS, six PG four mixed. histological did not correlate the diagnosis; however, it significantly (P=0.014). In...

Poorly differentiated thyroid carcinoma (PDTC) is an uncommon malignancy with biological behaviour intermediate between well-differentiated and undifferentiated carcinoma. The cytological diagnosis of PDTC often difficult as a result lack well-established cytomorphological features considerable degree morphological overlap other commoner neoplasms.To review the aim highlighting salient diagnostic differential problems.Seven cases histologically proven available aspiration cytology smears...

Molecular and clinical characteristics of pediatric meningiomas are poorly defined. Therefore, we analyzed clinical, morphological molecular profiles meningiomas. Forty from January 2002 to June 2015 were studied. 1p36, 14q32 22q-deletion assessed by fluorescent in situ hybridization mutations most relevant exons AKT, SMO, KLF4, TRAF pTERT using sequencing. Expression GAB1, stathmin, progesterone receptor (PR), p53 along with MIB-1 LI was examined immunohistochemistry. There 36 sporadic four...

Enhancer of zeste homolog 2 ( EZH2 ) mediated down‐regulation CDKN2A /p16 has been observed in cell lines as well a few carcinomas. However, there is no study correlating expression with status gliomas. Hence, the present was conducted to evaluate astrocytic and oligodendroglial tumors correlate MIB ‐1 labeling index LI ). Gliomas all grades n = 118) were studied using immunohistochemistry assess , p16 fluorescence situ hybrization gene status. homozygous deletion HD both significantly more...

Although role of individual microRNAs (miRNAs) in the pathogenesis gliomas has been well studied, their as a clustered remains unexplored gliomas.In this study, we performed expression analysis miR-379/miR-656 miRNA-cluster (C14MC) oligodendrogliomas (ODGs) and also investigated mechanism underlying modulation cluster.We identified significant downregulation majority miRNAs from cluster ODGs. Further data The Cancer Genome Atlas (TCGA) confirmed global C14MC. Furthermore, observed that its...

This study aims (1) to evaluate ATRX expression in different grades and subtypes of gliomas correlate with other hallmark genetic alterations, (2) identify characterize mosaic/heterogeneous staining terms mutation status. One hundred seventy six cases glioma were assessed for immunohistochemistry subdivided into positive, negative patterns. Five heterogeneous further subjected next generation sequencing. Higher frequency immune-negativity was detected grade II/III astrocytic, oligoastrocytic...