Abstract Corona Virus Disease 2019 (COVID-19) is a recently emerged life-threatening disease caused by SARS-CoV-2. Real-time fluorescent PCR (RT-PCR) the clinical standard for SARS-CoV-2 nucleic acid detection. To detect early and control spreading on time, faster more convenient method detecting, RT-LAMP (reverse transcription loop-mediated isothermal amplification) was developed. RNA reverse amplification were performed in one step at 63 °C conditions, results can be obtained within 30...

Melophagus ovinus is considered to be of great veterinary health significance. However, little known about the information on genetic mechanisms specific biological characteristics and novel methods for controlling M. ovinus.

Abstract Background Influenza A virus (IAV) can cause severe and life-threatening illness in humans animals. Therefore, it is important to search for host antiviral proteins elucidate their mechanisms the development of potential treatments. As a part human innate immunity, restriction factors inhibit replication viruses, among which SAM HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1 (SAMHD1) restrict such as HIV enterovirus EV71. Viruses also developed...

The integrated ducted structure significantly enhances the aerodynamic performance of duct–rotor system, reducing noise and enhancing rotor safety. However, in coupled parameters duct are crucial to its characteristics. This study employs a Computational Fluid Dynamics numerical analysis investigate duct–rotor, focusing on related blade’s relative position tip gap. Additionally, our research delves into fundamental mechanisms key parameters, such as blade gap, elucidating their role...

MicroRNAs (miRNAs) are key regulators of gene expression, exerting post-translational control through mRNA silencing or degradation. These molecules play pivotal roles in host–pathogen interactions, particularly modulating antiviral immune responses. The global public health threat posed by the H5N1 Highly Pathogenic Avian Influenza (HPAI) virus necessitates urgent exploration novel therapeutic strategies. Our investigation revealed significant dysregulation miR-18a-5p following influenza...

Alpha-fetoprotein (AFP)-producing gastric cancer (AFPGC), represented by the production of AFP, has a more aggressive behavior than common cancer. The underlying mechanisms are not well understood. Arsenic trioxide (As2O3) is used clinically to treat acute promyelocytic leukemia(APL) and activity in vitro against several solid tumor cell lines, with induction apoptosis inhibition proliferation prime effects. Signal transducer activator transcription 3 (STAT3) an important role tumorigenesis...

Preeclampsia is a major reason of morbidity and mortality in pregnant women perinatal fetus. Hence, it prime importance that diagnostic markers are defined to predict chances preeclampsia women. It has been previously shown microRNA (miRNA)-376c expression decreased the placenta patients at term. Even though this decrease was not mimicked pre-term stage, miR-376c plasma these as early second trimester. Plasma specimens were obtained from having unifetal gestation undergoing care between...

Melophagus ovinus (sheep ked) is a hematophagous ectoparasite that mainly parasitizes sheep. In addition to causing inflammation, wool loss, and skin damage the animal hosts, M. also serves as vector for variety of pathogens highly likely participate in life transmission cycle pathogenic organisms. Herein, we investigated presence molecular characterization vector-borne from Qinghai-Tibet Plateau, China. A total 92 pools collected Qinghai province China were screened selected pathogens. The...

The effect of cyclic serious/medium hypoxia stress in the presence environment on shrimp is not well explored. survival, growth performance, osmoregulation gene expression, digestive enzyme activity, histology, and resistance against Vibrio parahemolyticus white Litopenaeus vannamei reared under conditions (CSMH, 0.8 - 3.5 mg/L) versus normoxia (N, 6.4 7.5 were investigated during an experimental period 28 days. Consequently, cumulative mortality rate CSMH increased continuously. weight gain...

Infection with H5N6 highly pathogenic avian influenza virus caused high mortality in chickens, while ducks often appear to be asymptomatic. But, some recent H5Nx subtype viruses could cause ducks. The variation between different species and the mechanisms by which death requires investigation identify key processes susceptibility pathogenesis. Here, we characterized two representative viruses, A/ Pavo cristatus /Jiangxi/JA1/2016 (JA1) Anas crecca /shanghai/SH1/2016 (SH1), compared their...

The sex-linked short tandem repeats (STR), Y-STR and X-STR, are important for autosomal STRs in forensic paternity testing. We evaluated the parameters of 19 Y-STRs 16 X-STRs Han population Shandong province, China. A Goldeneye 20Y kit (DYS391, DYS389I, DYS390, DYS389II, DYS348, DYS456, Y-GATA-H4, DYS447, DYS19, DYS392, DYS393, DYS388, DYS439, DYS635, DYS448, DYS460, DYS458, DYS437, DYS385 a/b) was used to analyze 534 unrelated males. Goldeneye17X system (DXS6795, DXS9902, DXS8378, HPRTB,...

The abundance and prevalence of parasitic infection often vary in different host sexes, this phenomenon has been named sex-biased parasitism. Brandt's voles are the dominant rodent species typical steppe habitat widely distributed Inner Mongolia, China, but parasites poorly reported. In study, we investigated six intestinal May, June, July, August 2022 around Xilingol Grassland China. results showed that Syphacia obvelata, Aspiculuris tetraptera, Trichostrongylidae family were captured rates...

Rhesus macaques (Macaca mulatta) are hosts to a range of zoonotic and potentially pathogens. The present study firstly provides broader investigation the presence prevalence fecal pathogens in wild Taihangshan macaques, subspecies rhesus macaque China. A total 458 samples were collected between September 2015 November 2016. Fourteen genera intestinal parasites (four protozoans ten helminths) twelve bacteria tested for using PCR amplification. overall parasitic infection was 98.25%. Entamoeba...

Tick-borne diseases (TBDs) can cause serious economic losses and are very important to animal public health. To date, research on TBDs has been limited in Qinghai-Tibet Plateau, China. This epidemiological investigation was conducted evaluate the distribution risk factors of Anaplasma spp. Ehrlichia chaffeensis livestock Qinghai. A total 566 blood samples, including 330 yaks (Bos grunniens) 236 Tibetan sheep (Ovis aries) were screened. Results showed that A. bovis (33.3%, 110/330)...

In October 2021, a wild bird-origin H3N8 influenza virus-A/Chinese pond heron/Jiangxi 5-1/2021 (H3N8)-was isolated from Chinese heron in China. Phylogenetic and molecular analyses were performed to characterize the genetic origin of strain. analysis revealed that eight gene segments this avian virus belong Eurasian lineages. HA clustered with viruses is circulating poultry southern The NA possibly originated ducks South Korea has highest homology (99.3%) A/Wild duck/South...

Female mice can discriminate the urinary odors of male due to their olfactory acuity. Parasitic infection or subclinical decrease odor attractiveness and finally lead aversion avoidance responses in selection for female mice. Trichinella spiralis is a kind tissue-parasitizing nematode that causes trichinellosis, zoonotic parasitic disease spreads throughout world. However, reproductive injury caused by was not fully revealed. In this study, we explored effect on capacity ICR/CD-1 We...

Contracaecum species are economically important fish-borne larval nematodes with zoonotic significance. In June 2019, more than 100 piscivorous birds died in their habitats close to the Wild Duck Lake, located Yanqing, northwest of Beijing, China. Post-mortem examination Black Night Herons (Nycticorax) revealed presence numerous anisakid proventriculus. Recovered were identified as sp. based on morphological description. Phylogenetic analysis mitochondrial (mt) genome and ITS gene showed...

To analyse the genetic polymorphisms of 19 autosomal STR loci in Han population east, middle-northwest and southwest-south Shandong to explore its relationships among these three regions.STR 1 044 unrelated individuals regions were typed with a Goldeneye® DNA ID System 20A kit. The allele frequency genetics parameters statistically analysed by Modified-Powerstates software. distances calculated Arlequin v3.5 phylogenetic tree was conducted using MEGA v4.0 software.Fifteen detected H values...

H11N9 viruses in wild birds might have provided the NA gene of human H7N9 virus early 2013 China, which evolved with highly pathogenic strains 2017 and caused severe fatalities. To investigate prevalence evolution influenza viruses, 16,781 samples were collected analyzed during 2016-2020. As a result, novel strain A (H11N9) several characteristics that increase virulence was isolated. This had reduced pathogenicity chicken mice able to replicate without prior adaptation. Phylogenetic...

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly pathogenic to humans and has caused the ongoing disease 2019 (COVID-19) pandemic. Vaccines are one of efficient ways prevent viral infection. After COVID-19 vaccination, monitoring dynamic change in neutralizing antibodies necessary determine booster requirements.We estimated effectiveness inactivated vaccines by SARS-CoV-2 for over years. Additionally, we also investigated activation T lymphocytes (CD3+ cells) after...