A5077387427- Cerebrovascular and Carotid Artery Diseases
- Intracranial Aneurysms: Treatment and Complications
- Glaucoma and retinal disorders
- Cardiovascular Health and Disease Prevention
- Acute Ischemic Stroke Management
- Retinal Imaging and Analysis
- Atherosclerosis and Cardiovascular Diseases
- Peroxisome Proliferator-Activated Receptors
- Protease and Inhibitor Mechanisms
- Eicosanoids and Hypertension Pharmacology
- Heme Oxygenase-1 and Carbon Monoxide
- Inflammatory mediators and NSAID effects
- Cancer, Lipids, and Metabolism
- Cerebrovascular and genetic disorders
- Cholesterol and Lipid Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Neurological Disease Mechanisms and Treatments
- Caveolin-1 and cellular processes
- Cardiac Imaging and Diagnostics
- Congenital heart defects research
- Signaling Pathways in Disease
- Immune cells in cancer
- Intracerebral and Subarachnoid Hemorrhage Research
- Cardiac Fibrosis and Remodeling
- Neuroinflammation and Neurodegeneration Mechanisms
Helsinki University Hospital
2016-2025
University of Helsinki
2015-2025
Wihuri Research Institute
2003-2014
Institute for Molecular Medicine Finland
2014
Finnish Institute for Health and Welfare
2014
Finnish Red Cross
2014
University of Tartu
2014
South Karelia Central Hospital
2006-2007
Swedish Orphan Biovitrum (United States)
2006
Neurology, Inc
2006
Abstract Background and purpose Perioperative stroke is a well‐recognized complication of carotid endarterectomy (CEA), but well‐performing prediction models do not exist for it. Our aim was to identify novel predictors perioperative ischaemic cerebrovascular events (iCVEs), emphasizing imaging potential biomarkers in stenosis (CS) patients well‐characterized prospective CS cohort. Methods Helsinki Carotid Endarterectomy Study 2 an observational consecutive cohort study subjected CEA during...
Objective— We studied by microarray analysis whether symptomatic and asymptomatic carotid plaques from the same patient differ in gene expression changes are present an independent sample set. Methods Results— Carotid four patients with bilateral high-grade stenosis, one being other asymptomatic, were analyzed on Affymetrix U95Av2 arrays. 33 genes showed >1.5-fold change between intraindividual comparison FDR ranging 0.28 to 0.40. Three involved iron-heme homeostasis, CD163, HO-1,...
Adipophilin is an adipose differentiation-related protein expressed in lipid-containing cells. Using DNA microarray analysis, we previously found the adipophilin gene (ADFP) to be overexpressed symptomatic carotid plaques (CP). This led us further examine role of atherosclerosis relative symptom status.Ninety-eight high-grade (>70%) CPs were obtained endarterectomy. The expression ADFP mRNA was measured by quantitative real-time RT-PCR, and amount quantified with Western blotting. Detailed...
Background: Carotid endarterectomy (CEA) has been associated with both postoperative cognitive dysfunction (POCD) and improvement (POCI). However, the prognostic significance of changes related to CEA is largely unknown. The aim this study was examine associations between after long-term survival. Methods: We studied 43 patients 1 day before as well 4 days 3 months surgery an extensive neuropsychological test array, followed them for up 14 years. POCD POCI relative baseline were determined...
Atherosclerotic carotid stenosis is an important risk factor for stroke. Carotid plaques (CPs) causing stroke may present a distinct type of molecular pathology compared with transient ischemic attack (TIA)-associated or asymptomatic plaques. We the gene expression profiles CPs from patients (n = 12) and 9), both similar factors severity (>70%). Sixty probes showed over 1.5-fold difference at 5% false discovery rate. Functional clustering enrichment genes in 51 GO categories seven pathways,...
<b><i>Background:</i></b> Prior studies have suggested a central role for cellular adhesion molecules (CAMs) in the pathophysiology and symptoms of atherosclerotic carotid plaques (CPs). <b><i>Objective:</i></b> This study examined CAMs symptom generation patients with advanced artery disease. <b><i>Methods:</i></b> Ninety-two consecutive underwent endarterectomy, six both sides (54 symptomatic 41 asymptomatic CPs). Intercellular molecule-1 (ICAM-1), vascular cell (VCAM-1), P-selectin,...
Background— CD36 is a macrophage scavenger receptor mediating the uptake of modified lipoproteins, whereas ABCA1 transporter counteracts this effect by cellular lipid efflux. Based on DNA microarray, we previously found that and genes were overexpressed in symptom-causing carotid plaques (CP) compared with nonsymptom-causing CP. To evaluate their role CP destabilization, conducted detailed immunohistochemical studies localization lipids, proteins, extravasated red blood cells,...
Abstract Purpose To compare subfoveal choroidal thickness (SFCT) and associated clinical variables in patients with carotid stenosis (CS) before 6 months after endarterectomy (CEA). Methods The prospective non‐randomized Helsinki Carotid Endarterectomy Study – Brain Eye Sub‐sTudy included seventy (81% male, mean age 69 years) 40 control subjects (77% 68 years), from March 2015 to December 2018. Ophthalmological examination SFCT measured enhanced‐depth imaging‐optical coherence tomography....
Background Atherosclerosis affects several vascular trees systemically and though surgical plaque removal diminishes the risk of stroke in patients with carotid stenosis, they still face a other atherothrombotic complications like myocardial infarction premature death. Aims and/or hypothesis This study was designed to reveal long-term death events following endarterectomy. Methods Eighty-nine previously (1997–2000) endarterectomized (56–92 years) were followed up 15·2 years. Causes death,...
To assess retinal findings in patients with severe carotid stenosis (CS) before and after endarterectomy (CEA) compared to those controls.This study is based on 70 (male 81%, mean age 69) scheduled for CEA Helsinki University Hospital 41 healthy nonmedicated controls 76%, 68). Our examinations included fundus photographs. Semi-automated software (Vesselmap, Imedos) served evaluation of central arterial equivalent (CRAE) venular (CRVE), arterio-venous ratio (AVR) both eyes. We assessed...
Introduction: Every fifth ischemic stroke is caused by thromboembolism originating from an atherosclerotic carotid artery plaque. While prevention the most cost-effective therapy, antiplatelet and cholesterol-lowering drugs have a ceiling effect in their efficacy. Therefore, discovery of novel pathophysiologic targets are needed to improve primary secondary stroke. This article provides detailed study design protocol HeCES2, observational prospective cohort with objective investigate...
Background: Long-term treatment with the vitamin K antagonist warfarin is widely used for prevention of venous thrombosis and thromboembolism. However, antagonists may promote arterial calcification, a phenomenon that has been previously studied in coronary peripheral arteries, but not extracranial carotid arteries. In this observational cohort study, we investigated whether associated calcification atherosclerotic Methods: Overall, 500 consecutive patients underwent endarterectomy, 82 whom...
Glutamine synthetase (GLUL), the sole generator of glutamine, is a metabolic nexus molecule also involved in atherosclerosis. We recently demonstrated 2.2-fold upregulation GLUL mRNA stroke-causing carotid plaques when compared with from asymptomatic patients. Here we same cohort expression plaque gross morphology, and colocalization immunodetectable protein histopathological changes molecular mechanical mediators linked to development.Endarterectomy specimens 19 24 stroke patients were...
<i>Background:</i> We examined the hypothesis that endothelial denudation in advanced carotid plaques (CPs) occurs by increased apoptosis of cells (ECs) using scanning electron microscopy (SEM) as well markers cellular proliferation and symptomatic CPs (SCPs) asymptomatic (ACPs). <i>Methods:</i> 93 consecutive patients underwent endarterectomy. Five additional specimens were studied SEM. performed TUNEL assays, immunostaining against Fas receptor (FasR), ligand...
<h2>Abstract</h2><h3>Background</h3> Carotid endarterectomy (CEA) has been associated with both cognitive decline and improvement, but the underlying neurovascular mechanisms are unclear. The aim of this study was to investigate relationship between indices changes after CEA. <h3>Methods</h3> We studied 55 patients severe (≥70%) symptomatic or asymptomatic carotid stenosis before six months A wide array neuropsychological tests arranged in eight domains functions specific hemisphere...
We describe hypoperfusion-related and embolic ocular signs of carotid stenosis (CS) before six months after endarterectomy (CEA) in a CS population.We enrolled prospectively 70 CEA patients (81% male, mean age 69) 41 non-medicated control subjects (76%, 68), from March 2015 to December 2018, assessing intraocular pressure (IOP), best-corrected visual acuity (BCVA) logMAR units performing bio-microscopy examination.Main index symptoms included amaurosis fugax (Afx) (29, 41%) hemispheric TIA...
Retinal vascular function was assessed in patients with carotid stenosis (CS) before and six months after endarterectomy (CEA) controls at a six-month interval.We studied 68 (81% male, mean age 69) 41 healthy non-medicated (77%, 68) from March 2015 to December 2018. Our ophthalmological examination included flicker-induced arteriolar venular measurements Dynamic Vessel Analyser both eyes.At baseline, dilation reduced the ipsilateral eyes of compared (arteriolar 1.0% versus 2.6%, p = 0.001...
Purpose To assess ophthalmic findings of ipsilateral (IL) and contralateral (CL) eyes 30 first patients undergoing endarterectomy for carotid artery disease in Helsinki Carotid Endarterectomy Study 2‐ Substudy (HeCES‐BEST) (inclusion criteria ≥70% occlusion, exclusion unable to undergo neuropsychological tests or MRI) Methods Best corrected visual acuity (BCVA) with ETDRS chart at 4 meters, anterior posterior segment biomicroscopy, 30°/50°/200° fundus photography. Differences proportions...