A5050738002- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- Platelet Disorders and Treatments
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Blood transfusion and management
- Immune cells in cancer
- Cancer Research and Treatments
- Blood disorders and treatments
- Colorectal Cancer Surgical Treatments
- Transplantation: Methods and Outcomes
- Renal Transplantation Outcomes and Treatments
- Liver Disease and Transplantation
- Hepatitis Viruses Studies and Epidemiology
- Nutrition and Health in Aging
- Cardiac and Coronary Surgery Techniques
- Liver Diseases and Immunity
- RNA Interference and Gene Delivery
- Polyamine Metabolism and Applications
- Parvovirus B19 Infection Studies
- Neutropenia and Cancer Infections
- Enhanced Recovery After Surgery
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Acute Myeloid Leukemia Research
Johannes Gutenberg University Mainz
2012-2023
University Medical Center of the Johannes Gutenberg University Mainz
2012-2023
Deutsche Klinik für Diagnostik
2004-2012
In the tumor microenvironment, arginine is metabolized by arginase-expressing myeloid cells. This depletion profoundly inhibits T cell functions and crucially involved in tumor-induced immunosuppression. Reconstitution of adaptive immune context arginase-mediated escape a promising therapeutic strategy to boost immunological anti-tumor response. Arginine can be recycled certain mammalian tissues from citrulline via argininosuccinate two-step enzymatic process involving enzymes synthase (ASS)...
Myeloid cell arginase-mediated arginine depletion with consecutive inhibition of T functions is a key component tumor immune escape. Both, granulocytic myeloid-derived suppressor cells (G-MDSC) and conventional mature human polymorphonuclear neutrophil granulocytes (PMN) express high levels arginase 1 can act as adaptive anti-cancer immunity. Here we demonstrate that pharmacological PMN-derived not only prevents the suppression but rather leads to strong hyperactivation cells. Human PMN were...
The amino acid arginine is fundamentally involved in the regulation of immune response during infection, inflammatory diseases and tumor growth. Arginine deficiency (e.g. due to myeloid cell enzyme arginase) inhibits proliferation effector functions activated T lymphocytes. Here, we studied intracellular mechanisms mediating this suppression human Our proteomic analysis revealed an impaired dephosphorylation actin-binding protein cofilin upon T-cell activation absence arginine. We show that...
Tumor-growth is often associated with the expansion of myeloid derived suppressor cells that lead to local or systemic arginine depletion via enzyme arginase. It generally assumed this deficiency induces a global shut-down T cell activation ensuing tumor immune escape. While impact on polyclonal proliferation and cytokine secretion well documented, its influence chemotaxis, cytotoxicity antigen specific human has not been demonstrated so far. We show here chemotaxis early calcium signaling...
The aim of this study was to evaluate risk factors for red blood cell (RBC) transfusion in non-cardiac thoracic surgery.All patients undergoing surgery a single tertiary referral center between January and December 2021 were eligible study. Data on requests perioperative RBC retrospectively analyzed.A total 379 included, whom 275 (72.6%) underwent elective surgery. overall rate 7.4% (elective cases: 2.5%, non-elective 20.2%). Patients with lung resections required 2.4% the cases versus 44.7%...
Perioperative red blood cell (RBC) transfusions have been associated with increased morbidity and worse oncological outcome in some solid neoplasms. In order to elucidate whether RBC themselves, the preoperative anemia of cancer (AOC), or impaired global health status might explain this impact on patients endometrial (EC) ovarian (OC), we performed a retrospective, single-institution cohort study. Women older than 60 years EC OC were included. The influence transfusions, AOC, frailty...
Abstract Background Hepatitis E virus (HEV) infection is a potential reason for elevated liver enzymes after transplantation (LT). Our aim was to analyze real‐world cohort of LT patients, who underwent biopsy transaminases and suspected acute rejection, evaluate frequency post‐transplant HEV infection. Patients Data from 160 biopsies were analyzed. Seventy‐one patients biopsied on schedule without enzymes. A subgroup 25 with rejection chosen further analysis. Patient demographics data...
Ischemic-type biliary lesions (ITBL) are the most troublesome complications after liver transplantation. Their cause remains unknown and, although some risk factors have been identified, results from different research groups often conflicting. The goal of this study was to investigate potential for ITBL.565 transplantations performed between September 1997 and August 2010 were identified divided into two cohorts: 77 in which patient developed ITBL 488 no occurred. following analyzed: donor...
To facilitate preclinical testing of T-cell receptors (TCRs) derived from tumor-reactive clones it is necessary to develop convenient and rapid cloning strategies for the generation TCR expression constructs. Herein, we describe a pDONR™221 vector backbone allowing generate Gateway™ compatible entry encoding optimized bicistronic αβTCR It harbors P2A-linked constant regions head-to-head-oriented recognition sites Type IIS restriction enzymes BsmBI BsaI seamless TCRα TCRβ V(D)J regions,...
Additional Supporting Information may be found in the online version of this article at publisher's website. Appendix S1. Supplemental material. Table D antigen density (D antigens per red blood cell). S2. Serologic reactivity with panels anti-D. Fig. Nucleotide sequencing strategy for RHD and RHCE genes. The nucleotides sequenced are indicated 5'-UTR, 10 exons, 9 introns 3'-UTR gene inversely oriented gene. total number sequences is 5,483 5,851 RHCE. RH haplotypes family. inheritance...