A5000256009- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Biosensors and Analytical Detection
- Anodic Oxide Films and Nanostructures
- Genomics, phytochemicals, and oxidative stress
- Phagocytosis and Immune Regulation
- Thermal properties of materials
- Glycosylation and Glycoproteins Research
- MicroRNA in disease regulation
- Conducting polymers and applications
- GDF15 and Related Biomarkers
- Graphene and Nanomaterials Applications
- Nanopore and Nanochannel Transport Studies
- Microfluidic and Bio-sensing Technologies
- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
Universidad Complutense de Madrid
2021-2025
This work presents the first electroanalytical bioplatforms to track individually or simultaneously at a global level all four methylation marks involved in DNA methylation-demethylation cycle: 5-methylcytosine (5mC) and their sequential oxidative derivatives (5-hydroxymethyl-(5hmC), 5-formyl-(5fC), 5-carboxyl-(5caC) cytosines). The employed direct competitive immunoassay formats implemented on surface of magnetic microparticles (MBs) capture antibodies specific each epimark as well...
Novel amperometric immunoplatform using magnetic micro-supports and screen-printed carbon electrodes, overcoming limitations of the reported methods, to assess senescence-associated GDF-15 marker in diagnosis prognosis colorectal cancer.
The great opportunities represented by exosomes in liquid biopsy diagnostics and the relevance of CD147 protein as diagnostic prognostic cancer biomarker led us to develop first bio-electroanalytical platform for determination exosomal (exoCD147) exploiting micro-sized magnetic beads coated with specific anti-CD147 antibodies. captured target was sandwiched biotin functionalized detector antibodies followed attaching streptavidin-HRP conjugate perform amperometric reading using...
The glycosylation status of proteins is increasingly used as biomarker to improve the reliability in diagnosis and prognosis diseases relevant cancer. This feeds need for tools that allow its simple reliable analysis are compatible with applicability clinic. With this objective mind, work reports first bioelectronic immunoplatforms described date determination glycosylated haptoglobin (Hp) simultaneous total Hp. immunoplatform based on implementation non-competitive bioassays using two...
Pancreatic ductal adenocarcinoma (PDAC) is the solid tumor with worst prognosis, representing today third cause of cancer-related deaths in developed countries and expected to be second 2030. Today, CA19-9 remains only clinically used marker for management PDAC (FDA-approved as a disease monitoring marker). This work reports disposable amperometric immunoplatform determination CA19-9. The skilfully combines advantages magnetic microsupports (MBs) implementation immunoassay transduction on...
Abstract Growth arrest‐specific 6 (GAS6) protein plays a key role in processes related to proliferation, inflammation, angiogenesis, and atherosclerotic plaque formation. In addition, it has been reported that plasma levels of GAS6 are cancer prognosis other relevant pathologies, such as heart failure or sepsis. We report here the first electrochemical immunoplatform for determination GAS6, which demonstrated be competitive with available methodologies terms cost, simplicity, decentralized...