Nisin is an antimicrobial polypeptide produced by Lactococcus lactis and believed nontoxic to humans. The objective of this study was evaluate a nisin-based formulation for the treatment bovine clinical mastitis in lactating dairy cattle. A total 92 cows with 107 clinically mastitic quarters were randomly assigned nisin- (48 51 quarters) gentamicin (GM)-treated (44 56 groups. In nisin-treated group, received intramammary infusion nisin at dose 2,500,000 IU; GM-treated GM administered 0.8 g....

In the large poultry industry, where farmed chickens are fed at high density, prevalence of pathogens and repeated vaccinations induce immune stress, which can significantly decrease production performance increase mortality. This study was designed to shed light on molecular mechanisms metabolic pathways involved in stress through an in-depth analysis transcriptomic metabolomic changes jejunum samples from broilers. Two groups were established for experiment: a control group LPS group....

Previous study showed that ginsenoside Rg1 (Rg1) and Re (Re) alleviated growth inhibition of broiler chicks with immune stress. The aim this was to investigate the effect on inflammatory responses, oxidative stress, apoptosis in liver broilers stress induced by lipopolysaccharide (LPS). Forty were randomly divided into 4 groups, each group consisting 10 chickens. model group, received continuously interval injection 250 μg/kg body weight LPS at age 12, 14, 33, 35 days induce Control injected...

Background: Swine hepatitis E (HEV) is a zoonotic infectious disease caused by the swine virus (SHEV). Open reading frame 3 (ORF3) key virulence factor in HEV, playing crucial role release of viral particles, modulation host innate immune response, and regulation autophagy apoptosis, etc. However, its main function pathogenic mechanism remain incompletely understood. Results: In our study, adenoviruses ADV4-ORF3 ADV4-GFP were successfully constructed mediated overexpression enhanced green...

Aflatoxin B1 (AFB1), a potent mycotoxin, poses significant threat to the poultry industry, particularly affecting health and growth of ducklings. The present study aimed investigate therapeutic effects mechanisms Tianjihuang compound (HRS), traditional Chinese medicine formulation, on AFB1-induced chronic toxicity in Firstly, 30 ingredients, including neochlorogenic acid, kaempferol 3-alpha-D-galactoside, quercetin, hispidulin, caffeic myricetin, were identified from HRS with UPLC-MS/MS...

Broilers with immune stress show decline of growth performance, causing severe economic losses. However, the molecular mechanisms underlying still need to be elucidated. One hundred and twenty broiler chicks were randomly assigned 2 groups 6 repeats per group, 10 birds repeat. The model broilers intraperitoneally injection 250 µg/kg LPS at 12, 14, 33, 35 d age induce immunological stress. Control group was injected an equivalent amount sterile saline. Blood samples from chickens collected...

Schisandra chinensis polysaccharide (SCP) is an experimental therapeutic for the treatment of intestinal injury. Selenium nanoparticle modification can improve bioactivity polysaccharides. In this study, SCP was firstly extracted and purified by a DEAE-52 column, then SCP-Selenium nanoparticles (SCP-Se NPs) were prepared, procedure optimized. Thereafter, obtained SCP-Se NPs characterized transmission electron microscope, X-ray diffraction, energy-dispersive spectroscopy, Fourier transform...

In order to further optimize the purification process of sea buckthorn seed polyphenols and analyze their biological activity, extracts were purified, analyzed identified. parallel, antioxidant properties, antibacterial activity preservation effect purified detected in vitro. The results showed that HPD826 resin had best on free phenol from seed, while AB-8 for bound phenol. And main structure may be prodelphinidins. scavenging activitys DPPH, ABTS hydroxyl radicals, superoxide anions...

Ethnopharmacological relevance Danggui Buxue decoction (DBD) is a traditional Chinese herbal formula. According to the theory of medicine, combination Astragali Radix (AR) and Angelica sinensis (AS) classic prescription tonifying qi enriching blood. DBD has functions hematopoietic, immune enhancement inflammation inhibition, usually used treat blood deficiency symptoms. Aim study Cyclophosphamide (CY) can inhibit humoral cellular immunity, leading overall disorder body, resulting in...

The present study investigated the growth performance, immune status, gut morphology, and microflora modulation in growing-to-finishing pigs (n = 72; 24 pigs/group) after dietary supplementation with Bacillus subtilis (B. subtilis; two groups (experiment 1 (E1) experiment 2 (E2): 63 98 days, respectively) control (Ct, basal diet). results revealed that both 1–98 d 1–63 significantly improved including an increase midtest body weight (MBW) <math xmlns="http://www.w3.org/1998/Math/MathML"...

In broiler chicks , Escherichia coli lipopolysaccharide is a prominent cause for inflammatory damage and loss of immune homeostasis in chicks. Ginsenosides have been shown to anti-inflammatory antioxidant effects. However, it has not demonstrated that ginsenosides protect against stress induced by challenge. The aim this investigate the protective effect Rg1, Re, Rg3 on lipopolysaccharide-induced stress. Our results showed ameliorated growth inhibition fever, as well decreased production...

Abstract This study was conducted to evaluate effects of traditional Chinese medicine formula (TCMF) combined with several herbs on ruminal fermentation, enzyme activities and nutrient digestibility. Twenty finishing bulls were assigned control or different TCMFs (Yufeisan‐1, ‐2, ‐3; 2.5% dry matter (DM) in concentrate). Results showed that DM intake higher ( P &lt; 0.05) the Yufeisan‐3 group than others. Compared control, apparent digestibility crude protein neutral detergent fiber...

Background:Macleaya cordata extract (MCE) is effective in the treatment of enteritis, but its mechanism has not been fully elucidated. Therefore, this study combined network pharmacology and molecular docking technologies to investigate potential pharmacological MCE enteritis. Methods: The information active compounds was accessed through literature. Furthermore, PubChem, PharmMapper, UniProt, GeneCards databases were used analyze targets intersection drug disease imported into STRING...

A recent study has suggested that yeast cell wall product (YP) enhanced serum hemagglutination inhibition (HI) titers and intestinal sIgA responses in chickens immunized with Newcastle disease virus (NDV) vaccine. In the present study, cell-mediated immune elicited by NDV YP were investigated commercial broilers. Broilers fed 0 or 0.1% a live vaccine via an intraocular-and-intranasal route at 14 28 days old. After that, blood samples collected for determination of HI titer, cytokine content,...

The present study was performed to investigate the effect of oral administration β-glucan (G70), a product obtained from cell wall yeast, on Newcastle disease virus (NDV)-specific hemagglutination inhibition (HI) titers, lymphocyte proliferation, and role T subpopulations in chickens treated with live NDV vaccine. In addition, influence splenic gene expression investigated by transcriptome sequencing. results revealed that supplementation boosted titer serum HI increased stimulation index...

Our previous study has demonstrated that administration of ginsenoside Rg3 ameliorates immune stress by inhibiting inflammatory responses, reducing oxidative damage and upregulating mRNA expression mTOR, SOD-1, HO-1. However, the specific mechanism in relation to protective effect on stressed broilers especially metabolites alteration remains obscure. The present aimed investigate underlined pathogenesis using liquid chromatograph-mass spectrometry profiling. Eighteen broiler chicks were...

The conventional procedure for the construction of recombinant expression vector a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming sometimes troublesome because inefficiency ligation. A variety ligase-independent strategies have been developed, but they either involve complicated procedures or need other DNA modifying enzymes. In this study, we report design, an omnipotent pOmni, with can be easily cloned through innovative selection-free...

To investigate the anti-inflammatory activity and mechanism of Baicalin derivative (Baicalin butyl ester, BE).

<title>Abstract</title> Background Porcine hepatitis E is a zoonotic infectious disease caused by swine virus (HEV), open reading frames 3 an important virulence protein of porcine HEV, which plays role in the release viral particles and host innate immune response, regulation autophagy apoptosis, etc., but its main function pathogenic mechanism are not perfect current research. Results In our study, adenoviruses ADV4-ORF3 ADV4-GFP were successfully constructed mediated overexpression...

<title>Abstract</title> Background Brucellosis is a veterinary and human disease caused by <italic>Brucella</italic>, which not only causes serious public safety but also affects the development of animal husbandry international trade. The formyl transferase encoded <italic>wbkC</italic> gene plays an important role in synthesis lipopolysaccharide (LPS), virulence factor <italic>Brucella melitensis</italic>. Long-stranded non-coding RNAs (lncRNAs), as emerging regulatory molecule, are...

Hermetia illucens Larvae Meal (HILM) has been observed to enhance growth performance and immune function, yet the effects mechanisms in geese remain less understood. Experiment I included 64 Sichuan White Geese investigate optimal additive amount of HILM diet, experiment II 32 access serum immunoglobulin, spleen immune-related genes, intestinal morphology gut microbiota at HILM. The results showed that addition 1% significantly increased ADG (p &lt; 0.05), H5-R14 H7-R4 strain titer 33 d...