A5072052429- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Extracellular vesicles in disease
- COVID-19 Clinical Research Studies
- Chemotherapy-induced cardiotoxicity and mitigation
- Tissue Engineering and Regenerative Medicine
- SARS-CoV-2 and COVID-19 Research
- Veterinary Oncology Research
- Viral Infections and Immunology Research
- Escherichia coli research studies
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- GaN-based semiconductor devices and materials
- Microbial infections and disease research
- interferon and immune responses
- Mitochondrial Function and Pathology
- melanin and skin pigmentation
- Pharmacological Receptor Mechanisms and Effects
- Aquaculture disease management and microbiota
- Toxin Mechanisms and Immunotoxins
- Cardiac Structural Anomalies and Repair
- Congenital Heart Disease Studies
- Vector-borne infectious diseases
- Sarcoma Diagnosis and Treatment
Tufts University
2018-2024
New England Disabled Sports
2020
University of Minnesota
2009-2019
University of Minnesota Medical Center
2010
Kansas State University
1982
Mesenchymal stem cells (MSCs) are widely investigated as potential therapeutic agents due to their potent immunomodulatory capacity. Although specific mechanisms by which MSC acts on immune emerging, many questions remain, including the of extracellular vesicles (EVs) mediate biological activities. Canine MSCs interest for both veterinary and comparative models disease have been shown suppress CD4pos T cell proliferation. The aim this study was determine whether EV isolated from canine...
Abstract Background The etiology of hemangiosarcoma remains incompletely understood. Its common occurrence in dogs suggests predisposing factors favor its development this species. These could represent a constellation heritable characteristics that promote transformation events and/or facilitate the establishment microenvironment is conducive for survival malignant blood vessel-forming cells. hypothesis study was characteristic molecular features distinguish from non-malignant endothelial...
Long-term use of doxorubicin (DOX) is limited by cumulative dose-dependent cardiotoxicity.Identify plasma extracellular vesicle (EV)-associated microRNAs (miRNAs) as a biomarker for cardiotoxicity in dogs correlating changes with cardiac troponin I (cTnI) concentrations and, echocardiographic and histologic findings.Prospective study 9 client-owned diagnosed sarcoma receiving DOX single-agent chemotherapy (total 5 treatments). Dogs clinically relevant metastatic disease, preexisting heart or...
Virulent infectious agents such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and methicillin-resistant
Mitral valve prolapse (MVP) is a common valvular disease, affecting 2–3% of the adult human population and degenerative condition. A total 5–10% afflicted will develop severe mitral regurgitation, cardiac dysfunction, congestive heart failure, sudden death. Naturally occurring myxomatous MVP in dogs closely resembles humans structurally, functional consequences are similar. In both species, interstitial cells (VICs) affected valves exhibit phenotype consistent with activated myofibroblasts...
Chemotherapy-induced impairment of autophagy is implicated in cardiac toxicity induced by anti-cancer drugs. Imperfect translation from rodent models and lack vitro has limited investigation autophagic flux dysregulation, preventing design novel cardioprotective strategies based on control. Development an adult heart tissue culture technique a translational model will improve toxicity. We aimed to optimize canine slice system for exploration cancer therapy impact intact tissue, creating...
There is a need to standardize pathologic endpoints in animal models of SARS-CoV-2 infection help benchmark study quality, improve cross-institutional comparison data, and assess therapeutic efficacy so that potential drugs vaccines for can rapidly advance. The Syrian hamster model tractable small COVID-19 clinical disease humans. Using the model, authors used traditional assessment with quantitative image analysis outcomes hamsters administered polyclonal immune sera from previously...
Shiga toxin-producing E. coli (STEC) is a common cause of bloody diarrhea. The pathology STEC infection derives from two exotoxins—Shiga toxin 1 (Stx1) and 2 (Stx2)—that are secreted by in the gut, where they systemically absorbed, causing severe kidney damage leading to hemolytic uremic syndrome (HUS). Currently, there no effective treatment for HUS, only supportive care recommended. We report engineering panel designed ankyrin repeat proteins (DARPin) with potent neutralization activity...
ABSTRACT Hemolytic uremic syndrome (HUS) is a systemic sequelae from gastrointestinal infection with Shiga toxin (Stx) producing Escherichia coli (STEC) that can result in acute kidney injury, lasting renal disease, and death. Despite window for intervention between hemorrhagic diarrhea onset of HUS, no specific therapies exist to prevent or treat HUS following STEC infection. Furthermore, there way predict which patients will develop any rapid determine Stx variant present. To address this,...
Abstract Porcine reproductive and respiratory syndrome virus (PRRSV) is widely considered to be the most important pathogen of swine. Yet, there are no vaccines that produce effective broadly neutralizing antibodies genetically diverse viral genotypes. We recently showed adult sows develop cross-neutralizing following infection outbreaks. However, relevant B-cells producing have not been identified. Thus, we characterized surface markers differentiate porcine B-cell subpopulations determined...
Dogs with cancer treated chemotherapy agents such as doxorubicin (DOX) develop cardiovascular toxicity, providing an opportunity to evaluate cardioprotective strategies in the setting of treatment translatable human disease. However, due lack a suitable approach culture primary adult canine cardiomyocytes, mechanistic interrogation cardiotoxicity after therapy remains challenge. Our study thus aims validate myocardial slice model autophagy modulation and role extracellular vesicle (EV)...
Non-syndromic mitral valve prolapse (MVP), or Barlow’s Disease, is a valvular heart disease with prevalence of 2-3% in the human adult population. Consequences MVP include regurgitation that without surgical treatment can progress to congestive failure, arrhythmias and sudden death. Since degenerative worsens age, risk concern an older population, necessitating development alternative strategies for early detection effective medical intervention. Similar humans, canine age-related lifetime...
Background: Cardiotoxicity due to chemotherapy remains a challenge. Cardioprotective interventions have not slowed the progression heart failure. Cardiomyocyte (CM) death can be attributed autophagy impairment during chemotherapy-induced toxicity. We hypothesize that activation viable cardioprotective strategy. Methods: H9C2 rat cardiac myoblasts and slices generated from euthanized dog hearts were treated with 5-50μM doxorubicin (Dox) +/- activators for 24 hours. Annexin V (Anx) TUNEL...