A5059271878- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Molecular Junctions and Nanostructures
- RNA Interference and Gene Delivery
- Retinal Imaging and Analysis
- Quantum Dots Synthesis And Properties
- Connexins and lens biology
- Chalcogenide Semiconductor Thin Films
- Flavonoids in Medical Research
- Perovskite Materials and Applications
- Erythropoietin and Anemia Treatment
- Neurobiology and Insect Physiology Research
- Cancer-related molecular mechanisms research
- Insect Utilization and Effects
- Spinal Cord Injury Research
- Protein Hydrolysis and Bioactive Peptides
- Corneal surgery and disorders
- Peroxisome Proliferator-Activated Receptors
- Cancer, Hypoxia, and Metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Invertebrate Immune Response Mechanisms
- Enzyme Production and Characterization
- Retinal Development and Disorders
National Defense Medical Center
2019-2024
National Yang Ming Chiao Tung University
2024
Guangdong Academy of Medical Sciences
2022-2024
Southern Medical University
2024
Guangdong Provincial People's Hospital
2022-2024
Fujian Medical University
2018-2021
First Affiliated Hospital of Fujian Medical University
2018-2021
Chinese Academy of Sciences
2021
Simon Fraser University
2008-2017
Shenyang Pharmaceutical University
2016
In modern technology, optoelectronics plays a pivotal role, finding applications in diverse areas like solar cells, light-emitting diodes (LEDs), and photodetectors. Perovskite materials, known for their exceptional optical properties, are gaining attraction due to light weight, flexibility, ease of production. The expanding markets wearable electronics flexible displays have underscored the importance developing perovskite optoelectronic devices. However, prevalent use lead high-performance...
Flicking a switch: A robust DNA nanoswitch has been designed by using contractile construct (G-quadruplex) as the switch medium and ferrocene (Fc) redox-active indicator (see picture). The was electrochemically tested can be turned "on" or "off" repeatedly addition removal, respectively, of effector (K+ ions). reversibility repeatability are directly monitored electrochemical measurements.
The sensing principle of a new class DNA conformational switches (deoxyribosensors) is based on the incorporation an aptamer as receptor, whose altered conformation upon analyte binding conductivity adjacent helical conduction path, leading to increase in measured electrical signal through sensor. We report herein rational design and biochemical testing candidate deoxyribosensors for detection quantitation plasma protein, thrombin, followed by surface immobilization optimized sensor its...
Abstract Importance: Glaucoma is one of the world's leading causes irreversible blindness and effective interventions with less side effect cost were still in need. Objective: To investigate potential repurposing antihypertensive drugs to glaucoma treatment intra ocular pressure (IOP) lowering manners. Design, Setting Participants: Two sample Mendelian Randomization (MR) was used genetic on protection or IOP lowering. strategies (biomarker eQTL approach) applied define instrumental variable...
Double-helical DNA has been shown to conduct both electrons and electron holes, the latter over distances of >20 nm. is thus a material significant interest for bottom-up construction nanocircuitry. Here, we describe contractile nanoswitch, which can toggle between structurally extended "off" state contracted "on" state, with 40-fold conductivity difference two. To turn on, two short motifs guanine−guanine mismatches in an otherwise standard double helix synapse form conductive G-quadruplex,...
The development of high-performance Sn-based perovskite photodetectors is presented with double-sided passivation using large alkylammonium interlayers PEAI and BDAI₂. This dual strategy, applied to the top bottom FASnI₃ films, effectively improves film quality by reducing defect density, enhancing carrier mobility, minimizing non-radiative energy losses at interfaces. At 720 nm, demonstrate a responsivity 0.37 A W
The charge-flow properties and concomitant guanine damage patterns of a number intermolecular wholly parallel-stranded DNA G-quadruplexes were investigated. constructs structurally well-defined consisted the G-quadruplex sandwiched stacked between two Watson-Crick base-paired duplexes. Such duplex-quadruplex-duplex designed to minimize torsional stress as well steric crowding at duplex-quadruplex junctions. When anthraquinone was used induce charge flow within constructs, it found that...
Guanine-rich, single-stranded, DNAs and RNAs are able to fold form G-quadruplexes that held together by guanine base quartets. known bind ferric heme [Fe(III)-protoporphyrin IX] strongly activate such bound hemes toward peroxidase (1-electron oxidation) as well oxygenase/peroxygenase (2-electron activities. However, much remains unknown about how activation is effected. Herein, we investigated whether were strictly required for or related multi-stranded DNA/RNA structures isoguanine (iG)...
Purpose: The purpose of this study was to investigate the IOP-lowering effects ITRI-E-212, a new Rho-associated protein kinase (ROCK) inhibitor. ITRI-E-212 improved fluid outflow through trabecular meshwork and reduced IOP with transient mild conjunctival hyperemia. can potentially be developed into antiglaucoma agents. Methods: selected from more than 200 amino-isoquinoline structures because its adequate solubility drug-loading percentage in eye drops. has less 50% inhibitory concentration...
The Rho-associated protein kinase and myosin light chain (ROCK/MYLK) pathway undeniably plays a pivotal role in the pathophysiology of primary open-angle glaucoma (POAG). In our study, we utilized both ocular hypertension (OHT) rabbit models clinical investigations to gain invaluable insights that propel development novel treatments targeting proteins genes associated with trabecular meshwork (TM), thereby offering promising avenues for management POAG.
Ein robuster Nanoschalter beruht auf einem zusammenziehbaren DNA-Konstrukt (G-Quadruplex) als Schalter und Ferrocen (Fc) redoxaktivem Initiator (siehe Bild). Der wurde elektrochemisch getestet wiederholt durch Hinzufügen oder Entfernen des Effektors (K+-Ionen) an- ausgeschaltet. Reversibilität Reproduzierbarkeit DNA-Schalters können direkt elektrochemische Messungen verfolgt werden. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents...
Abstract Single‐stranded DNAs and RNAs that are rich in the nucleobase guanine form four‐stranded G‐quadruplexes, which held together by hydrogen‐bonded quartets. In aqueous solution, both DNA duplexes G‐quadruplexes modest conductors of electrical charge. A tight, topologically constrained construct called twDNA is now reported, a core four guanine‐rich single strands structurally electronically links double helices. The addition removal K + or Sr 2+ cations promote alternative conformers...
Currently, the role of Pannexin-1, a homomeric membrane hemichannel on neuron cell membrane, in development spinal cord injury (SCI) is largely unknown. Herein, we assessed contribution Panx1 SCI. The SCI vitro model was established using rat primary neurocytes treated with hydrogen peroxide (H 2 O ). Effects overexpression or depletion were analyzed by lentivirus-mediated transfection and interference sh-Panx1. Decreased viability seen cells, which further enhanced under mitigated...
The aim of this study was to investigate whether exogenous erythropoietin (EPO) administration attenuates N-methyl-D-aspartate (NMDA)-mediated excitotoxic retinal damage in Wistar rats. survival rate ganglion cells (RGCs) were investigated by flat mount analysis and flow cytometry. A total 125 male rats randomly assigned five groups: negative control, NMDA80 (i.e., 80 nmoles NMDA intravitreally injected), + 10ng EPO, 50ng 250ng EPO. EPO treatment group used evaluate various administrated...
Purpose: The purpose of this study was to develop a preclinical compound, ITRI-E-(S)4046, dual synergistic inhibitor myosin light chain kinase 4 (MYLK4) and Rho-related protein (ROCK), for reducing intraocular pressure (IOP). Methods: ITRI-E-(S)4046 is an amino-pyrazole derivative with physical chemical properties suitable ophthalmic formulation. In vitro inhibition evaluated using the Kinase-Glo Luminescent Kinase Assays. A comprehensive selectivity analysis performed KINOMEscan assay from...
Abstract Single‐stranded DNAs and RNAs that are rich in the nucleobase guanine form four‐stranded G‐quadruplexes, which held together by hydrogen‐bonded quartets. In aqueous solution, both DNA duplexes G‐quadruplexes modest conductors of electrical charge. A tight, topologically constrained construct called twDNA is now reported, a core four guanine‐rich single strands structurally electronically links double helices. The addition removal K + or Sr 2+ cations promote alternative conformers...
Objective Glaucoma is an optic neuropathy and the leading cause of irreversible blindness worldwide. However, early detection glaucoma remains challenging, as chronic forms remain largely asymptomatic until considerable visual field deficits have ensued. Thus, biomarkers that facilitate diagnosis treatment for patients with a high risk progression are pressing. Methods analysis Human disease–biomarker interactions network human disease–target–drug were first constructed based on multiomics...
Objective To investigate the expression changes and repair effect of mitogen stress- activated protein kinase 1 (MSK1) on spinal cord injury (SCI) in rats. Methods One hundred twenty male Sprague Dawley (SD) rats (weighing 220-250 g) were used for study, 70 them randomly divided into sham-operation group SCI ( n=35), given according to Allen's method, only opened lamina without injuring cord; tissue was collected at 8 hours, 12 day, 2 days, 3 5 7 days after invasive treatment, each detect...
DNAs and RNAs that fold via the formation of guanine quartets form G-quadruplexes are often highly diverse in terms architecture topology. specifically stabilized by metal cations such as K(+) Sr(2+), but not Li(+). DNA duplexes incorporate two separated clusters G•G mismatches ("P-duplexes") can function electronic switches, capable toggling reversibly from a poorly conductive conformer (E) with only Li(+) solution to G-quadruplex incorporating higher conductivity (C) presence K(+). Herein,...
Abstract Purpose Glaucoma is an optic neuropathy, and the leading cause of irreversible blindness worldwide. However, early detection glaucoma remains challenging as chronic forms remain largely asymptomatic until considerable visual field deficits have ensued. Thus, biomarkers that facilitate diagnosis treatment for patients with a high risk progression are pressing. Methods Human disease-biomarker interactions network (HDBIN) disease-target-drug (HDTDIN) were first constructed based on...