A5082487959- Axon Guidance and Neuronal Signaling
- Gut microbiota and health
- Angiogenesis and VEGF in Cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Nerve injury and regeneration
- Cancer, Hypoxia, and Metabolism
- Parkinson's Disease Mechanisms and Treatments
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- interferon and immune responses
- Diet and metabolism studies
- Tryptophan and brain disorders
- Autophagy in Disease and Therapy
- Cancer Immunotherapy and Biomarkers
- Psoriasis: Treatment and Pathogenesis
- Cancer Cells and Metastasis
- Genetic factors in colorectal cancer
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Zebrafish Biomedical Research Applications
- Metabolomics and Mass Spectrometry Studies
- Adenosine and Purinergic Signaling
- Colorectal Cancer Treatments and Studies
- Inflammasome and immune disorders
- IL-33, ST2, and ILC Pathways
University of Luxembourg
2015-2025
Inserm
2006-2010
Neuropsychologie Cognitive et Physiopathologie de la Schizophrénie
2009-2010
Neuroinflammation is the local reaction of brain to infection, trauma, toxic molecules or protein aggregates. The resident macrophages, microglia, are able trigger an appropriate response involving secretion cytokines and chemokines, resulting in activation astrocytes recruitment peripheral immune cells. IL-1β plays important role this response; yet its production mode action not fully understood precise implication neurodegenerative diseases needs further characterization. Our results...
Abstract The gut microbiome is a key player in the immunomodulatory and protumorigenic microenvironment during colorectal cancer (CRC), as different gut-derived bacteria can induce tumour growth. However, crosstalk between host relation to cell metabolism remains largely unexplored. Here we show that formate, metabolite produced by CRC-associated bacterium Fusobacterium nucleatum , promotes CRC development. We describe molecular signatures linking phenotypes with abundance. Cocultures of F....
In solid tumors, cancer stem cells (CSCs) or tumor-initiating (TICs) are often found in hypoxic niches. Nevertheless, the influence of hypoxia on TICs is poorly understood. Using previously established, TIC-enrichedpatient-derived colorectal (CRC) cultures, we show that increases self-renewal capacity while inducing proliferation arrest their more differentiated counterpart cultures. Gene expression data revealed macroautophagy/autophagy as one major pathways induced by TICs. Interestingly,...
Colorectal cancer (CRC) patients have been shown to possess an altered gut microbiome. Diet is a well-established modulator of the microbiome, and thus, dietary interventions might beneficial effect on CRC. An attenuating ketogenic diet (KD) CRC cell growth has previously observed, however role microbiome in driving this remains unknown. Here, we describe reduced colonic tumor burden upon KD consumption mouse model with humanized Importantly, demonstrate causal relationship through...
Abstract A key pathological process in Parkinson's disease (PD) is the transneuronal spreading of α‐synuclein. Alpha‐synuclein (α‐syn) a presynaptic protein that, PD, forms inclusions. Other hallmarks PD include neurodegeneration and microgliosis susceptible brain regions. Whether it primarily α‐syn particles, inclusion formation, or other mechanisms, such as inflammation, that cause unclear. We used model induced by striatal injection preformed fibrils into mouse striatum to address this...
Abstract Fibroblasts have a considerable functional and molecular heterogeneity can play various roles in the tumor microenvironment. Here we identify pro-tumorigenic IL1R1 + , IL-1-high-signaling subtype of fibroblasts, using multiple colorectal cancer (CRC) patient single cell sequencing datasets. This fibroblasts is linked to T macrophage suppression leads increased growth 3D co-culture assays. Furthermore, both fibroblast-specific knockout IL-1 receptor antagonist Anakinra administration...
saves mutant mice from infection-induced lethality
Abstract Metastasis is the most common cause of death in cancer patients. Canonical drugs target mainly proliferative capacity cells, which leaves slow-proliferating, persistent cells unaffected. Metabolic determinants that contribute to growth-independent functions are still poorly understood. Here we show antifolate treatment results an uncoupled and autarkic mitochondrial one-carbon (1C) metabolism during cytosolic 1C impairment. Interestingly, dependent growth-arrest does not correlate...
Inflammatory bowel diseases are characterized by the chronic relapsing inflammation of gastrointestinal tract. While molecular causality between endoplasmic reticulum (ER) stress and intestinal is widely accepted, metabolic consequences ER on pathophysiology IBD remain unclear. By using in vitro, vivo models, patient datasets, we identified a distinct polarization mitochondrial one-carbon metabolism fine-tuning amino acid uptake epithelial cells tailored to support GSH NADPH upon stress....
Brain inflammation, a common feature in neurodegenerative diseases, is complex series of events, which can be detrimental and even lead to neuronal death. Nonetheless, several studies suggest that inflammatory signals are also positively influencing neural cell proliferation, survival, migration, differentiation. Recently, correlative suggested astrocytes able dedifferentiate upon injury may thereby re-acquire stem (NSC) potential. However, the mechanism underlying this dedifferentiation...
Blood analysis is one of the foundations clinical diagnostics. In recent years, proteins in blood samples by mass spectrometry has taken a jump forward terms sensitivity and number identified proteins. The development parallel reaction monitoring with accumulation serial fragmentation (prm-PASEF) combines ion mobility as an additional separation dimension. This increases proteome coverage while allowing use shorter chromatographic gradients. To demonstrate method's full potential, we used...
Abstract Background Inflammatory Bowel Disease (IBD) is characterized by chronic inflammation and metabolic dysregulation in the intestinal epithelium. Serine, a non-essential amino acid synthesized via rate-limiting enzyme phosphoglycerate dehydrogenase (PHGDH) maintains cellular redox balance. Serine metabolism upregulated cancer immune cells, supporting survival growth. Our previous story shows ER stress-mediated rewiring of serine key molecular feature IBD. However, mechanism how it...
Semaphorin 3A (Sema3A) is a secreted guidance molecule initially described in the nervous system. This protein able to control axon growth but also effects on endothelial cells migration. Here, we report that Sema3A acts as chemorepellent factor for rat C6 glioma and 3 different human cell lines. Interestingly, triggered chemoattractive response fourth line. The nature of receptor complex ensuring appropriate signaling was dissected by using function blocking antibodies gain- or loss-of...
There is increasing evidence for a crucial role of proteases and metalloproteinases during axon growth guidance. In this context, we recently described functional link between the chemoattractive Sema3C Matrix metalloproteinase 3 (MMP3). Here, provide data demonstrating involvement MMP-2 to trigger growth-promoting effect Sema3A in cortical dendrites. The situ analysis expression activity consistent with assay vitro that pharmacological inhibition reduces dendrites response Sema3A. Hence,...
Abstract Neural representations of the environment within brain take form topographic maps whose formation relies on graded expression axon guidance molecules. Retinocollicular map formation, from retinal ganglion cells (RGCs) to superior colliculus (SC) in midbrain, is mainly driven by Eph receptors and their ligands ephrins. However, other molecules participate this map. Here we demonstrate that receptor Neuropilin‐2 expressed an increasing nasal–temporal gradient RGCs, whereas one its...
Although the intestinal tract is a major site of reactive oxygen species (ROS) generation, mechanisms by which antioxidant defense in gut T cells contribute to homeostasis are currently unknown. Here we show, using cell-specific ablation catalytic subunit glutamate cysteine ligase (
Inflammatory bowel disease (IBD) is associated with perturbed metabolism of the essential amino acid tryptophan (Trp). Whether increased degradation Trp directly fuels mucosal inflammation or acts as a compensatory attempt to restore cellular energy levels via
Astrocytes, the most abundant glial cell population in central nervous system, have important functional roles brain as blood barrier maintenance, synaptic transmission or intercellular communications [1], [2]. Numerous studies suggested that astrocytes exhibit a and morphological high degree of plasticity. For example, following any injury, become reactive hypertrophic. This phenomenon, also called gliosis, is characterized by set progressive gene expression cellular changes [3]....
The chiral molecule, apomorphine, is currently used for the treatment of Parkinson's disease (PD). As a potent dopamine receptor agonist, this lipophilic compound especially effective treating motor fluctuations in advanced PD patients. In addition to its receptor-mediated actions, apomorphine has also antioxidant and free radical scavenger activities. Neuroinflammation, oxidative stress, microglia reactivity have emerged as central players PD. Thus, modulating activation may be valid...
Abstract Background Inflammatory bowel disease is characterized by disturbed metabolism, including disrupted tryptophan (TRP) metabolism along the kynurenine pathway (KP). However, molecular mechanism how this fuels inflammation not understood. Here, we unravel rewiring of TRP in IBD combining murine DSS colitis with multi-omics longitudinal cohorts. Methods Serum metabolites were associated CRP, e/pMayo, SESCD and CDAI (n=83 UC, n=51 CD). Mucosal IDO1 QPRT expression (both KP enzymes;...