A5056595640- Cancer Genomics and Diagnostics
- Immune Cell Function and Interaction
- Photosynthetic Processes and Mechanisms
- T-cell and B-cell Immunology
- Light effects on plants
- Immunotherapy and Immune Responses
- Photoreceptor and optogenetics research
- Lung Cancer Treatments and Mutations
- RNA Interference and Gene Delivery
- Breast Cancer Treatment Studies
- Cellular transport and secretion
- Epigenetics and DNA Methylation
- Gut microbiota and health
- Immune Response and Inflammation
- Complement system in diseases
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Pancreatic and Hepatic Oncology Research
- Leech Biology and Applications
- PARP inhibition in cancer therapy
- Extracellular vesicles in disease
- Advanced Breast Cancer Therapies
- Breast Lesions and Carcinomas
- Bacterial biofilms and quorum sensing
- Cancer Immunotherapy and Biomarkers
- Listeria monocytogenes in Food Safety
Avera Health
2010-2023
Avera McKennan Hospital & University Health Center
2017-2023
University of South Dakota
1999-2020
Sioux Falls VA Health Care System
2010-2020
University of Michigan
2008-2017
University of Sioux Falls
2010
University of Nebraska Medical Center
2003-2006
University of Iowa
2006
The Netherlands Cancer Institute
1998
The ability to perform comprehensive profiling of cancers at high resolution is essential for precision medicine. Liquid biopsies using shed exosomes provide high-quality nucleic acids obtain molecular characterization, which may be especially useful visceral that are not amenable routine biopsies.We isolated in biofluids from three patients with pancreaticobiliary (two pancreatic, one ampullary). We performed exoDNA and exoRNA by whole genome, exome transcriptome sequencing the Illumina...
Abstract Tapasin influences the quantity and quality of MHC/peptide complexes at cell surface; however, little is understood about structural features that underlie its effects. Because tapasin, MHC class I, TAP are transmembrane proteins, tapasin transmembrane/cytoplasmic region has potential to affect interactions endoplasmic reticulum membrane. In this study, we have assessed influence a conserved lysine position 408, which lies in domain. We found substitutions K408 affected expression I...
Despite the urgency for prevention and treatment of lung adenocarcinoma (LUAD), we still do not know drivers in pathogenesis disease. Earlier work revealed that mice with knockout G-protein coupled receptor Gprc5a develop late onset tumors including LUADs. Here, sought to further probe impact expression on LUAD pathogenesis. We first surveyed GPRC5A human tissues found was markedly elevated normal relative other consistently downregulated In sharp contrast wild-type littermates,
Telomere length has garnered interest due to the potential role it may play as a biomarker for cellular aging process. measurements obtained from blood-derived DNA are often used in epidemiological studies. However, invasive nature of blood draws severely limits sample collection, particularly with children. Buccal cells commonly sampled isolation and thus present non-invasive alternative telomere measurement. leukocyte derived samples collected at same time period were analyzed repeat mass...
Abstract Presentation of antigenic peptides to T lymphocytes by MHC class I molecules is regulated events involving multiple endoplasmic reticulum proteins, including tapasin. By studying the effects substitutions in tapasin Ig-like domain, we demonstrated that H-2Ld/tapasin association can be segregated from reconstitution folded Ld surface expression. This finding suggests peptide acquisition influenced functions are independent binding. We also found presence a nine-amino acid region...
Phosphorylated phosphoinositide lipids (PPIs) are low-abundance signaling molecules that control signal transduction pathways and necessary for cellular homeostasis. The PPI phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P 2 ) is essential in multiple organ systems. PI(3,5)P generated from PI3P by the conserved lipid kinase Fab1/PIKfyve. Defects dynamic regulation of linked to human diseases. However, few mechanisms regulate have been identified. Here we report an intramolecular interaction...
Microbial dysbiosis has emerged as a modulator of oncogenesis and response to therapy, particularly in lung cancer. Here, we investigate the evolution gut microbiomes following exposure tobacco carcinogen. We performed 16S rRNA-Seq fecal samples collected prior at several timepoints (nicotine-specific nitrosamine ketone/NNK)
DNA photolyases catalyze the blue light-dependent repair of UV light-induced damage in DNA. are specific for either cyclobutane-type pyrimidine dimers or (6–4) photoproducts. PHR2 is a gene that Chlamydomonas reinhardtii encodes class II photolyase which catalyzes photorepair dimers. Based on amino acid sequence analysis PHR2, indicates presence chloroplast targeting sequence, was predicted to encode Chlamydomonas. Using sensitive gene-specific vivo assay, we found overexpression results...
Lung cancer is the leading cause of cancer-related deaths worldwide. Surgery and chemoradiation are standard care in early stages non-small cell lung (NSCLC), while immunotherapy late-stage NSCLC. The immune composition tumor microenvironment (TME) recognized as an indicator for responsiveness to immunotherapy, although much remains unknown about its role surgery or chemoradiation. In this pilot study, we characterized NSCLC TME using mass cytometry (CyTOF) bulk RNA sequencing (RNA-Seq) with...
A protein of unknown physiological function, called amyloid precursor-like 2 (APLP2), forms an association with the murine class I molecule Kd that is up-regulated by presence adenoviral E3/19K. We have extended these findings to show APLP2 and E3/19K associate preferentially folded not open form. was detectable at cell surface, but its surface expression concurrent Kd. Experimental down-regulation caused a consistent increase in Kd, indicating normally reduces expression. These data suggest...
Abstract Background: Emerging research underscores the gut microbiome's critical role in cancer development, progression, and response to treatment. As a part of “ASAP” study, which focuses on implementation comprehensive molecular profiling deep clinical annotation electronic health records participants diagnosed with or at risk developing cancer, we have established microbiome-associated biobank corresponding dataset systematically explore dynamics microbiome as patients progress through...
Carbamoylated erythropoietin (CEPO) is a chemically engineered, nonhematopoietic derivative of (EPO) that retains its antidepressant and pro-cognitive effects, which are attributed to the increased expression neurotrophic factors like brain derived factor (BDNF), in central nervous system. However, chemical modification process produces CEPO from requires pure EPO as raw material, challenging scale-up can also cause batch-to-batch variability. To address these key limitations while retaining...
Abstract The medicinal leech, Hirudo verbana , is a powerful model organism for investigating fundamental neurobehavioral processes. well-documented arrangement and properties of H. ’s nervous system allows changes at the level specific neurons or synapses to be linked physiological behavioral phenomena. Juxtaposed extensive knowledge verbana’s limited, but recently expanding, portfolio molecular multi-omics tools. Together, advancement genetic databases will complement existing...
The overall 5-year survival of lung cancer remains dismal despite the current treatment regimens. Testing for driver mutations has become routine practice oncologists due to presence targeted therapy readily available patients. Deep sequencing through next generation (NGS) is an adequate methodology detect at multi-genetic levels. molecular pathology non-small cell (NSCLC) poorly understood in Middle East and, date, no other reports have been published on deep adenocarcinoma (LUAD)...
Metastatic breast cancer is one of the leading causes cancer-related death in women. Limited studies have been done on genomic evolution between primary and metastatic cancer. We reconstructed through 16-yr history an ER + HER2 − patient to investigate molecular mechanisms disease relapse treatment resistance after long-term exposure hormonal therapy. Genomic transcriptome profiling was performed tumor (2002), initial recurrence (2012), liver metastasis (2015) samples. Cell-free DNA analysis...