A5007702815- Advanced biosensing and bioanalysis techniques
- Quantum Dots Synthesis And Properties
- Bacillus and Francisella bacterial research
- Microbial Community Ecology and Physiology
- Astro and Planetary Science
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Isotope Analysis in Ecology
- Planetary Science and Exploration
- Gene expression and cancer classification
- Health, Environment, Cognitive Aging
- RNA Interference and Gene Delivery
- Bacterial Genetics and Biotechnology
- Advanced Biosensing Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Health and Medical Research Impacts
- SARS-CoV-2 and COVID-19 Research
- Scientific Computing and Data Management
- Nanocluster Synthesis and Applications
- Hepatitis B Virus Studies
- Chalcogenide Semiconductor Thin Films
- Genetic and phenotypic traits in livestock
- Science, Research, and Medicine
- COVID-19 Clinical Research Studies
- Viral Infections and Outbreaks Research
George Mason University
2001-2024
Johns Hopkins University Applied Physics Laboratory
2011-2023
Johns Hopkins University
2011-2023
Center for Environmental Health
2017
Defense Health Agency
2017
United States Naval Research Laboratory
2007-2014
Naval Research Laboratory Center for Bio/Molecular Science and Engineering
2008-2013
Center for Molecular Medicine and Immunology
2013
K Lab (United States)
2009-2010
Weatherford College
2009
Abstract Enceladus’s long-lived plume of ice grains and water vapor makes accessing oceanic material readily achievable from orbit (around Saturn or Enceladus) the moon’s surface. In preparation for National Academies Sciences, Engineering Medicine 2023–2032 Planetary Science Astrobiology Decadal Survey, we investigated four architectures capable collecting analyzing and/or on surface to address most pressing questions at Enceladus: Is subsurface ocean inhabited? Why, why not? Trades...
Iron deposits (Fe plaque) on wetland plant roots contain abundant microbial populations, including Fe(II)-oxidizing bacteria (FeOB) that have not been cultured previously. In this study, 4 strains of Fe plaque-associated FeOB were isolated from species plants. All isolates grew in tight association with Fe-oxides, but did form any identifiable Fe-oxide structures. obligate lithotrophic Fe(II)-oxidizers microaerobic, and unable to use other inorganic or organic energy sources. One strain,...
Journal Article Delivering quantum dot-peptide bioconjugates to the cellular cytosol: escaping from endolysosomal system Get access James B. Delehanty, Delehanty Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory, Washington DC 20375, USA E-mail: James.delehanty@nrl.navy.mil Search other works by this author on: Oxford Academic Google Scholar Christopher E. Bradburne, Bradburne Kelly Boeneman, Boeneman Kimihiro Susumu, Susumu Optical Sciences...
Interest in taking advantage of the unique spectral properties semiconductor quantum dots (QDs) has driven their widespread use biological applications such as vitro cellular labeling/imaging and sensing. Despite demonstrated utility, concerns over potential toxic effects QD core materials on proliferation homeostasis have persisted, leaving question suitability QDs alternatives for more traditional fluorescent (e.g., organic dyes, proteins) applications. Surprisingly, direct comparative...
Multicolor fluorescent labeling of both intra- and extracellular structures is a powerful technique for simultaneous monitoring multiple complex biochemical processes. This approach remains extremely challenging, however, as it often necessitates the combinatorial use numerous targeting probes (e.g., antibodies), multistep bioconjugation chemistries, different delivery strategies electroporation or transfection reagents), cellular fixation coupled with membrane permeabilization, spectral...
Biological agents including viruses, bacteria, and other naturally occurring pathogenic organisms, along with the toxins they produce, are considered far harder to detect defend against than chemical agents. Here we provide an overview of predominant molecular sensing technologies for detection these This includes biosensing strategies based upon use antibodies, genomic analysis, biochemical testing, recognition interactions, cellular-based responses. We survey some popular approaches,...
Abstract Background Monoclonal antibodies (mAbs) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are a promising treatment for limiting the progression of disease 2019 (COVID-19) and decreasing strain on hospitals. Their use, however, remains limited, particularly in disadvantaged populations. Methods Electronic health records were reviewed from SARS-CoV-2 patients at single medical center United States that initiated mAb infusions January 2021 with support US Department...
Systemic pro-inflammatory cytokine release has been previously implicated as a major death-causing factor in anthrax, however, direct data have absent. We determined the levels of IL-1 beta, IL-6 and TNF-alpha serum mice challenged with virulent (Ames) or attenuated (Sterne) strains Bacillus anthracis. More than 10-fold increase IL-1beta was detected Ames-challenged Balb/c mice, contrast to more susceptible C57BL/6 which showed no response. also responded higher IL-6. The A/J demonstrated...
Abstract Background The state-of-the-art in nucleic acid based biodetection continues to be polymerase chain reaction (PCR), and many real-time PCR assays targeting biodefense pathogens for biosurveillance are widespread use. These predominantly singleplex; i.e. one assay tests the presence of target, found a single organism, sample at time. Due intrinsic limitations such tests, there exists critical need high-throughput multiplex reduce time cost incurred when screening multiple targets,...
Carboxysomes, microcompartments that enhance the fixation of carbon dioxide by Rubisco, are found in several chemoautotrophs and all cyanobacteria thus far examined. The genes for Rubisco large (cbbL) small (cbbS) subunits (cbb Calvin-Benson-Bassham), along with (csoS) carboxysome shell peptides, organized a putative operon Halothiobacillus neapolitanus following order: cbbL,cbbS, csoS2, csoS3, orfA, orfB, csoS1C, csoS1A, csoS1B. DNA sequencing has revealed essentially same three other...
Carboxysomes containing the Calvin cycle enzyme ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) have been demonstrated in a variety of chemoautotrophic prokaryotes and cyanobacteria. The genes ccm cso operon Synechococcus sp. PCC7942 Thiobacillus neapolitanus, respectively, code for several carboxysome polypeptides. polypeptides CcmK CsoS1 exhibit high degree conservation, turn show significant homology to CchA PduA ethanolamine propanediol operons enteric bacteria. Probing...
Abstract Background It has been recently reported that major pathogens Staphylococcus aureus and Pseudomonas aeruginosa accelerate a normal process of cell surface syndecan-1 (Synd1) ectodomain shedding as mechanism host damage due to the production shedding-inducing virulence factors. We tested if acceleration Synd1 takes place in vitro upon treatment epithelial cells with B. anthracis hemolysins, well vivo during anthrax infection mice. Results The isolated hemolytic proteins AnlB...
Modular peptides displaying both quantum dot bioconjugation motifs and specific subcellular targeting domains were constructed using a chemoselective aniline-catalyzed hydrazone coupling chemistry. Peptides ratiometrically assembled onto dots to facilitate their delivery either the plasma membrane, endosomes, cytosol or mitochondria of target cells.
Pneumonic tularemia is caused by inhalation of Francisella tularensis, one the most infectious microbes known. We wanted to study kinetics initial and early interactions between bacterium host cells in lung. To do this, we examined infection A549 airway epithelial with live vaccine strain (LVS) F. tularensis. were infected analyzed for global transcriptional response at multiple time points up 16 h following infection. At 15 min 2 h, a strong was observed including cytoskeletal...
Reference genome fidelity is critically important for wide association studies, yet most vary widely from the study population. A typical whole sequencing approach implies short-read technologies resulting in fragmented assemblies with regions of ambiguity. Further information lost by economic necessity when genotyping populations, as lower resolution such arrays are commonly used. Here, we present a phased reference Canis lupus familiaris using high molecular weight DNA-sequencing...
Bacillus anthracis secretes several virulence factors targeting different host organs and cell types during inhalational anthrax infection. The bacterial expression of a key factor, lethal toxin (LeTx) is closely tied to another edema (EdTx). Both are transcribed on the same plasmid (pXO1) both have been subject much individual study. Their combined effect virulent likely modulates global transcriptional phenotypic response macrophages phagocytes. In fact, responses brought about by toxins...
Abstract Estimates of 7x105 cubic meters crude oil were released into the Gulf Mexico as a consequence April 20th, 2010 Deep Water Horizon drilling rig explosion, leaving thousands square miles earth's surface covered in oil. Dispersants used on large slicks and injected at well head, resulting being suspended throughout water column. Starting June 2010, reached hundreds Louisiana, Alabama, Mississippi, Florida shoreline disturbing ecological balance economic stability region. While...