Abstract The native extracellular matrix (ECM) generally exhibits dynamic mechanical properties and displays time‐dependent responses to deformation or loading, in terms of viscoelastic behaviors (e.g., stress relaxation creep). Viscoelasticity the ECM plays a critical role development, homeostasis, tissue regeneration, its implication disease progression has also been recognized recently. Hydrogels with tunable hold great promise recapitulate such mechanics found ECM, which have recently...

Abstract Atopic dermatitis (AD) is a chronic skin disease caused by immune dyshomeostasis and accompanied severe pruritus. Although oxidative stress mechanical scratching can aggravate AD inflammation, treatment targeting often overlooked, the efficiency of mechano-chemically synergistic therapy remains unclear. Here, we find that enhanced phosphorylation focal adhesion kinase (FAK) associated with scratch-exacerbated AD. We then develop multifunctional hydrogel dressing integrates...

Postoperative adhesions occur widely in various tissues, bringing the risk of secondary surgery and increased medical burden. Hydrogel barriers with Janus-adhesive ability can achieve physical isolation adjacent tissues are therefore considered an ideal solution. However, integrating endoscopic delivery convenience viscoelastic Janus hydrogel formation remains a great challenge. Here, we present report situ barrier using sprayable fast-Janus-gelation (FJG) powder. We first methacrylate...

Brain lesions can arise from traumatic brain injury, infection, and craniotomy. Although injectable hydrogels show promise for promoting healing of health surrounding tissue, enabling cellular ingrowth restoring neural tissue continue to be challenging. It is hypothesized that these challenges in part the mismatch composition, stiffness, viscoelasticity between hydrogel parenchyma, this hypothesis tested by developing evaluating a self-healing not only mimics but also stiffness native...

The endothelial cell (EC) outgrowth in both vasculogenesis and angiogenesis starts with remodeling surrounding matrix proceeds the crosstalk between cells for multicellular vasculature formation. mechanical plasticity of matrix, defined as ability to permanently deform by external traction, is pivotal modulating behaviors. Nevertheless, implications on cell-to-cell interactions during EC outgrowth, along molecular pathways involved, remain elusive. Here we develop a collagen-hyaluronic acid...

Abstract Injury to the central nervous system (CNS) usually leads activation of astrocytes, followed by glial scar formation. The formation scars from active astrocytes in vivo has been found be dependent on cell microenvironment. However, how respond different microenvironmental cues during formation, such as changes matrix stiffness, remains elusive. In this work, we established an vitro model assess responses stiffness that may related pathophysiology. investigated hydrogel backbones are...

Periodontitis can cause irreversible defects in the periodontal ligament (PDL), regeneration of which is major obstacle to clinical treatment periodontitis. Implanting hydrogel for releasing anti-inflammatory drugs a promising promote PDL regeneration. However, existing systems fail mimic typical viscoelastic feature native periodontium, may have been shown as an important role tissue Meanwhile, synergistic benefits mechanical cues and biochemical agents remain elusive. In this study, we...

Three-dimensional (3D) bioprinting has become a promising approach to construct­ing functional biomimetic tissues for tissue engineering and regenerative medicine. In 3D bioprinting, bio-inks are essential the construction of cell microenviron­ment, thus affecting design efficiency. Mechanical properties one aspects microenvironment, which can be char­acterized by matrix stiffness, viscoelasticity, topography, dynamic mechanical stimulation. With recent advances in...

Abstract Cardiac fibrosis is a common pathway leading to heart failure and involves continued activation of cardiac fibroblasts (CFs) into myofibroblasts during myocardium damage, causing excessive deposition the extracellular matrix (ECM) thus increases stiffness. Increasing evidence has shown that stiffened plays an important role in promoting CF fibrosis, several signaling factors mediating mechanotransduction have been identified. However, key molecules perceive stiffness regulate remain...

Abstract Cell shape plays important roles in regulating cell behavior; however, independently controlling three dimensions is a challenging undertaking, and how affects cellular responses to mechanical biochemical cues remains unclear. Here, we present hydrogel-based platform control by using sequentially formed hybrid hydrogels consisting of collagen alginate. By adjusting the cross-linking time alginate, fixed NIH 3T3 fibroblasts at different spreading states. Then, explored influence on...

Chromium carbide nanopowders were synthesized by mechanical alloying-assisted microwave heating. The effect of gamma irradiation on phase composition and microstructure chromium the heating method was analyzed. samples characterized X-ray diffractometry (XRD), photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), transmission (TEM), high-resolution (HRTEM) techniques. results showed that well-dispersed can be maintaining temperature at 1000 °C for 1 h. Gamma ray had an...

Abstract Natural extracellular matrix (ECM) mostly has a fibrous structure that supports and mechanically interacts with local residing cells to guide their behaviors. The effect of ECM elasticity on cell behaviors been extensively investigated, while less attention paid the fiber‐network plasticity at microscale, although plastic remodeling is common phenomenon in fibrosis. Here, significant decrease found native fibrotic tissues, which associated an increase crosslinking. To explore role...

The fiber structures of tumor microenvironment (TME) are well-known in regulating cell behaviors, and the plastic remolding TME has recently been suggested to enhance metastasis as well. However, interrelationship between microarchitecture matrix plasticity is inextricable by existing vitro models. individual roles tuning behaviors remain elusive. This study develops an interpenetrating collagen-alginate hydrogel platform with independently tunable through strategy alginate networks collagen...

After myocardial infarction (MI), ventricular dilation and the microscopic passive stretching of infarcted border zone is meaning contributor to continuous expansion fibrosis. Epicardial hydrogel patches have been demonstrated alleviate this sequela MI in small-animal models. However, these not successfully translated humans or even large animals, part because challenges attaining both greater stiffness slower viscoelastic relaxation that mathematical models predict be optimal for...

Abstract The endothelial cell (EC) outgrowth in both vasculogenesis and angiogenesis starts with remodeling surrounding matrix proceeds the crosstalk between cells for multicelluar vasculature formation. Althought mechanical plasticity of has been recognized to regulate individual behaviors through plastic remodeling, it remains elusive how impacts cell-to-cell interplay during EC underlying molecular pathways. Here we developed a collagen-hyaluronic acid based hydrogel platform tunable...

Abstract Brain lesions can arise from traumatic brain injury, infection, and craniotomy. Although injectable hydrogels show promise for promoting healing of health surrounding tissue, enabling cellular ingrowth restoring neural tissue continue to be challenging. We hypothesized that these challenges in part viscoelastic mismatch between the hydrogel parenchyma, tested this hypothesis by developing evaluating a self-healing mimicked both composition viscoelasticity native parenchyma. The was...

Visualizing cellular structures especially the cytoskeleton and nucleus is crucial for understanding mechanobiology, but traditional fluorescence staining has inherent limitations such as phototoxicity photobleaching. Virtual techniques provide an alternative approach to addressing these issues often require substantial amount of user training data. In this study, we develop a generalizable cell virtual toolbox (termed CellVisioner) based on few-shot transfer learning that requires...