A5015696435- Poxvirus research and outbreaks
- Bacteriophages and microbial interactions
- Herpesvirus Infections and Treatments
- Bacterial Infections and Vaccines
- Parasitic Infections and Diagnostics
- Pneumonia and Respiratory Infections
- Infective Endocarditis Diagnosis and Management
- Legionella and Acanthamoeba research
- Advanced biosensing and bioanalysis techniques
- Bacillus and Francisella bacterial research
- Chemical Synthesis and Analysis
- HIV/AIDS drug development and treatment
- Aquaculture disease management and microbiota
- Chemical Synthesis and Reactions
- Enterobacteriaceae and Cronobacter Research
- Diphtheria, Corynebacterium, and Tetanus
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
- Viral gastroenteritis research and epidemiology
- Oxidative Organic Chemistry Reactions
- Click Chemistry and Applications
- Antifungal resistance and susceptibility
- Genomics and Phylogenetic Studies
- Congenital Anomalies and Fetal Surgery
- Parasitic infections in humans and animals
University of Guelph
2024-2025
Centers for Disease Control and Prevention
2006-2018
National Center for Emerging and Zoonotic Infectious Diseases
2013-2016
Public Health Agency of Canada
2011
National Health Laboratory Service
2011
National Institute for Communicable Diseases
2011
Influenza A virus reservoirs in animals have provided novel genetic elements leading to the emergence of global pandemics humans. Most influenza viruses circulate waterfowl, but those that infect mammalian hosts are thought pose greatest risk for zoonotic spread humans and generation pandemic or panzootic viruses. We identified an from little yellow-shouldered bats captured at two locations Guatemala. It is significantly divergent known The HA bat was estimated diverged roughly same time as...
Cholera was absent from the island of Hispaniola at least a century before an outbreak that began in Haiti fall 2010. Pulsed-field gel electrophoresis (PFGE) analysis clinical isolates and recent global travelers returning to United States showed indistinguishable PFGE fingerprints. To better explore genetic ancestry strain, we acquired 23 whole-genome Vibrio cholerae sequences: 9 obtained or Dominican Republic, 12 pattern-matched linked Asia Africa, 2 nonmatched outliers Western Hemisphere....
Cowpox virus (CPXV) is described as the source of first vaccine used to prevent onset and spread an infectious disease. It one earliest members genus Orthopoxvirus, which includes viruses that cause smallpox monkeypox in humans. Both historic current literature describe "cowpox" a disease with single etiologic agent. Genotypic data presented herein indicate CPXV not species, but composite several (up 5) species can infect cows, humans, other animals. The practice naming agents after host...
During 2010 and 2012, California Vermont, respectively, experienced statewide epidemics of pertussis with differences seen in the demographic affected, case clinical presentation, molecular epidemiology circulating strains. To overcome limitations current typing methods for pertussis, we utilized whole-genome sequencing to gain a broader understanding how strains are causing large epidemics. Through use combined next-generation technologies, this study compared de novo, single-contig genome...
In article 10.1002/cbic.202400643, Derek K. O′Flaherty and co-workers report an aqueous-compatible conjugation strategy for nucleic acids that uses amino modifiers to synthesize phosphoramidate- or amide-containing sequences while bound a solid support. This approach successfully incorporates range of ligand handles, such as azides metal chelators. The method provides simple, efficient, robust way conjugate desirable ligands under mild aqueous conditions. cover feature illustrates the...
Amino‐modifiers are pivotal chemical modifications in nucleic acid scaffolds, serving applications ranging from (bio)conjugation to probing the origins of life. We report a simple, efficient, and cost‐effective methodology for introduction amino‐modifiers into DNA RNA. This approach leverages commercially available sulfonyl‐containing solid support, which is first converted mixed N‐hydroxysuccinimide carbonate, enabling robust conjugation with primary secondary amines whether nucleosidic or...
ABSTRACT Recent studies of varicella-zoster virus (VZV) DNA sequence variation, involving large numbers globally distributed clinical isolates, suggest that this has diverged into at least three distinct genotypes designated European (E), Japanese (J), and mosaic (M). In the present study, we determined analyzed complete genomic sequences two M VZV strains compared them to E J retrieved from GenBank (including Oka vaccine preparation, V-Oka). Except for a few polymorphic tandem repeat...
Whole-genome sequencing of Cryptosporidium spp. is hampered by difficulties in obtaining sufficient, highly pure genomic DNA from clinical specimens. In this study, we developed procedures for the isolation and enrichment fecal specimens verification purity whole-genome sequencing. The were achieved a combination three oocyst purification steps amplification (WGA) purified oocysts. Quantitative PCR (qPCR) analysis WGA products was used as an initial quality assessment amplified DNA. assessed...
Vaccinia virus (VACV) has been implicated in infections of dairy cattle and humans, outbreaks have substantially impacted local economies public health Brazil. During a 2005 outbreak, VACV strain designated Serro 2 (S2V) was collected from 30-year old male milker. Our aim to phenotypically genetically characterize this Brazilian isolate. S2V produced small round plaques without associated comets when grown BSC40 cells. Furthermore, less virulent than the prototype VACV-Western Reserve (WR)...
Background Infections with Taenia solium are the most common cause of adult acquired seizures worldwide, and leading epilepsy in developing countries. A better understanding genetic diversity T. will improve parasite diagnostics transmission pathways endemic areas thereby facilitating design future control measures interventions. Microsatellite markers useful genome features, which enable strain typing identification complex pathogen genomes. Here we describe microsatellite characterization...
Bordetella holmesii, a human pathogen, can confound the diagnosis of respiratory illness caused by pertussis. We present draft genome sequences two B. holmesii isolates, one from blood, F627, and nasopharynx, H558. Interestingly, important virulence genes that are in pertussis not found holmesii.
Nonenzymatic genome replication has yet to be demonstrated experimentally. Recently, studies on the nonenzymatic primer extension mechanism mediated by nucleoside 5′-monophosphates (NMPs) activated with 2-aminoimidazole (2AI) have revealed that imidazolium-bridged dinucleotide intermediates (N * N) are responsible for bulk of chemical copying process. As a result, an efficacious synthetic pathway producing these substrates is desirable. Standard dehydrative redox reaction between NMPs and...
Abstract Nucleic acid conjugation methodologies involve linking the nucleic sequence to other (bio)molecules covalently. This typically allows for property enhancement whether it be therapeutic purposes, biosensing, etc. Here, we report a streamlined, aqueous compatible, on‐column methodology using acids containing site‐specific amino‐modifier. Both monophosphates and carboxylates were amenable strategy, allowing introduction of variety useful handles including azide, aryl, hydrophobic...
We developed a set of seven resequencing GeneChips, based on the complete genome sequences 24 strains smallpox virus (variola virus), for rapid characterization this human-pathogenic virus. Each GeneChip was designed to analyze divergent segment approximately 30,000 bases genome. This study includes hybridization results 14 strains. Of hybridized, only 7 had sequence information included in design GeneChips; similar remaining not tiled as reference these GeneChips. By use variola...
Background: Minority drug resistance mutations (DRMs) that are often missed by Sanger sequencing clinically significant, as they can cause virologic failure in individuals treated with antiretroviral therapy (ART) drugs. Objective: This study aimed to estimate the prevalence of minor DRMs among patients enrolled a Malawi HIV monitoring survey at baseline and one year after initiation ART. Methods: Forty-one plasma specimens collected from HIV-1 subtype C-positive seven clonal control samples...
Shiga toxin-producing Escherichia coli (STEC) is an important food-borne pathogen. Here, we report the draft whole-genome sequences of nine STEC strains isolated from clinical cases in United States. This first such information for serotypes O69, H11, O145:H25, O118:H16, O91:H21, O146:H21, O45:H2, O128:H2, and O121:H19.
Here, we report the complete genome sequences of three Legionella pneumophila subsp. pascullei strains (including both serogroup 1 and 5 strains) that were found in same health care facility 1982 2012.
Neisseria meningitidis serogroup B (MnB) was responsible for two independent meningococcal disease outbreaks at universities in the USA during 2013. The first University A New Jersey included nine confirmed cases reported between March 2013 and 2014. second outbreak occurred California, with four November public health response to these approval deployment of a vaccine that not yet licensed USA. This study investigated use whole-genome sequencing(WGS) examine genetic profile disease-causing...
Abstract Nonenzymatic genome replication is thought to be an important process for primitive lifeforms, but this has yet demonstrated experimentally. Recent studies on the nonenzymatic primer extension mechanism mediated by nucleoside 5′‐monophosphates (NMPs) activated with 2‐aminoimidazole have revealed that imidazolium‐bridged dinucleotide intermediates (N*N) account majority of chemical copying process. As a result, efficacious synthetic pathway producing substrates imidazoyl moiety...
Legionella pneumophila is the leading etiology of legionellosis infections in North America and Europe. Here we report draft genome sequence L. D-5864, a serogroup 6 strain, which was isolated from bronchial alveolar lavage specimen male patient Arizona 2009. Genes within lipopolysaccharide (LPS)-biosynthesis region could potentially be determinants specificity.
Here, we report the 187.8-kb genome sequence of Vaccinia virus Lister-Butantan, which was used in Brazil during WHO smallpox eradication campaign. Its showed an average similarity 98.18% with original Lister isolate, highlighting low divergence among related vaccine strains, even after several passages animals and cell culture.