C-Type Lectin Domain Family 3 Member B (CLEC3B), is down-regulated in serum and tumor tissues different cancers including hepatocellular carcinoma (HCC). However, the functions of CLEC3B HCC remains elucidated. The aim this study to analyze roles HCC. expression genes was evaluated by immunohistochemistry, western blot, real-time PCR, enzyme-linked immunosorbent assays, analysis on TCGA-LIHC database gene omnibus. Transmission electron microscopy immunofluorescence were applied detect...

Curcumin (CUR) is a natural polyphenolic compound with various pharmacological activities. Low water solubility and bioavailability limit its clinical application. In this work, to improve the of CUR, we prepared new co-crystal curcumin L-carnitine (CUR-L-CN) via liquid-assisted grinding. Both CUR L-CN have high safe dosages wide range applications in liver protection animal nutrition. The was fully characterized crystal structure disclosed. Dissolution experiments were conducted simulated...

Two packing polymorphs of drug–drug co-crystals between VD₂ and VD₃ exhibit dramatically different properties. Form A presents superior physicochemical properties compared with or themselves.

Progesterone (Prog), a natural steroid hormone, is widely used in birth control pills and menopausal hormone replacement therapies. Phloroglucinol (SPF) resveratrol (RSV) are also two kinds of active products that can be during pregnancy. Drug–drug cocrystals Prog with SPF RSV were designed successfully synthesized for purpose bringing improvements drug properties, including solubility, hydroscopic stability, color stability. The their hydrate structures systematically characterized by X-ray...

Abstract Rab3A is a small Ras-like GTPase critical for membrane traffic. Although the functions of have been reported in several cancers, roles hepatocellular carcinoma (HCC) never determined. To investigate potential HCC progression, we first determined levels tissues and observed upregulated mRNA protein most tumor tissues. However, vitro data showed that decreasing cell lines conferred no significant effects overexpressing PLC/PRF/5 cells even inhibited migration invasion. Meanwhile,...

Lutein (LT) is a natural carotenoid and widely used for its vision protection antioxidant activity. However, the long-chain polyene structure makes lutein sensitive to light oxygen poses many difficulties in production, processing, storage. In addition, special chemical of LT leads low solubility bioavailability. this study, we propose an efficient solution address these issues. A cocrystal with adipic acid (LT-APC) was obtained first time. The cocrystals were fully characterized. After...

Three new polymorphs (forms I, II, and III) of natural cholinesterase inhibitor Huperzine A were discovered through a comprehensive polymorph screening experiment. Their structures elucidated by single-crystal structure analysis characterized powder X-ray diffraction, Fourier transform-infrared spectroscopy, DXR Raman microscope. The physicochemical properties these forms investigated thermal (TGA DSC) dynamic vapor sorption (DVS) isotherms. dissolution rates compared with the marketed...

Huperzine A is a naturally occurring alkaloid used in clinical treatments for Alzheimer disease. The drug has also been widely dietary supplements improving memory loss and mental impairment. There are three polymorphs monohydrate form reported the literature. In present work, single crystal structures of new polymorphic four organic solvates were described. study was as an example to illustrate formation different, yet reoccurring packing patterns depending on inclusion solvent structures....

This investigation attempted to elucidate whether lncRNA PVT1-led miRNA axes participated in aggravating ozone-triggered asthma progression. One hundred and sixty-eight BALB/c mice were evenly divided into saline+air group, ovalbumin+air saline+ozone group ovalbumin+ozone group. Correlations evaluated between PVT1 expression airway smooth muscle function/inflammatory cytokine release among the models. Furthermore, pcDNA3.1-PVT1 si-PVT1 were, respectively, transfected CD4+T cells (ASMCs),...

Vitamin B9 gels were found to be pH-switchable by adding triethylamine or acetic acid. Such recyclable can used as novel co-crystallization media, resulting in four stoichiometric vitamin C co-crystals. Under highly supersaturated conditions, uniform microspheres of (VC)·(NA) with different particle sizes obtained through a spherical crystallization process.

Hepatocellular carcinoma (HCC) ranks as the sixth most prevalent cancer and third leading cause of tumor-related death, so it is urgently needed to discover efficient markers targets for therapy. β-1,3-N-acetylgalactosaminyltransferase II (B3GALNT2) belongs β-1,3-glycosyltransferases (b3GT) family has been reported regulate development both normal tumor tissues. However, studies on functions B3GALNT2 in are quite limited. Here we investigated potential role HCC progression. Western blot,...

Hydroquinone (HQ) is widely used in food stuffs and an occupational environmental pollutant. Although the hepatotoxicity of HQ has been demonstrated both vitro vivo, prevention HQ‐induced yet to be elucidated. In this study, we focused on intervention effect aqueous extracts Flos lonicerae Japonicae (FLJ) cytotoxicity. We that reduced cell viability a concentration‐dependent manner by administering 160 μ mol/L for 12 h as positive control The FLJ significantly increased decreased LDH...

What is the central question of this study? How does miR-22-3p exert a protective role in asthma? main finding and its importance? Upregulation hampered airway inflammation release inflammatory cytokines through blocking activation NLRP3-caspase-1-IL-1β signalling pathway asthma.Asthma, great public health burden, triggered by responses airways these are not addressed appropriately current therapies. This study aims to investigate regulatory mechanism microRNA-22-3p (miR-22-3p) on...

Coenzyme Q10 (CoQ10) exists in two forms, an oxidized form and a reduced form. Ubiquinol is the fully of CoQ10. Compared to form, ubiquinol has much higher biological absorption better therapeutic effect. However, important stability problem which hampers its storage formulation. It can be easily transformed into form-ubiquinone-even at low temperature. In this work, we designed, synthesized, characterized new cocrystal with vitamin B3 nicotinamide (UQ-NC). marketed exhibited excellent...

The available resources of Streptomyces represent a valuable repository bioactive natural products that warrant exploration. albulus is primarily utilized in the industrial synthesis ε-poly-L-lysine (ε-PL). In this study, NADP-dependent glyceraldehyde 3-phosphate dehydrogenase (GapN) from Streptococcus mutans was heterologously expressed S. CICC11022, leading to elevated intracellular NADPH levels and reduced NADH ATP concentrations. resulting perturbation metabolism comprehensively analyzed...

Abstract: Chlorophyll prediction facilitates the comprehension of red tide characteristics and enables early warning. In practice, it is formulated as a multivariate time series forecasting problem aimed at future chlorophyll concentrations by considering both exogenous factors chlorophyll. However, multi-step concentration poses formidable challenge due to intricate interaction between long temporal dependence input sequences. this work, we propose Multi-attention Recurrent Neural Network...

The metacestode (larval) stages of the cestode parasites Echinococcus vogeli and E. multilocularis were isolated from peritoneal cavity experimentally infected C57BL/6 mice cultured in vitro for a period up to 4 mo under conditions normally applied cultivation metacestodes. In contrast multilocularis, did not exhibit extensive exogenous budding proliferation but increased size with final diameter 10 mm. Most metacestodes contained protoscoleces, singly or groups, either associated brood...

A Gram-stain-positive, rod-shaped, endospore-forming, motile bacterium, designated D33T, was isolated from a forest soil sample. The strain grew optimally at 30–37 °C, pH 8.0 and with 1 % (w/v) NaCl. 16S rRNA gene sequence of the isolate showed similarities lower than 97 respect to species genus Paenibacillus. Strain D33T contained meso-diaminopimelic acid in cell-wall peptidoglycan, ribose amounts glucose galactose as whole-cell sugars. major cellular fatty anteiso-C15 : 0, menaquinone-7...

Benzo(a)pyrene (BaP) is a polycyclic aromatic hydrocarbon that specifically causes cancer and widely distributed in the environment. Poly (ADP-ribosylation), as key post-translational modification BaP-induced carcinogenesis, mainly catalyzed by poly (ADP-ribose) glycohydrolase (PARG) eukaryotic organisms. Previously, it found PARG silencing can counteract carcinogenesis vitro, but mechanism remained unclear. In this study, we further examined process vivo using heterozygous knockout mice...

Abstract Hepatocellular carcinoma (HCC) is a biologically aggressive cancer. Targeted therapy in need to tackle challenges the treatment perspective. A growing body of evidence suggests promising role pharmacological inhibition PIM (proviral integration site for Moloney murine leukaemia virus) kinase some human haematological and solid cancers. Yet date, potential application inhibitors HCC still largely unexplored. In present study we investigated pre‐clinical efficacy as therapeutic...

The freshwater bivalve Cristaria plicata, which is widely distributed in Eastern Asia, a key species the pearl culture industry. In this study, novel invertebrate-type lysozyme, designated as CpLYZ2, was cloned from hemocytes of C. plicata. This lysozyme shares high sequence identity and homologous to previously identified CpLYZ1 isolated plicata with HcLyso3 Hyriopsis cumingii. full-length cDNA CpLYZ2 913 bp long, includes an open reading frame (ORF) 486 bp, 3' untranslated region (UTR) 389...