A5001828674- Ubiquitin and proteasome pathways
- Hippo pathway signaling and YAP/TAZ
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Dental Implant Techniques and Outcomes
- Wnt/β-catenin signaling in development and cancer
- Peptidase Inhibition and Analysis
- Extracellular vesicles in disease
- Trypanosoma species research and implications
- Reproductive Biology and Fertility
- Genomics and Chromatin Dynamics
- Dental materials and restorations
- Parasites and Host Interactions
- Cellular Mechanics and Interactions
- Research on Leishmaniasis Studies
- Endodontics and Root Canal Treatments
- Advanced Nanomaterials in Catalysis
- Advanced biosensing and bioanalysis techniques
- DNA Repair Mechanisms
- Bone Tissue Engineering Materials
- Lipid metabolism and biosynthesis
- Cancer Research and Treatments
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- DNA and Nucleic Acid Chemistry
- Orthopaedic implants and arthroplasty
Heidelberg University
2000-2025
Heidelberg University
2025
DKFZ-ZMBH Alliance
2013-2019
Saarland University
2010
Institute of Human Genetics
2000
Meiotic maturation in vertebrate oocytes is an excellent model system for microtubule reorganization during M-phase spindle assembly. Here, we surveyed changes the pattern of microtubule-interacting proteins upon Xenopus laevis oocyte by quantitative proteomics. We identified synovial sarcoma X breakpoint protein (SSX2IP) as a novel protein. Using X. egg extracts, show that SSX2IP accumulated at poles Dynein-dependent manner and interacted with γ-tubulin ring complex (γ-TuRC) centriolar...
Extracellular vesicles (EV) are cell-derived, lipid bilayer-enclosed, non-replicable nanoparticles. EV currently gain attention in cardiovascular research due to their role regulating intercellular communication, potentially serving as valuable biomarkers for disease. However, the proteome and its potential a biomarker diagnostics remain poorly understood. This protocol presents standardized method isolation quantification of plasma-derived analysis protein cargo using plasma samples from...
Abstract The Escherichia coli Min system, composed of the proteins MinC, MinD, and MinE, is crucial for directing septum formation at mid-cell. MinD has also been implicated in chromosome segregation due to its non-sequence-specific DNA binding ability. Here, we demonstrate that MinCD complex, rather than alone, primary driver Min-mediated MinE regulates transient nature this interaction. We further reveal a critical function linker connecting MinC’s N- C-terminal domains both FtsZ...
The Hippo pathway transducers yes-associated protein (YAP) and WW-domain containing transcription regulator 1 (WWTR1/TAZ) are key regulators of liver tumorigenesis, promoting tumor formation progression. Although the first inhibitors in clinical trials, targeting relevant upstream YAP/TAZ activity could prove equally beneficial. To identify hepatocarcinoma (HCC) cells, we carried out a proximity labelling approach (BioID) coupled with mass spectrometry. We verified CRK-like proto-oncogene...
Abstract Background Adherens junctions (AJs) facilitate cell–cell contact and contribute to cellular communication as well signaling under physiological pathological conditions. Aberrant expression of AJ proteins is frequently observed in human cancers; however, how these factors tumorigenesis poorly understood. In addition, for some such α‐catenin contradicting data has been described. this study we aim decipher the constituent contributes liver cancer formation. Methods TCGA was used...
Trypanosomatids have a unique trypanothione-based thiol redox metabolism. The parasite-specific dithiol is synthesized from glutathione and spermidine, with glutathionylspermidine as intermediate catalyzed by trypanothione synthetase. In this study, we address the oxidative stress response of African trypanosomes special focus on putative protein S-thiolation.Challenging bloodstream Trypanosoma brucei diamide, H2O2 or hypochlorite results in distinct levels reversible overall S-thiolation....
In humans and plants, 40% of the proteome is cotranslationally acetylated at N-terminus by a single Nα-acetyltransferase (Nat) termed NatA. The core NatA complex comprised catalytic subunit 10 (NAA10) ribosome-anchoring NAA15. regulatory Huntingtin Yeast Partner K (HYPK) acetyltransferase NAA50 join this in humans. Even though both are conserved Arabidopsis (Arabidopsis thaliana), only AtHYPK known to interact with AtNatA. Here we uncover AtNAA50 interactome provide evidence for association...
The Hippo pathways effectors yes-associated protein (YAP) and WW domain containing transcription regulator 1 (WWTR1; syn: TAZ) play an important role in tumor development progression. Recent findings illustrate that both oncogenes facilitate their pro-tumorigenic properties liver cancer via common exclusive mechanisms. However, the underlying molecular network of YAP- TAZ-induced tumorigenesis is not fully understood. Here, we aim to dissect respective interactome cells.